Volume 6, Issue 2 (8-2019)                   nbr 2019, 6(2): 140-147 | Back to browse issues page

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Baharara J, Ramezani T, Saghiri N, Salek F. Investigation apoptotic effects of silver nanoparticles coated with Achillea biebersteinii extract on A2780 ovarian cancer cells . nbr 2019; 6 (2) :140-147
URL: http://nbr.khu.ac.ir/article-1-2733-en.html
Investigation apoptotic effects of silver nanoparticles coated with Achillea biebersteinii extract on A2780 ovarian cancer cells
J-avad Baharara * , Tayebe Ramezani , Negar Saghiri , Farzane Salek
Mashhad Branch, Islamic Azad University, , baharara78@gmail.com
Abstract:   (5112 Views)

In recent years using of silver nanoparticles due to its unique properties was increased. Medical plant, the Achillea biebersteinii is rich in anti-cancers compounds. The aim of this study was to investigate the cytotoxicity effects of AgNPs synthesized using Achillea biebersteinii extract on human ovarian cancer A2780 cells. Cytotoxic effects of AgNPs with MTT test was performed at 48 hours in concentration of 2, 4, 6, 8, 16 and 32 µg/ml. To study the cell death induced by AgNPs DAPI, acridine orange (AO)/ Propidium iodide (PI) staining and Annexin v/ Propidium iodide assay and activation of caspase 3 and 9 were assessed. Results: The results showed that the synthesized AgNPs decreased cell viability dose dependently, calculated inhibitory concentration of 50 % (IC50) was 4 µg/ml.  The results from AO / PI, DAPI staining, Annexin V / PI showed that in treated cells the percentage of apoptotic cells compared with control increased. In addition, the treated cells showed increased activation of caspase 3/9. Hence silver nanoparticles induced cell death through the caspase dependent pathway. The results suggest that AgNPs synthesized using Achillea biebersteinii   extract exert their cytotoxic effect by inducing apoptosis.

Keywords: Nanoparticles, Green Synthesis, Medical plant
Full-Text [PDF 954 kb]   (1785 Downloads)    
Type of Study: Original Article | Subject: Cell and Molecular Biology
Received: 2017/01/18 | Revised: 2019/09/24 | Accepted: 2018/04/17 | Published: 2019/07/30 | ePublished: 2019/07/30
References
1. Ahamed, M., Karns, M., Goodson, M., Rowe, J., Hussain, SM., Schlager, J.J. and Hong Y. 2008. DNA damage response to different surface chemistry of silver nanoparticles in mammalian cells. - Toxicol. Appl. Pharmacol. 233: 404-410. [DOI:10.1016/j.taap.2008.09.015]
2. Ahmed, S., Ahmad, M., Swami, BL. and Ikram, S. 2016. A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: A green expertise. - J. Adv. Res. 7: 17-28. [DOI:10.1016/j.jare.2015.02.007]
3. Baharara, J,, Namvar, F., Ramezani, T., Hosseini, N. and Mohamad, R. 2014. Green synthesis of silver nanoparticles using Achillea biebersteinii flower extract and its anti-angiogenic properties in the rat aortic ring model. - Molecules 19: 4624-34. [DOI:10.3390/molecules19044624]
4. Baharara, J., Namvar, F., Ramezani, T., Mousavi, M. and Mohamad, R. 2015. Silver nanoparticles biosynthesized using Achillea biebersteinii flower extract: apoptosis induction in MCF-7 cells via caspase activation and regulation of bax and bcl-2 gene expression. - Molecules 20: 2693-2706. [DOI:10.3390/molecules20022693]
5. Bexiga, M.G., Varela, J.A., Wang, F., Fenaroli, F., Salvati, A., Lynch, I., Simpson, J.C. and Dawson, K.A. 2011. Cationic nanoparticles induce caspase 3-, 7- and 9-mediated cytotoxicity in a human astrocytoma cell line. - Nanotoxicology 5: 557-567. [DOI:10.3109/17435390.2010.539713]
6. Brzóska, K., Męczyńska-Wielgosz, S., Stępkowski, T.M. and Kruszewski, M. 2015. Adaptation of HepG2 cells to silver nanoparticles-induced stress is based on the pro-proliferative and anti-apoptotic changes in gene expression. - Mutagenesis 30: 431-439. [DOI:10.1093/mutage/gev001]
7. Dos- Santos, C.A., Seckler, M.M., Ingle, A.P., Gupta, I., Galdiero, S., Galdiero, M., Gade, A. and Rai, M. 2014. Silver nanoparticles: Therapeutical uses, toxicity, and safety issues. - J. Pharm. Sci. 103: 1931-1944. [DOI:10.1002/jps.24001]
8. Faedmaleki, F., Shirazi, F., Salarian, A., Ahmadi- Ashtiani, H. and Rastegar, H. 2014. Toxicity effect of silver nanoparticles on mice liver primary cell culture and hepg2 cell line. - Iran. J. Pharm. Res. 13: 235-242.
9. Hughes, M.P., Alimova, A., Cnnbpiydp, N. and Awm, Z. 2000. AC electrokinetics: applications for nanotechnology. - Nanotechnology 11: 124-132. [DOI:10.1088/0957-4484/11/2/314]
10. Iravani, A., Korbekandi, H., Mirmohammadi, S.V. and Zolfaghari, S .2014. Synthesis of silver nanoparticles: chemical, physical and biological methods. - Res. Pharm. Sci. 9: 385-406.
11. Jeong, J., Gurunathan, S. and Kang, M. 2016. Hypoxia-mediated autophagic flux inhibits silver nanoparticle- triggered apoptosis in human lung cancer cells. - Sci. Rep. 6: 21668. [DOI:10.1038/srep21688]
12. Kalishwaralal, K., Banumathia, E., Kumar, S.R., Pandiana, V., Deepaka, J., Muniyandia, S.H., Eomb, S. and Gurunathan, H. 2009. Silver nanoparticles inhibit VEGF induced cell proliferation and migration in bovine retinal endothelial cells. - Colloids Surf B: Biointerfaces 73: 51-57. [DOI:10.1016/j.colsurfb.2009.04.025]
13. Marambio-Jones, C. and Hoek, V. 2010. A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment. - J. Nanopart Res. 12: 1531-1551. [DOI:10.1007/s11051-010-9900-y]
14. Mazandarani, M., Osia, N. and Ghafourian, M. 2015. Antioxidant activity and ethno pharmacological survey of Achillea biebersteinii afan in the treatment of dysmenorrhoea in traditional medicine of Golestan province, Iran. - Int. J. Womens Health Reprod Sci. 3: 107-110. [DOI:10.15296/ijwhr.2015.21]
15. McShan, D., Ray, P.C. and Yu, H. 2014. Molecular toxicity mechanism of nanosilver. - J. Food Drug Anal. 22: 116-127. [DOI:10.1016/j.jfda.2014.01.010]
16. Prabhu, S. and Poulose, E.K. 2012. Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. - Int. Nano Lett. 2: 32. [DOI:10.1186/2228-5326-2-32]
17. Rao, P.V., Nallappan, D., Madhavi, K., Rahman, S., Jun- Wei, L. and Gan, S.H. 2016. Phytochemicals and Biogenic Metallic Nanoparticles as Anticancer Agents. - Oxidative Med. Cell. Longev. 5: 1-15 [DOI:10.1155/2016/3685671]
18. Satapathy, S., Mohapatra, P., Preet, R., Das, D., Sarkar, B., Choudhuri, T., Wyatt, M. and Kundu, C .2013. Silver-based nanoparticles induce apoptosis in human colon cancer cells mediated through p53. - Nanomedicine 8: 1307-1322. [DOI:10.2217/nnm.12.176]
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