Volume 4, Issue 4 (3-2018)                   nbr 2018, 4(4): 373-379 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Lovett D, Abroumand A. Digestive enzyme activity of western white shrimp (Litopenaeus vannamei) in different life stages . nbr 2018; 4 (4) :373-379
URL: http://nbr.khu.ac.ir/article-1-3031-en.html
Digestive enzyme activity of western white shrimp (Litopenaeus vannamei) in different life stages
Donald Lovett , Ali Abroumand
Abstract:   (4746 Views)
Gastrointestinal ontogenetic studies constitute one of the basic and important investigations related to the nutrition of aquatic animals. In this investigation, specimens of the western white shrimp (Litopenaeus vannamei) at different developmental stages (from nauplius 1 to postlarvae 120) were assayed for the activities of digestive enzymes. According to the results, at all developmental stages, trypsin, amylase, and lipase enzymes were found to be active. In addition, the peak activities of all enzymes were revealed to occur during the late zoea larval stages (Z3). On the other hand, minimum activities were observed to occur at metamorphosis. During the postlarval developmental stages, amylase and lipase activities increased steadily, whereas the trypsin activity was more or less constant up to the eighteenth week. In conclusion, ontogenetic change in digestive enzyme activity may reflect either a developmentally cued change in enzyme synthesis or a secondary effect of change in the function and relative size of the midgut during its differentiation.
 
 
 
 
Keywords: amylase, enzyme extracts, lipase, trypsin
Full-Text [PDF 615 kb]   (1619 Downloads)    
Type of Study: Original Article | Subject: Animal Biology
Received: 2016/08/20 | Revised: 2018/05/9 | Accepted: 2017/12/16 | Published: 2018/03/19 | ePublished: 2018/03/19
References
1. Bernfeld, P. 1955. Amylase. In: Colowick, S.P., Kaplan, N.O. (Eds.), Methods in enzymology. – Academic Press, New York, pp 149-158.
2. Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. – Anal. Biochem. 72: 248-254.
3. Buddington, R.K., and Doroshov, S.I. 1986. Deve-lopment of digestive secretions in white sturgeon juveniles (Acipenser transmontanus). – Camp. Biochem. Physiol. 83: 233-238.
4. Del Toro, M., García-Carreño, F., and Córdova-Murueta, J.H. 2011. Comparison of digestive proteinases in three penaeids. – Aquacult. 317: 1-4.
5. Hernández, J.C.S., Murueta, J.H.C. 2009. Activity of trypsin from Litopenaeus vannamei. – Aquacult. 290: 3-4.
6. Kuz'mina, V.V., Skvortsova, E.G., Zolotareva, G.V., and Sheptitskiy, V.A. 2011. Influence of pH upon the activity of glycosidases and proteinases of intestinal mucosa, chyme and microbiota in fish. – Fish Physiol. Biochem. 37: 345-353.
7. Laubier-Bonichon, A., Van Wormhoudt, A., and Sellos. D. 1977. Croissance larvaire controlee de Penaeus japonicus Bate: enzymes digestives et changements de regimes alimentaires. – Act. Coll. CNEXO 4: 131-145.
8. Lovett, D.L., and Felder, D.L. 1990. Ontogenetic change in digestive enzyme activity of larval and postlarval white shrimp Penaeus setiferus (Crustacea, Decapoda, Penaeidae). – Biol. Bull. 178: 144-159.
9. Muhlia-Almazán, A., Sánchez-Paz, A., and García-Carreño, F.L. 2008. Invertebrate trypsins: a review. – J. of Comp. Physiol. 178: 655-672.
10. Naegel, L.C., and Rodríguez-Astudillo, S., 2004. Comparison of growth and survival of white shrimp postlarvae (Litopenaeus vannamei) fed dried Artemia biomass versus four commercial feeds and three crustacean meals. – Aquacult. Intern.12: 573-581.
11. Rungruangsak-Torrissen, K., Rustad, A., Sunde, J., Eiane, S.A., Jensen, H.B., Opstvedt, J., Nygard, E., Samuelsen, T.A., Mundheim, H., Luzzana, U. and Venturini, G. 2002. In vitro digestibility based on fish crude enzyme extract for prediction of feed quality in growth trials. – J. Sci. Food Agric. 82: 644-654.
12. Vacquier, V.D., Kern, L.J., and Epel. D. 1971. The appearance of α-amylase activity during gut differentiation in sand dollar plutei. – Dev. Biol. 26: 393-399.
13. Van Wormhoudt, A. 1973. Variations des proteases, des amylases, et des proteines soluble au curs du developpement larvaire chez Palaemon serratus. – Mar. Biol. 19: 245-248.
14. Worthington, C.C. 1991. Worthington Enzyme Manual. – 3rd Edition. Worthington Biochemical Corp. Freehold, New Jersey.
15. Wyban, J., Walash, W.A., and Godin, D.M. 1999. Temperature effect on growth, feeding rate and feed conversion of the pacific white shrimp. – Aquacult. 138: 267-279.
17. Ziaeinejad, S., Rezaei, M.H., Takami, G.A., Lovett, D.L., Mirvaghefi, A.R., and Shakouri, M. 2006. The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity, survival and growth in the Indian white shrimp Fenneropenaeus indicus. – Aquacult. 252: 516-524.
Add your comments about this article : Your username or Email:
CAPTCHA
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Creative Commons Licence
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.



© 2024 CC BY-NC 4.0 | Nova Biologica Reperta

Designed & Developed by : Yektaweb