en مقایسه سه روش رنگ آمیزی مختلف برای توصیف مورفومتریک اسپرم آلپاکا (Vicugna pacos) با استفاده از سیستم ISAS® CASA-Morph علوم جانوری Animal Biology مقاله پژوهشی Original Article <span style="font-family:b zar;"><span style="font-size:10.0pt;">مورفومتری</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">اسپرم</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">بخشی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">آنالیز</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مایع</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">منی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مبتنی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">بر</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">فناوری</span></span> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">CASA</span></span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;"> است</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">و</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">نقش</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مهمی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">پیش&shy; بینی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">باروری</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مردان</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">نشان</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">داده</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">است. این</span></span> <span style="color:black;"><span style="font-family:b zar;"><span style="font-size:10.0pt;">آ</span></span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">نالیز</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">برپا</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">یه</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">استفاده</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">تكنيك</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">هاي</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مختلف</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">رنگ &shy;آمیزی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">است</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">،</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">اگرچه</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">نشان</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">داده</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">شده</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">است</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">که</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">رنگ </span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">&shy;</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">آمیزی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">می</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">تواند</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">تغییرات</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">چشمگیری</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مورفومتری</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">سلول</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">گونه&shy; های</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مختلف</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">ایجاد</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">کند. هدف</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">این</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مطالعه،</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">بررسی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">تفاوت</span></span><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">‌</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">های</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">موجود</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مورفومتري</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">اسپرم</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">در</span></span><span style="color:black;"><span style="font-family:b zar;"><span style="font-size:10.0pt;">آلپاکا</span></span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">بود</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">كه</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">با</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">استفاده</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">تكنيك </span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">&shy;</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">هاي</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مختلف</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">رنگ &shy;آمیزی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">ا</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">نجام</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">شد. </span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">یک</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">انزال</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">از</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">هریک</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">پنج</span></span> <span style="color:black;"><span style="font-family:b zar;"><span style="font-size:10.0pt;">آلپاکای</span></span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">بالغ</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مورد</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">استفاده</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">قرار</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">گرفت. نمونه</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">&shy;</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;"> ها</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">با</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">استفاده</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">روش</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">جراحی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مجرای</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">دفران</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">جمع</span></span><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">‌</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">آوری</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">شدند</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">. سه</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">روش</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">رنگ</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">&shy;</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;"> آمیزی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">(یعنی</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Hemacolor </span></span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">،</span></span> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Harri&rsquo;s Henatoxylin</span></span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;"> و</span></span> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Diff-Quik</span></span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">) مورد</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">بررسی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">قرار</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">گرفت. تجزیه</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">و</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">تحلیل</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مورفومتریک</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">با</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">استفاده</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">سیستم</span></span> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">ISAS&reg; v1 CASA-Morph</span></span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">،</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">با</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">بزرگنمایی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">100</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">x</span></span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">و</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">میکروسکو</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">پ</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">زمینه</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">روشن</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">و</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">یک</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">دوربین</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">فیلمبرداری</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">دیجیتالی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">با</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">وضوح</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">نهایی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">08/0 میکرومتر</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;"> بر</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;"> پیکسل</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">انجام</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">شد. تقریباً</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">تعداد</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">200 سلول</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">به</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">صورت</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">تصادفی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">هر</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">نمونه</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">انتخاب</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">شد</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">وانتخاب</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">تکنیک</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">رنگ &shy;آمیزی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">با</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">توجه</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">به</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">اهمیت</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">پارامتر</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">های</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">اندازه</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">سر</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">اسپرم</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">و</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">بخش</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">میانی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">انتخاب</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">شد</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">.</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;"> بین</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">تکنیک &shy;های</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">به</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">کار</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">رفته</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">تقریباً</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">کلیه</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">پارامترها</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">سطوح</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">متفاوت</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">تفاوت&shy; ها</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">یی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">را</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">خود</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">نشان</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">داد</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">ند</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">. به</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">طور</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">کلی،</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">سلول&shy; هایی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">که</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">سر</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">بزرگ</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">داشتند</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">با</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">تکنیک</span></span> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Harri&rsquo;s Henatoxylin</span></span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;"> و</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">آن</span></span><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">‌</span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;">هایی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">که</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">دارای</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">سر</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">کوچک</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">بودند</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">با</span></span> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Diff-Quik</span></span></span><span style="font-family:b zar;"><span style="font-size:10.0pt;"> رنگ</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">آمیزی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">شدند. پارامترهای</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">تفکیک</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">کننده</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">برای</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">تمایز</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">بهتر</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">بین</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">حیوانات،</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">عرض</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">سر،</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">سطح</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">و</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">درصد</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">آکروزوم</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">بود. در</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">نتیجه،</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مانند</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">سایر</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">گونه ها،</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">نتایج</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مورفومتری</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">اسپرم</span></span> <span style="color:black;"><span style="font-family:b zar;"><span style="font-size:10.0pt;">آلپاکا</span></span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">نیز</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">به</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">تکنیک</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">رنگ &shy;آمیزی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">به</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">کاررفته،</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">حساس</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">بود. این</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">دلالت</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">بر</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">لزوم</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">ذکر</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">واضح</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">و</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">دقیق</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">تکنیک</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">رنگ &shy;آمیزی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">استفاده</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">شده</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">هر</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مورد</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">برای</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">انجام</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مقایسه</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">بین</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مطالعات</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مختلف</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">روی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">گونه&shy; های</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مشابه</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">دارد. هیچ</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">یک</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">تکنیک &shy;های</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">به</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">کار</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">گرفته</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">شده</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">برتر</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">سایرین</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">نبود. علاوه</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">بر</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">این،</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">روشی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">که</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">برای</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">به</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">دست</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">آوردن</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">نمونه&shy; ها</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">استفاده</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">شد،</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">سودمندی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">و</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">سادگی</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">آن</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">را</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">برای</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">نمونه</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">برداری&shy; های</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">مکرر</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">نشان</span></span> <span style="font-family:b zar;"><span style="font-size:10.0pt;">داد.</span></span> <p class="western" style="margin-bottom: 0.35cm; line-height: 100%">Sperm morphometry is a part of the semen analysis based on CASA technology and has shown a big role in the prediction of male fertility. This analysis implies the use of stain techniques, although it has been shown that staining can make dramatic changes in the cell morphometry in different species. The aim of the present work was to evaluate the differences in sperm morphometry in Alpaca, introduced by the use of different stain techniques. Single ejaculates from five adult alpacas were used. Samples were recovered by deferent duct deviation surgery technique. Three stain techniques (i.e. Hemacolor, Harri&rsquo;s Henatoxylin and Diff-Quik) were evaluated. Morphometric analysis was done using ISAS&reg; v1 CASA-Morph system, at 100x bright field objective magnification and a digital video camera with a final resolution of 0.08 &micro;m/pixel. Almost 200 randomly selected cells were automatically analysed per sample and stain technique, considering several sperm-head and midpiece parameters regarding size and shape. Almost all of the parameters showed different levels of difference among the employed techniques. In general, the largest cell heads were the ones stained with Harri&rsquo;s Hematoxylin and the smallest ones were the ones stained with Diff-Quik. The discriminant parameters used to differentiate between animals better were the head width, area and acrosome percentage. In conclusion, like in other species, alpaca sperm morphometry results were found to be sensitive to the applied stain technique. This implies the necessity of referring clearly the stain technique used in each case to perform comparisons between different works on the same species. None of the employed techniques was superior to the others. In addition, the method used to obtain the samples showed its usefulness and simplicity for repeated samplings.<br /> &nbsp;<br /> &nbsp;<br /> &nbsp;</p> آنالیز منی, دیف کوئیک, ریخت شناسی اسپرم, هما کالر, هماتوکسیلین هری Diff-Quik, Hemacolor, Harri’s Hematoxylin, semen analysis, sperm morphometry 284 291 http://nbr.khu.ac.ir/browse.php?a_code=A-10-946-1&slc_lang=en&sid=1 Hernán Cucho هرنان کوچو 10031947532846007123 10031947532846007123 Yes Universidad Nacional de San Antonio Abad Del Cusco دانشگاه ملی سن آنتونیو آباد دل کوسکو Yolvi López یولوی لوپز 10031947532846007124 10031947532846007124 No Universidad Nacional de San Antonio Abad Del Cusco دانشگاه ملی سن آنتونیو آباد دل کوسکو Carina Caldeira کارینا کالدیرا 10031947532846007125 10031947532846007125 No 2PROISER R+D شرکت پرویسر Anthony Valverde آنتونی والورده 10031947532846007126 10031947532846007126 No University of Valencia دانشگاه والنسیا César Ordóñez سزار اوردونز 10031947532846007127 10031947532846007127 No Universidad Nacional de San Antonio Abad Del Cusco دانشگاه ملی سن آنتونیو آباد دل کوسکو Carles Soler کارلس سولر carles.soler@uv.es 10031947532846007128 10031947532846007128 No University of Valencia دانشگاه والنسیا