Nova Biologica Reperta
یافتههای نوین در علوم زیستی
NBR
Basic Sciences
http://nbr.khu.ac.ir
1
admin
2423-6330
2476-7115
10.22034/nbr
en
jalali
1400
4
1
gregorian
2021
7
1
8
2
online
1
fulltext
fa
بررسی خواص آنتیاکسیدانی و ضد سرطانی کمپلکسهای کورکومين/ بتا- و گاما-سيكلودكسترين اصلاح شده با نانوذرات کیتوزان بر روی رده سلولی سرطان ریه A549
Evaluation of antioxidant and anti-cancer properties of curcumin / beta- and gamma-cyclodextrin complexes modified with chitosan nanoparticles on lung cancer cell A549
بیوتکنولوژی
Biotechnology
مقاله پژوهشی
Original Article
<span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">هدف</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">اين</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">مطالعه</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">بررسي</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">بر­هم­كنش اصلاح</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">مولكول کمپلکس</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">کورکومين</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">(CUR) </span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> در حامل­هاي بتا- و</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">گاما</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"> –</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">سيكلودكسترين (</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">β-CD</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> و </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">γ-CD</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">) با نانو­ذرات کیتوزان</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">(CS) </span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> جهت</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">دارورساني</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">هدفمند</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">و</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">مقايسه</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">كاركرد</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">بين</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">آنها</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">بود</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">.</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> سیستم تحویل هدفمند دارویی شامل عامل درمانی نانوذرات </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">CS</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> بخش هدفگیری همان دارو و سیستم حامل </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">CD</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> است.</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">محاسبات روابط تشکیل کمپلکس­های اصلاح شده و کاربرد آنها با</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">استفاده</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">آنالیز داده­های اسپکتروسکوپی </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">UV-vis</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> انجام</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">شد. در</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">اين</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">مطالعه</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">نمودارهای طیف­های اسپکتروسکوپی­ها برای اثبات بهينهسازي</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">ساختار مولکولی در کمپلکسهای اصلاح شده رسم</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">شدند. تحليل</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">دادههای بررسی کاربرد آنها با روش معادلات مربوطه انجام</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">گرفت</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">.</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> پلیساکارید کاتیونی </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">CS</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> با حضور عامل­های آمینو و الکل در طول زنجیره­های پلی­ساکارید آن را قادر می­سازد تا پیوند ثابت کووالانسی را با کمپلکس­ها ایجاد کند و زمینه حلالیت سیکلودکسترین را افزایش دهد. نانوذرات </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">CS</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> به واسطه پیوند­های هیدروژنی و بر­هم­کنش­های واندروالس هيدروژن</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">گروه</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">هيدروكسيل</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">سيكلودكسترين</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">با هیدروکسیل</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">گروه</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">فنولیک</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">مولكول</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">دارويي </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">CUR</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> تقویت پيوند هيدروژني</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">برقرار</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">مي</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">­</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">كند. اصلاح کمپلکس </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">γ-CD</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> با </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">CS</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> قويترين</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">بر­هم­كنش</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">را</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">با</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"> CUR </span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> نشان</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">مي­دهد.</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">هر دوی</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">كمپلكس­هاي </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">CUR</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> در سیستم میزبان </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">CD-CS</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> جهت</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">انتقال</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">بار</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">دارو</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">به</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">حامل و عامل درمانی</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">است</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">.</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> نانوذرات </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">CS</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> خاصیت سیستم­های تحویل هدفمند داروهای ضد سرطان را دارند. زیرا میدان خارجی </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">CS</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> را میتوان برای هدایت دارو به سمت سلولهای هدف خاص مورد استفاده قرار داد. سیستم میزبان </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">γ-CD-CS</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> به</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">دليل</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">حلاليت</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">زياد</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">و</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">بر­هم­كنش</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">قوي</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">آن</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">بهترين میزبان</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">به­عنوان</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">حامل و عامل درمانی برای</span></span> <span style="font-family:B Nazanin;"><span style="font-size:10.0pt;">داروي </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">CUR</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:10.0pt;"> است</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">.</span></span></span><strong><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:13.0pt;"></span></span></span></strong><br />
<br />
<br />
<span dir="LTR"></span><span dir="LTR"></span>
The aim of this study was to investigate the interaction modification of curcumin complex molecule (CUR) in beta- and gamma-cyclodextrin (β-CD and γ-CD) carriers with chitosan (CS) nanoparticles for targeted drug delivery and to compare their performance. The targeted drug delivery system includes the therapeutic agent of the CS nanoparticles targeting section of the same drug and the CD carrier system. Calculations of the relationships of the formation of modified complexes and their application were performed using UV-vis spectroscopic data analysis. In this study, spectroscopic spectrum diagrams were drawn to prove the optimization of molecular structure in the modified complexes. Data analysis was performed using their respective equations. The cationic polysaccharide CS, with the presence of amino agents and alcohols along the polysaccharide chains, enables it to form a covalent bond with the complexes and increase the solubility of cyclodextrin. CS nanoparticles strengthen the hydrogen bond by hydrogen bonding and van der Waals hydrogen interactions of the hydroxyl cyclodextrin group with the hydroxyl phenolic group of the drug molecule CUR. Modification of the γ-CD complex with CS shows the strongest interaction with CUR. Both CUR complexes are in the CD-CS host system to transfer the charge from the drug to the carrier and the therapeutic agent. CS nanoparticles have the property of targeted delivery systems for anticancer drugs because the CS external field can be used to direct the drug to specific target cells. The γ-CD-CS host system is the best host as a carrier and therapeutic agent for CUR due to its high solubility and strong interaction.<span dir="RTL"></span><br />
<br />
حاملهای دارو, سیکلودکسترین, ضدسرطانی, کورکومین و نانوذرات کیتوزان
anticancer, chitosan nanoparticles, curcumin, cyclodextrin, drug carriers
84
94
http://nbr.khu.ac.ir/browse.php?a_code=A-10-1287-1&slc_lang=fa&sid=1
Nina
Alizadeh
نینا
علیزاده
n-alizadeh@guilan.ac.ir
10031947532846009050
10031947532846009050
Yes
Department of Chemistry, Faculty of Science, University of Guilan, P.B. 41335–1914, Rasht, Iran; Department of Chemistry, Faculty of Science, University Campus 2, University of Guilan, Rasht, Iran
گروه شیمی تجزیه، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران؛ گروه شیمی تجزیه، دانشکده علوم پایه، پردیس دانشگاهی دانشگاه گیلان، رشت، ایران
Shokufeh
Malakzadeh
شکوفه
ملک زاده
naerehb@yahoo.com
10031947532846009051
10031947532846009051
No
Department of Chemistry, Faculty of Science, University Campus 2, University of Guilan, Rasht, Iran
گروه شیمی تجزیه، دانشکده علوم پایه، پردیس دانشگاهی دانشگاه گیلان، رشت، ایران