Volume 5, Issue 4 (3-2019)                   nbr 2019, 5(4): 438-448 | Back to browse issues page


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Afshar Mohammadian M, Kordi S. Comparative evaluation of enzymatic and non-enzymatic antioxidants of petal of three species of saffron (Crocus caspius, C. sativus, C. speciosus). nbr. 2019; 5 (4) :438-448
URL: http://nbr.khu.ac.ir/article-1-2888-en.html
University of Guilan , afshar@guilan.ac.ir
Abstract:   (800 Views)

Nowadays, the use of a wide range of medicinal herbs such as saffron and its aromatic compounds is increasingly being regarded as natural sources of antioxidant properties. In addition to saffron stigma, saffron petal is a rich herbal source of antioxidant compounds. The present study was conducted in a completely randomized design with three replications to study some of the enzymatic and non-enzymatic antioxidants of two species of wild saffron in Guilan Province compared with agronomic saffron (C. sativus). The results of this study showed that total phenol, flavonoids and anthocyanins in agronomic saffron were significantly higher than those in the other two species. The amount of flavonel in C. speciosus and the level of antioxidant activity (DPPH) in C. caspius were significantly higher than other species. The level of PPO activity in C. sativus and C. speciosus was significantly higher than that in C. caspius. However, there was no significant difference in the level of POD activity among the three studied species. Therefore, according to the results of this study, petals of different species of saffron are good sources of non-enzymatic and enzymatic antioxidants and can be used as an available natural antioxidant in the food and pharmaceutical industries.
 

Keywords: DPPH, flavonoid, phenol, POD, PPO
Full-Text [PDF 1027 kb]   (183 Downloads)    
Type of Study: Original Article | Subject: Plant Biology
Received: 2017/06/23 | Revised: 2019/04/6 | Accepted: 2018/09/26 | Published: 2019/03/18 | ePublished: 2019/03/18

References
1. Afraze, Z., Bolandi, M., Khorshidi, M. and Mohammadi, N.A. 2014. Evaluation of antioxidant activity of aqueous and alcoholic extracts (methanol, ethanol) saffron petals. – J. Saf. Agron. Technol. 2: 231-236.
2. Agha Hosseini, M., Kashani, L., Aleyaseen, A., Ghoreishi, A., Rahmanpour, H. and Zarrinara, A. 2008. Crocus sativus L. (saffron) in the treatment of premenstrual syndrome: a double‐blind, randomised and placebo‐controlled trial. – BJOG. 115: 515-519. [DOI:10.1111/j.1471-0528.2007.01652.x]
3. Akhondzadeh Basti, A., Moshiri, E., Noorbala, A.A., Jamshidi, A.H., Abbasi, SH. and Akhondzadeh, S. 2007. Comparison of petal of Crocus sativus L. and fluoxetine in the treatment of depressed outpatients: a pilot double-blind randomized trial. Progress Neuropsychopharmacology and Biological Psychi-atry. 31: 439-442. [DOI:10.1016/j.pnpbp.2006.11.010]
4. Anjum, N., Pal, A. and Tripathi, Y.C. 2015. Phytochemistry and pharmacology of saffron, the most precious natural source of colour, flavour and medicine. – SMU. 2: 335-346.
5. Bakhshi, D. and Arakawa, O. 2006. Induction of phenolic compounds biosynthesis with light irradiation in the flesh of red and yellow apples. – J. Appl. Horticul. 8: 101-104.
6. Beauchamp, C. and Fridovich, I. 1971. Superoxide dismutase: Improved assay and an assay applicable to acrylamide gels. – Anal. Biochem. 44: 276-282. [DOI:10.1016/0003-2697(71)90370-8]
7. Bergmeyer, H.U. 1974. Methods of Enzymatic Analysis 1, 2nd Edition, p: 495. – Academic Press, New York.
8. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. – Anal. Biochem. 72: 248-254. [DOI:10.1016/0003-2697(76)90527-3]
9. Delgrado, M.C., Aramburu, A. and Dìaz-Marta, G.L.A. 2006. The Chemical Composition of Saffron: Color, Taste and Aroma, Editorial Bomarzo, Albacete.
10. Fatehi, M., Rashidabady, T. and Fatehi- Hassanabad, Z. 2003. Effects of Crocus sativus petals' extract on rat blood pressure and on responses induced by electrical field stimulation in the rat isolated vas deferens and guinea-pig ileum. – J. Ethnopharmacol. 84: 199- 203. [DOI:10.1016/S0378-8741(02)00299-4]
11. Finkel, T. and Holbrook, N.J. 2000. Oxidants, oxidative stress and the biology of aging. – Natur. J. 408: 239-247. [DOI:10.1038/35041687]
12. Garido, J., Diez de Bethencourt, C. and Revilla, E. 1987. Flavonoid composition of hydrolysed tepal extracts of Crocus sativus L. – Anal. Bromatol. 39: 69-77.
13. Ghaeidamini, M., Shahrokhi, A., Khodakarimi, A. 2011. The impact of climate change on flowering behavior of saffron. National Conference on Climate Change and its Impact on Agriculture and Environment, Uremia.
14. Gill, S. S. and Tuteja N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. – Plant Physiol. Biochem. 48: 909-930. [DOI:10.1016/j.plaphy.2010.08.016]
15. Gregory, R.P.F. and Bendali, D.S. 1966. The Purification and some Properties of the Polyphenol Oxidase from Tea (Camellia sinensis L.). – Biochem. J. 101: 569. [DOI:10.1042/bj1010569]
16. Hadizadeh, F., Khalili, N., Hosseinzadeh, H. and Khair-Aldine, R. 2003. Kaempferol from Saffron Petals. – IJPR. 2: 251-252.
17. Hosseinzadeh, H. and Younesi, H.M. 2002. Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice. – BMC Pharmacol. 2: 7-15. [DOI:10.1186/1471-2210-2-7]
18. Kafi, M., Hemmati-Kakhki, A. and Karbasi, A. 2006. Historical background, economy, acreage, production, yield and uses. pp: 1-11. – In: Kafi, M., Koocheki, A., Rashed, M.H., Nassiri, M. (eds.). Saffron (Crocus sativus) Production and Processing. – Sci. Publishers, Enfield. [DOI:10.1201/9781482280463]
19. Kahl, R. and Kappus, H. 1993. Toxicity of synthetic antioxidants BHT and BHA in comparison with natural antioxidants vitamin E. – Z. Lebensm.Unters. Forsch. 196: 329-38. [DOI:10.1007/BF01197931]
20. Karimi, E., Oskoueian, E., Hendra, R. and Jaafar, H. Z. E. 2010. Evaluation of Crocus sativus L. stigma phenolic and flavonoid compounds and its antioxidant activity. – Molecules 15: 6244-6256. [DOI:10.3390/molecules15096244]
21. Kaveh, H. 2016. Effect of saffron petal extract on retention quality of fresh-cut watermelon cubes. – Saffron Agro. Tech. 4: 301-311
22. Krizek, D.T., Kramer, G.F., Upadyaya, A. and Mirecki, R.M. 1993. UV-B response of cucumber seedling grown under metal halide and high pressure sodium/deluxe lamps. – Physiol. Plantarum 88: 350-358. [DOI:10.1111/j.1399-3054.1993.tb05509.x]
23. Kubo, I. and I. Kinst-Hori 1999. Flavonols from Saffron Flower: Tyrosinase inhibitory activity and inhibition mechanism. – J. Agri. Food Chem. 47: 4121-4125. [DOI:10.1021/jf990201q]
24. Kulisic, T., Radonic, A. and Katalinic, V. 2004. Use of different methods for testing antioxidative of oregano essntial oil. – Food Chem. 85: 40-633. [DOI:10.1016/j.foodchem.2003.07.024]
25. Mahdavee Khazaei, K., Jafari, S.M., Ghorbani, M. and Hemmati Kakhki, A. 2014. Optimization of anthocyanin extraction in Saffron's petal with response surface methodology. – JRIFST. 3: 37-50.
26. Masukasu, H., Karin, O., and Kyoto, H. 2003. Enhancement of anthocyanin biosynthesis by sugar in radish (Raphanus sativus) hypocotyls. – Plant Sci. 164: 259-265. [DOI:10.1016/S0168-9452(02)00408-9]
27. Montoro, P., Tuberosob, C.I., Maldini, M., Cabrasb, P. and Pizza, C. 2008. Qualitative profile and quantitative determination of flavonoids from Crocus sativus L. petals by LC-MS/MS. – NPC. 3: 893-900. [DOI:10.1177/1934578X0800301215]
28. Moshiri, E., Basti, A.A., Noorbala, A.A., Jamshidi, A.H., Hesameddin Abbasi, S. and Akhondzadeh, S. 2006. Crocus sativus L. (petal) in the treatment of mildto- moderate depression: a double-blind, randomized and placebo-controlled trial. – Phytomedicine; 13: 607- 611. [DOI:10.1016/j.phymed.2006.08.006]
29. Nasirian, F., Sorush Zadeh, A., Ghanati, F., Oraki, H. 2014. The effect of root-zone temperature on antioxidant activities in saffron corm. – J. Saf. Agron. Technol. 2: 145-154.
30. Norbark, R., Brandt, K., Nielsen, J. K., girgaard, M. and Jacobsen, N. 2002. Flower pigment composition of crocus species and cultivars used for a chemotaxonomic investigation. – Biochem. Syst. Ecol. 30: 763-791. [DOI:10.1016/S0305-1978(02)00020-0]
31. Poli, G. and Parola, M. 1997. Oxidative damage and fibrogenesis. – Free Radic. Biol. Med. 22: 287-305. [DOI:10.1016/S0891-5849(96)00327-9]
32. Sarikurkcu, C., Tepe, B., Daferera, D., Polissiou, M. and Harmandar, M. 2008. Studies on the antioxidant activity of the essential oil and methanol extract of Marrubium globosum subsp. globosum (Lamiaceae) by three different chemical assays. – Bioresour. Technol. 99: 4239-4246. [DOI:10.1016/j.biortech.2007.08.058]
33. Sariri, R., Sabbaghzadeh, R. and Poumohamad, F. 2011. In-Vitro antioxidant and anti-tyrosinase activity of methanol extracts from Crocus Sativus flowers. – Pharmacol. 2: 1205-1215.
34. Sharififar, F., Moshafi, M.H. and Mansouri, S.H. 2007. In vitro evalution of antibacterial and antioxidant of the essential oil and methanol extract of endemic Zataria multiflora Boiss. – Food Cont. 18: 5-800. [DOI:10.1016/j.foodcont.2006.04.002]
35. Singh, G., Slinkard, K. and Singleton, V.L. 1977. Total phenol analyses: automation and comparison with manual methods. – Am. J. Enol. Viticul. 28: 49-559.
36. Singh Gill, S. & Tuteja, N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem. 48: 909-930. [DOI:10.1016/j.plaphy.2010.08.016]
37. Turkina, M. 2008. Functional proteomics of protein phosphorylation in algal photosynthetic membranes, pp: 1-58. – Linköping, Sweden.
38. Venskutonis, P.R., Miliauskas, G. and van Beek, T.A. 2004. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. – Food Chem. 85: 231-237. [DOI:10.1016/j.foodchem.2003.05.007]
39. Vuleta, A. and Tucic B. (2009) Thermal dependence of the antioxidant enzymes superoxide dismutase, catalase, and peroxidase in foliage of Iris pumila L. – Arch. Biol. Sci. 61: 441-446. [DOI:10.2298/ABS0903441V]
40. Yu, W., Zhao, Y. and Shu, B. 2004. The radical scavenging activities of radix pueraiae iso Flavonoids: Achemiluminescence study. – Food Chem. 86: 525-529. [DOI:10.1016/j.foodchem.2003.09.005]
41. Williams, C.A., Harborne, J.B. and Goldblatt, P. 1986. Correlations between phenolic patterns and tribal classification in the family iridaceae. – Phytochem. 25: 2135-2154. [DOI:10.1016/0031-9422(86)80079-6]

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