Volume 5, Issue 3 (12-2018)                   nbr 2018, 5(3): 237-243 | Back to browse issues page

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khaleqsefat E, Khalaj-Kondori M, Jabbarpour bonyadi M, Soraya H, Askari B. Frequency of two VKORC1 gene variants and its correlation with warfarin maintenance dose. nbr. 2018; 5 (3) :237-243
URL: http://nbr.khu.ac.ir/article-1-3124-en.html
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
Abstract:   (1088 Views)
Warfarin is a commonly-prescribed anticoagulant used to treat and prevent thromboembolic events. The requirement for varying doses of warfarin depends on genetic and environmental components. In this study, the frequency of two single-nucleotide polymorphic variants of the vitamin K epoxide reductase complex subunit 1 (VKORC1) gene (1173 C>T (rs9934438) and 3730 G>A (rs7294)) and its correlation with warfarin maintenance doses were investigated in patients with heart valve replacement from West Azarbayejan, Iran. Blood samples were obtained from 185 patients; their genomic DNA was extracted and samples were genotyped by polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) assay. To assess if the blood warfarin level is different among genotypes, we used a one-way analysis of variance (ANOVA) followed by a Tukey’s post-hoc comparison. The minor allele frequency was determined to be 54% for 1173T and 53.7% for 3730A. Patients who carried the G allele at position 3730 and T allele at position 1173 required a significantly lower daily mean warfarin dosage (P <0.001). Consideration of the VKORC1 gene polymorphism, especially at the initial stages of the therapy, can be helpful in pre-treatment dosing of warfarin, which, in turn, reduces the adverse effects resulting from inappropriate drug prescription.                                                                                                                                                           

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Type of Study: Original Article | Subject: Genetics
Received: 2018/04/12 | Revised: 2019/02/11 | Accepted: 2018/08/2 | Published: 2018/12/21 | ePublished: 2018/12/21

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