fa اثر ضد میکروبی عصاره آبي سه گونه مريم­ گلی بر باکتری­های استافیلوکوکوس ­اورئوس، اشرشیاکولی و سودوموناس آئروجینوزا میکروبیولوژی Microbiology مقاله پژوهشی Original Article <p><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">در این مطالعه اثر عصاره آبی سه گونه </span></span><em><span dir="LTR"><span style="font-family:b-zar,serif;"><span style="font-size:10.0pt;">Salvia perspolitana </span></span></span></em><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">، </span></span><em><span dir="LTR"><span style="font-family:b-zar,serif;"><span style="font-size:10.0pt;">S. palaestina</span></span></span></em> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">و </span></span><em><span dir="LTR"><span style="font-family:b-zar,serif;"><span style="font-size:10.0pt;">S. bracteata</span></span></span></em> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">بر باکتری&shy; های بیماری&shy; زای </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Staphylococcus aureus</span></span></span></em><em><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">، </span></span></em>&nbsp;<em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Escherichia coli</span></span></span></em><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> و</span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Pseudomonas aeroginosa</span></span></span></em><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> تحت مطالعه قرار گرفت. جهت بررسی خاصیت ضدمیکروبی عصاره اين گیاهان از روش &shy;های انتشار چاهک-پلیت و حداقل غلظت بازدارندگی رشد (</span></span><span dir="LTR"><span style="font-family:b-zar,serif;"><span style="font-size:10.0pt;">MIC</span></span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">) استفاده شد. نتایج نشان داد که فقط عصاره </span></span><em><span dir="LTR"><span style="font-family:b-zar,serif;"><span style="font-size:10.0pt;">S. bracteata</span></span></span></em> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">در پلیت</span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Staphylococcus aureus </span></span></span></em><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">&nbsp;هاله &shy;عدم&shy; رشد به &shy;قطر 9 میلی&shy;متر تشکیل داد. عصاره هر سه گياه در </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Escherichia coli</span></span></span></em><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> و </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Pseudomonas aeroginosa</span></span></span></em><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> هاله &shy;عدم&shy; رشد تشكيل دادند. تاثير عصاره</span></span> <em><span dir="LTR"><span style="font-family:b-zar,serif;"><span style="font-size:10.0pt;">S. bracteata </span></span></span></em><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">با تشكيل هاله عدم رشد به &shy;قطر 10 میلی &shy;متر از ساير گونه&shy; ها مؤثر&shy;تر بود. نتایج </span></span><span dir="LTR"><span style="font-family:b-zar,serif;"><span style="font-size:10.0pt;">MIC</span></span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> نشان داد كه عصاره </span></span><em><span dir="LTR"><span style="font-family:b-zar,serif;"><span style="font-size:10.0pt;">S. perspolitana</span></span></span></em><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> اثر ضعيفي بر باکتری </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Staphylococcus aureus</span></span></span></em><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> داشت و اثر بازدارندگي در غلظت</span></span> <span dir="LTR"><span style="font-family:courier new;"><span style="font-size:10.0pt;">&micro;</span></span></span><span dir="LTR"><span style="font-family:b-zar,serif;"><span style="font-size:10.0pt;">g/ml </span></span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">&nbsp;1024</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">مشاهده شد. عصاره اين گونه، همچنين در غلظت </span></span><span dir="LTR"><span style="font-family:b-zar,serif;"><span style="font-size:10.0pt;">&micro;g/ml</span></span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> 256 بر باکتری&shy;های </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Escherichia coli</span></span></span></em><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> و </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Pseudomonas aeroginosa</span></span></span></em><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> اثر بازدارندگي داشت. بيش &shy;ترين اثر براي </span></span><em><span dir="LTR"><span style="font-family:b-zar,serif;"><span style="font-size:10.0pt;">S. bracteata</span></span></span></em> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">به دست آمد به&shy; طوري كه اثر بازدارندگي عصاره اين گياه براي </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Staphylococcus aureus</span></span></span></em><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">، </span></span><span dir="LTR"><span style="font-family:b-zar,serif;"><span style="font-size:10.0pt;">&micro;g/ml</span></span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> 64 و براي دو باكتري ديگر برابر با </span></span>&nbsp;<span dir="LTR"><span style="font-family:b-zar,serif;"><span style="font-size:10.0pt;">&micro;g/ml</span></span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">128 به &shy;دست آمد. </span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">عصاره </span></span><em><span dir="LTR"><span style="font-family:b-zar,serif;"><span style="font-size:10.0pt;">S. palaestina</span></span></span></em> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">اثر ضعيفي بر باکتری </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Staphylococcus aureus</span></span></span></em><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> داشت و اثر بازدارندگي در غلظت </span></span>&nbsp;<span dir="LTR"><span style="font-family:b-zar,serif;"><span style="font-size:10.0pt;">&micro;g/ml</span></span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> 1024</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">مشاهده شد. عصاره اين گونه، همچنين در غلظت </span></span><span dir="LTR"><span style="font-family:b-zar,serif;"><span style="font-size:10.0pt;">&micro;g/ml</span></span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> 512 بر باکتری &shy;های </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Escherichia coli</span></span></span></em><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> و </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Pseudomonas aeroginosa</span></span></span></em><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> اثر بازدارندگي داشت.</span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> بنابراين نتيجه &shy;گيري شد كه گونه</span></span><em><span dir="LTR"><span style="font-family:b-zar,serif;"><span style="font-size:10.0pt;">S. bracteata </span></span></span></em>&nbsp;<span style="font-family:b nazanin;"><span style="font-size:10.0pt;">مي&shy; تواند به منزله گونه &shy;اي مناسب جهت استفاده</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">ضد باکتریایی تحت مطالعه بيش تر قرار گيرد.</span></span><br /> &nbsp;</p> <p>The aim of this study was to evaluate the antibacterial activity of the water extracts of three species of <em>Salvia</em> (<em>S. perspolitana</em>, <em>S. palaestina</em>, <em>S. bracteata</em>) on <em>Staphylococcus aureus</em>, <em>Escherichia coli</em> and <em>Pseudomonas aeroginosa</em>. The antibacterial activity of water extracts of the studied species on the bacterial strains was examined using well diffusion method and minimum inhibitory concentration (MIC). Results showed that only <em>S. bracteata</em> formed growth inhibitory zone (9 mm) on <em>Staphylococcus aureus</em>. The extracts of all three plants formed growth inhibitory zone on <em>E.coli</em> and <em>P. aeroginosa</em>. The extract of <em>S. bracteata</em> was more effective than that of the other species. Results for MIC also showed that the extracts of <em>S. perspolitana</em> had the lowest effect on <em>St. aureus</em> and its MIC was observed in a concentration of 1024 &micro;g/ml. The extracts of this species had the inhibitory effect in a concentration of 256 &micro;g/ml. The uppermost inhibitory effect was provided by the extract of <em>S. bracteata</em>, since the minimum inhibitory concentration of this species for <em>S. aureus</em> was equal to 64 &micro;g/ml; and for the other two bacteria, it was equal to 128 &micro;g/ml. The extracts of <em>S. palaestina</em> had the lowest effect on <em>S. aureus</em> and its MIC was observed in a concentration of 1024 &micro;g/ml. The extracts of this species had an MIC equal to 512 &micro;g/ml for the other two bacteria. It was concluded that <em>S. bracreata</em> could be considered a suitable species with anti-bacterial activities in future researches.<br /> &nbsp;<br /> &nbsp;<br /> &nbsp;</p> ایلام, تیره نعنائیان, روش انتشار چاهک, غلظت بازدارندگی, ضد باکتری antibacteria, Ilam, inhibitory concentration, Lamiaceae, well diffusion method 446 453 http://nbr.khu.ac.ir/browse.php?a_code=A-10-346-2&slc_lang=fa&sid=1 Asghar Mosleh Arany اصغر مصلح آرانی amosleh@yazd.ac.ir 10031947532846007542 10031947532846007542 Yes Yazd University دانشگاه یزد Navid Nemati نوید نعمتی 10031947532846007543 10031947532846007543 No Yazd University دانشگاه یزد hengame Zandi هنگامه زندی 10031947532846007544 10031947532846007544 No shahid sadoughi university دانشگاه شهید صدوقی Mostafa Naderi مصطفی نادری 10031947532846007545 10031947532846007545 No ilam university دانشگاه ایلام