The effects of Ascophyllum nodosum extract on the stimulation of germination indices in chickpea (Cicer arietinum) under drought stress

Ahmadpour, Raheleh; Salimi, Azam; ,; Armand, Nezam; Hosseinzadeh, Saeed Reza

doi:10.29252/nbr.6.2.206

Volume 6, Issue 2 (8-2019) nbr 2019, 6(2): 206-216 | Back to browse issues page

‎ 10.29252/nbr.6.2.206

‎ 20.1001.1.24236330.1398.6.2.7.4

Mendeley

Zotero

RefWorks

Ahmadpour R, Salimi A, Armand N, Hosseinzadeh S R. The effects of Ascophyllum nodosum extract on the stimulation of germination indices in chickpea (Cicer arietinum) under drought stress. nbr 2019; 6 (2) :206-216
URL: http://nbr.khu.ac.ir/article-1-2942-en.html

The effects of Ascophyllum nodosum extract on the stimulation of germination indices in chickpea (Cicer arietinum) under drought stress

Raheleh Ahmadpour

, Azam Salimi

, Nezam Armand

, Saeed Reza Hosseinzadeh ^*

Behbahan Khatam Alanbia University of Technology , hossinzadeh_tmu@yahoo.com

Abstract: (4907 Views)

In this study, the effects of seaweed extract (Ascophyllum nodosum) on seed germination stimulation and growth indices in chickpea were studied. A factorial experiment was performed on the basis of a completely randomized design with 3 replications. Treatments consisted of seaweed extract at four levels (0, 1.5, 2.5 and 3.5%) and drought stress at four levels (0, -0.3, -0.6 and -0.9 MP). Drought stress was induced by PEG 6000. Results showed that drought stress at -0.6 and -0.9 MP reduced the germination traits significantly compared with the controls. Seaweed extract at 2.5 and 3.5% resulted in a significant increase in the germination percentage (+18%, +24%), germination rate (+15%, +20%), seedling length (+13.3%, +15.7%), radicle area (+10.4%, +12.8%), radicle diameter (+14.2%, +25%), plumule length (+14%, +16.6%) and plumule dry weight (+10.3%, +12.6%). Under non-stress and -0.3 MP, seaweed extract levels at 2.5 and 3.5% could significantly increase the germination vigor, seed vigor index, consumed endosperm, radicle length and radicle dry weight. The application of seaweed extract at 3.5% improved the germination vigor, seed vigor index and radicle dry weight under -0.6 MP conditions. It also improved the consumed endosperm, length and radicle dry weight in samples affected by -0.9 MP conditions. According to the results, the use of seaweed extract (Ascophyllum nodosum) in the seed germination of beans is recommended for the reduction of the negative effects of drought stress.

Keywords: brown algae, consumed endosperm, legumes, organic fertilizers, PEG

Full-Text [PDF 868 kb] (1508 Downloads)

References

1. Agrawal, R.L. 1991. Seed technology. Oxford and IBH Publishing. 658 pp.

2. Ahmadpour, R., Armand, N., Hosseinzadeh, S.R and Chashiani, S. 2016. Selection drought tolerant cultivars of lentil (Lens culinaris Medik.) by measuring germination parameters. - Iranian J. Seed Sci. Res. 3: 75-88.

3. Amiri, H., Ismaili, A. and Hosseinzadeh, S.R. 2017. Influence of vermicompost fertilizer and water deficit stress on morpho-physiological features of chickpea (Cicer arietinum L. cv. karaj). - Compost Sci. Utili. 25: 152-165. [DOI:10.1080/1065657X.2016.1249313]

4. Armand, N., Amiri, H. and Ismaili, A. 2015. Effect of methanol on germination characteristics of bean (Phaseolus vulgaris L. cv. Sadry) under drought stress condition. - J. Puls. Res. 6: 42-53.

5. Bibi N., Hamed, A. and Ali, H. 2009. Water stress induced variations in protein profiles of germinating cotyledons from seedlings of chickpea genotypes. - Pakistan J. Bot. 41: 731-736.

6. Caffagni, D.E., Camargo, E., Casali, C.A., Lombardi, A.T. and Lima, M.I.S. 2015. Coupling microalgal cultures with hydroponics: Prospection for clean biotechnology processes. - J. Algal. Biomass. Util. 6: 88-94.

7. Craigie, J.S. 2011. Seaweed extract stimuliation in plant science and agriculture. - J. Appl. Phycol. 23: 371-393. [DOI:10.1007/s10811-010-9560-4]

8. David, C. 2010. The effect of gibberellins (GA3 and GA4) and ethanol on seed germination of Rosa eglanteria and Rosa glauca. - Plant Growth Regul. 41: 1-10.

9. Fabian, A., Jager, K. and Barnabas, B. 2008. Effects of drought and combined drought and heat stress on germination ability and seminal root growth of wheat (Triticum aestivum L.) seedlings. - Acta Bio. Szege. 52: 157-159.

10. Farooq, M., Basra S.M. and Ahmad, A.N. 2007. Imporoving the performance of transplanted rice by seed priming. - Plant Growth Regul. 51: 129-137. [DOI:10.1007/s10725-006-9155-x]

11. Gamze, O.K.U., Mehmet Demir, K.A.Y. and Mehmet, A.T.A. 2005. Effects of salt and drought stresses on germination and seedling growth of pea (Pisum sativum L.). - Turkish J. Agri. 29: 237-242.

12. Ghannad, R., Akbari, F. and Madadkar Haghjou, M. 2017. Effect of blue-green and green algae Spirulina, Chlorella, Dunaliella and minerals on the stimulation of metabolic and biochemical processes of germination in Dracocephalum kotschyi Boiss. Seeds. - Nova Biol. Reperta 3: 295-307. [DOI:10.21859/acadpub.nbr.3.4.295]

13. Gunes, A., Cicek, N., Inal, A., Alpaslan, M., Eraslan, F., Guneri, E. and Guzelordu, T. 2006. Genotypic response of chickpea (Cicer arietinum L.) cultivars to drought stress implemented at pre-and post anthesis stages and its relations with nutrient uptake and efficiency. - Plant Soil Environ. 52: 868-876. [DOI:10.17221/3454-PSE]

14. Halmer, P. 2000. Commercial seed treatment technology. In: Black, M. and Bewley, J. D (Eds.) Seed technology and its biological basis. - Sheffield Academic Press, Sheffield, 257-286.

15. Hosseinzadeh, S.R., Amiri, H. and Ismaili, A. 2016. Effect of vermicompost extract on germination characteristics of chickpea (Cicer arietinum L.) under drought stress. - J. Plant Res. 29: 589-598.

16. ISTA: International Seed Testing Association. 2009. International rules for seed testing. - Seed Sci. Tech. 49: 86-41.

17. Jannin, L., Arkoun, M., Etienne, P., Laıne, P., Goux, D. and Garnica, M. 2013. Brassica napus growth is promoted by Ascophyllum nodosum. Seaweed extract: microarray analysis and physiological characterization of N, C, and S metabolisms. - Plant Growth Regul. 32: 31-52. [DOI:10.1007/s00344-012-9273-9]

18. Kafi, M., Nezami, A., Hosaini, H. and Masomi A. 2005. Physiological effects of drought stress by polyethylene glycol on germination of lentil (Lens culinaris Medik.) genotypes. - Iranian J. Crops Res. 3: 69-80.

19. Kalefetoglu Macar, T., Turan, O. and Ekmekci, Y. 2009. Effect of water deficit induced by PEG and NaCl on Chickpea (Cicer arietinum L.) cultivars and lines at early seedling stage. - J. Sci. 22: 5-14.

20. Khalid, M.N., Iqbal, H.F., Tahir, A. and Ahmad, A.N. 2001. Germination potential of chickpeas (Cicer arietinum L.) under saline condition. - J. Bio Sci. 4: 395-396. [DOI:10.3923/pjbs.2001.395.396]

21. Kord Firouzjai, G., Habibi, H., Sodai Mashai, S. and Fotoukian, M.H. 2012. The effect of foliar application of fertilizers containing nutrients and growth stimulants on the germination factors of rice. - J. Sci. Tech. 2: 1-10.

22. Kumar, G. and Sahoo, D. 2011. Effect of seaweed liquid extract on growth and yield of Triticum aestivum var. Pusa Gold. - J. Appl. Phyco. 23: 251-255. [DOI:10.1007/s10811-011-9660-9]

23. Mensah, J.K., Obadoni, B.O., Eruotor, P.G. and Onome-Irieguna, F. 2006. Simulated flooding and drought effects on germination, growth and yield parameters of sesame (Sesamum indicum L.). - Africian J. Bio. 5: 1249-1253.

24. Michael, B.E. and Kaufman, M.R. 1976. The osmotic potential of polyethylenglycol-6000. - Plant Physiol. 51: 914-916. [DOI:10.1104/pp.51.5.914]

25. Rahbarian, R., Khavari-nejad, R., Ganjeali, A., Bagheri, A.R. and Najafi, F. 2012. Drought stress effect on germination and seedling for drought tolerance in chickpea genotypes (Cicer arietinum L.) under control condition. - Iranian J. Crops Res. 10: 522-531.

26. Ramarajan, S., Joseph, L.H. and Ganthi, A.S. 2012. Effect of seaweed liquid fertilizer on the germination and pigment concentration of soybean. - J. Crop Sci. Tech. 1: 1-5.

27. Zakaria, M.S., Ashraf, H.F. and Serag, E.Y. 2009. Direct and residual effects of nitrogen fertilization, foliar application of potassium and plant growth retardant on Egyptian cotton growth, seed yield, seed viability and seedling vigor. - Acta Eco. Sci. 29: 116-123. [DOI:10.1016/j.chnaes.2009.05.008]

28. Zhang, X.E. and Ervin, H. 2008. Impact of seaweed extract-based cytokinins and zeatin-riboside on creeping bentgrass heat tolerance. - Crop Sci. 48: 364-370. [DOI:10.2135/cropsci2007.05.0262]

29. Zhang, X.Z. and Ervin, E.H. 2004. Cytokinin-containing seaweed and humic acid extracts associated with creeping bentgrass leaf cytokinins and drought resistance. - Crop Sci. 44: 1737-1745. [DOI:10.2135/cropsci2004.1737]

30. Zodape, S.T. 2001. Seaweeds as a biofertilizer. - J. Sci. Indust. Res. 60: 378-382.