Volume 9, Issue 2 (9-2022)                   nbr 2022, 9(2): 124-131 | Back to browse issues page

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Jalali Roudsary L, Jafari A, Vaezi J, Karimi E. A simple and efficient DNA extraction protocol for old herbarium leaves of Bellevalia (Asparagaceae, Scilloideae). nbr 2022; 9 (2) :124-131
URL: http://nbr.khu.ac.ir/article-1-3520-en.html
A simple and efficient DNA extraction protocol for old herbarium leaves of Bellevalia (Asparagaceae, Scilloideae)
Lida Jalali Roudsary , Azarnoosh Jafari * , Jamil Vaezi , Ehsan Karimi
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran , azarnoosh_djafari@mshdiau.ac.ir
Abstract:   (2498 Views)
High-quality DNA extraction plays an important role to make sharp bands in the gel electrophoresis and also produces clean chromatograms. Usually, DNA extract is delivered using the modified CTAB method but this method cannot obtain high-quality DNA for molecular analysis from old dried leaves of Bellevalia due to having different chemical compounds which inhibit to obtain a clear DNA extraction. To solve the problem, in different phases of a modified CTAB protocol, activated carbon, phenol, potassium acetate, RNase and deionized water (instead of TE) were applied to eliminate contaminants and improve obtained high-quality DNA. The DNA absorption data from the modified method were compared with CTAB method in 260 and 280 nm wavelengths. The results showed a 15-80-fold change in the DNA concentration. This study suggests the modified method of extraction is more efficient in the quality of DNA obtained from the little amount of herbarium old leaves of Bellevalia than that of the CTAB method.

 
Keywords: activated charcoal, Bellevalia, DNA purification, phenol, nanodrop
Full-Text [PDF 287 kb]   (1000 Downloads)    
Type of Study: Original Article | Subject: Plant Biology
Received: 2021/12/24 | Revised: 2022/08/30 | Accepted: 2022/02/5 | Published: 2022/08/30 | ePublished: 2022/08/30
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