Volume 7, Issue 1 (4-2020)                   NBR 2020, 7(1): 9-18 | Back to browse issues page


XML Persian Abstract Print


Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran , baharara78@gmail.com
Abstract:   (2975 Views)
In traditional medicine, extracts of polysaccharide-containing plants are widely employed for the treatment of cutaneus wounds. The brittle star, as one of marine organisms, encompasses bioactive compounds, which confer the healing potency of damaged arms. The aim of the present study is to investigate the effects of extracted polysaccharide from the Persian Gulf brittle star )Ophiocoma erinaceus( on male Wistar rat skin wound healing. In this experimental study, 60 male Wistar rats were divided randomly into 5 groups including control, positive control (treatment with honey), experimental 1 to 3 (treatment with 12.5, 25, 37.5 mg/kg body weight of extracted polysaccharide, respectively). In all groups, the wound was inflicted in the posterior part of the body of rats. Then, rats were treated locally. On the 3th, 7th, 10th and 14th days, samples were collected from the healing hole and histological changes were investigated by light microscopy. Then, quantitative data were analyzed by SPSS software, one-way ANOVA at the level of p<0.05. Microscopic results showed that, in treated wounds by 37.5 mg/kg of extracted polysaccharide, as well as positive control, the re-epithelialization, the angiogenesis and the attenuation of inflammatory cells were significantly improved in comparison with the control group. Maximum epithelium thickness (on the 7th day) and minimum inflammatory cells (on the 14th day) were observed in the experimental group 3 (treated with 37.5 mg/kg of extracted polysaccharide) and positive control, as compared with the control group (p≤0.05). The findings of this research indicated that the polysaccharide extracted from O. erinaceus accelerated the cutaneus wound healing in male rats, which can be used as a natural component in the development of natural source agents for the treatment of cutaneous wounds.

 
Full-Text [PDF 1307 kb]   (918 Downloads)    
Type of Study: Original Article | Subject: Animal Biology
Received: 2018/06/7 | Revised: 2020/05/9 | Accepted: 2019/07/26 | Published: 2020/03/31 | ePublished: 2020/03/31

References
1. Alahtavakoli, M., Vazirinejad, R., Ansari, J., Negahban, T., Mashayekhi, H., Nazari, M., Ghoreshi, S. & Nematollahi, F. 2010. Effect of Teucrium polium extract on skin wound healing in rat. Hormozgan Med. J. 16: 17-24.
2. Agrawal, P., Soni, S., Mittal, G. & Bhatnagar, A. 2014. Role of polymeric biomaterials as wound healing agents. Int. J. Low Extrem. Wounds 13: 180-90. [DOI:10.1177/1534734614544523]
3. Baharara, J. & Amini, E. 2016. The potential of Brittle Star extracted polysaccharide in promoting apoptosis via intrinsic signaling pathway. Avecinna J. Med. Biotechnol. 7: 151-158.
4. Baharara, J., Mahdavi Shahri, N. & Shaddel, N. 2014. Local effect of the Persian Gulf Brittle Star (Ophiocoma erinaceus) alcoholic extract on cutaneous wound healing in Balb/C mouse. J. Birjand Uni. Med. Sci. 21: 312-23.
5. Bielefeld, K.A, Amini-Nik, S. & Alman, B.A. 2013. Cutaneous wound healing: recruiting developmental pathways for regeneration. Cell Mol. Life Sci. 70: 2059-2081. [DOI:10.1007/s00018-012-1152-9]
6. Cardose, C.R., Souza, M.A., Ferro, E.A., Favoreto, J.R., Pena, J.D. 2004. Influence of topical administration of n-3 and n-6 essential and n-9 nonessential fatty acid on the healing of cataneuos wounds. Wound Repair Regen. 12: 235- 243. [DOI:10.1111/j.1067-1927.2004.012216.x]
7. Cragg, G.M. & Newman, D.J. 2013. Natural products : A continuing source of novel drug leads. Biochim. Biophys. Acta. 830: 3670-3695. [DOI:10.1016/j.bbagen.2013.02.008]
8. Czarkwiani, A., Dylus, D.V. & Oliveri, P. 2013. Expression of skeletogenic genes during arm regeneration in the brittle star Amphiura filiformis. Gene Expr. Patterns 13: 464-72. [DOI:10.1016/j.gep.2013.09.002]
9. De Zoysa, M. 2012. Medicinal benefits of marine invertebrates: sources for discovering natural drug candidates. Adv. Food Nutr. Res. 153-169. [DOI:10.1016/B978-0-12-416003-3.00009-3]
10. Ghasemifard, S. 2011. Wound healing properties of Eucheuma cottonii extracts in Sprague-Dawley rats. J. Med. Plant. Res. 5: 6373-6380. [DOI:10.5897/JMPR10.902]
11. Hameedaldeen, A., Liu, J., Batres, A., Graves, G.S. & Graves, D.T. 2014. FOXO1, TGF-β regulation and wound healing. Int. J. Mol Sci. 15: 16257-69. [DOI:10.3390/ijms150916257]
12. Inagaki, M., Shibai, M., Isobe, R. & Higuchi, R. 2001. Constituents of ophiuroidea.1. isolation and structure of three ganglioside molecular species from the brittle star Ophiocoma scolopendrina. Chem. Pharm. Bull. 49: 1521-25. [DOI:10.1248/cpb.49.1521]
13. Kaboli, H. & Haghighat, S. 2017. Evaluation of borrago topical effects on wound healing of cutting wounds in mice. J. Shahid Sadoughi Univ. Med. Sci. 25: 311-321.
14. Lee, K.S., Shin, J.S. & Nam, K.S. 2011. Cancer chemo preventive effects of starfish polysaccharide in human breast cancer cells. Biotech. Bioprocess Eng. 16: 987-991. [DOI:10.1007/s12257-011-0226-0]
15. Li, C., Blencke, H.M., Haug, T. & Stensvåg, K. 2015. Anti-microbial peptides in Echinoderm host defense. Dev. Comp. Immunol. 49:190-197. [DOI:10.1016/j.dci.2014.11.002]
16. Miguel, D., António, G., Maria, A. & Nobre, M. 2012. Characterization of the coelomic fluid of the starfish Marthasterias glacialis in a wound-healing phase. IST 2: 1-73.
17. Mirjalili, S.M., Faramarzi, S., Esmaeilideha,j M., Zare Mehrjardi, F. & Rezvani, M.E. 2017. Effect of aqueous extract of Morus nigra on skin wound healing in type 1 diabetic rats. J. Shahid Sadoughi Univ. Med. Sci. 25: 264-70.
18. Prabhu, K. & Bragadeeswaran, S. 2013. Biological properties of brittle star Ophiocnemis marmorata collected from parangipettai, southeast coast of India. J. Microbiol. Antimicrob. 5: 110-118. [DOI:10.5897/JMA2013.0270]
19. Salama, M., Elgadir, M.A. & Adam, A. 2014. Antioxidant, Antimicrobial and Antiinflammatory effects of selected plants on wound healing. World J. Pharmacy. Pharmaceutical. Sci. 3: 1-11.
20. Silva, T.H., Alves, A., Ferreira, B.M., Oliveira, J.M., Reys, L.L. & Ferreira, R.J.F. 2012. Materials of marine origin: a review on polymers and ceramics of biomedical interest. Int. Materials Rev. 57: 276-306. [DOI:10.1179/1743280412Y.0000000002]
21. Smith, L.C., Ghosh, J., Buckley, K.M., Clow, L.A., Dheilly, N.M. & Haug, T. 2010. Echinoderm immunity. Adv. Exp. Med. Biol. 708: 260-271. [DOI:10.1007/978-1-4419-8059-5_14]
22. Soheili, A., Baharara, J., Mahdavi Shahri, N., Zafar Balanejad, S. & Amini, E. 2016. Protective Effect of the Persian Gulf brittle star Ophiocoma erinaceus extract on carbon tetrachloride (CCl4) induced liver damage in adult male Wistar rat. J. Birjand Uni. Med. Sci. 22: 316-326.
23. Sohrabifar, N., Baharara, J., Mahdavi Shahri, N., Zafar Balanejad, S. & Amini, E. 2016. The impact of sheep testis extract on hair follicle growth and skin healing of Wistar rats. Nova Biol. Reperta 3: 24-38. [DOI:10.21859/acadpub.nbr.3.1.24]
24. Suntar, I., Tatli, I., Kupeli Akkol, E. & Keles, H. 2010. An ethnopharmaclogical study on Verbascum species: from conventional wound healing use to scientific verification. J. Ethnopharmacol. 132: 8-13. [DOI:10.1016/j.jep.2010.08.004]
25. Zueva, O., Khoury, M., Heinzeller, T., Mashanova, D. & Mashano, V. 2018. The complex simplicity of the brittle star nervous system. Front. Zool. 15:1-26. [DOI:10.1186/s12983-017-0247-4]

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.