University of Maragheh, Maragheh, Iran , zjahanafrooz@yahoo.com
Abstract: (579 Views)
Breast cancer is the most frequently diagnosed cancer in women. Triple-negative breast cancer (TNBC) is a kind of breast cancer that does not have any of the receptors that are commonly found in breast cancer. This study aimed to evaluate differentially expressed genes (DEGs) and their related microRNAs (miRNAs) in TNBC. GSE113865 and GSE154255 were selected from GEO database. DEGs and differentially expressed miRNAs between normal and TNBC tissues were identified via GEO2R online tools and R program. STRING was used to construct a protein–protein interaction (PPI) network of DEGs. The hub genes, obtained using the cytoHubba plugin in Cytoscape. We used R program and Enrichr database to enrichment analysis of hub genes. Then intersection of predicted hub gene's miRNAs and differentially expressed miRNAs was investigated. UBE2C and SAA1 were the highest upregulated and downregulated genes, respectively. Six main hub genes were identified, including CDC20, DLGAP5, TPX2, UBE2C, TK1, and CDC45. All the mentioned genes were upregulated and enriched in cell cycle progression. Some hub genes such as TPX2, UBE2C, and TK1 were targeted by differentially expressed miRNAs (miR-3679 and miR-4530). In conclusion, knockdown of hub genes could be a targeted therapy for TNBC.
Type of Study: Original Article |
Subject:
Genetics
Received: 2023/09/12 | Revised: 2025/02/19 | Accepted: 2025/02/19
Received: 2023/09/12 | Revised: 2025/02/19 | Accepted: 2025/02/19
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