Volume 4, Issue 4 (3-2018)                   nbr 2018, 4(4): 299-309 | Back to browse issues page

DOI: 10.29252/nbr.4.4.299

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University of Zabol
Abstract:   (2625 Views)
Phenolic compounds, especially those with plant origin, constitute an essential part of the human diet due to their antioxidant properties. Antioxidants protect biological systems (nucleic acids, proteins, lipids, lipoproteins) against free radicals and reactive oxygen species. Some medicinal plants contain so high amounts of antioxidants that their use can be considerably effective for human health. The aim of this study was to evaluate the effect of flooding extraction method on phenolic compounds, flavonoids and the level of free radical scavenging properties of medicinal plants including Thyme, Hamadani Yarrow, Shirazi mountain thyme, Yarrow, Chamomile, Saffron corm hyssop Sagebrush, wormwood, Artemisia and Ziziphora clinopodioiedes, in four extraction solvents including water, methanol, acetone and ethanol. The results showed that methanol and water solvents had the highest and the lowest total phenolic, flavonoid and antioxidant activity, respectively. The results also showed that methanol extraction of Shirazi thyme and water extraction of Wormwood plant had the highest and the lowest phenol and flavonoids contents, respectively. The results of trapping the free radicals of DPPH indicated that the methanol extract of Shirazi thyme and aqueous extract of wormwood had the highest and the lowest inhibition activities, respectively. Therefore, the selection of solvent type can affect the rate of the extraction of phenolic compounds, flavonoids and antioxidant activity.
Full-Text [PDF 636 kb]   (2410 Downloads)    
Type of Study: Original Article | Subject: Plant Biology
Received: 2017/02/11 | Revised: 2018/05/9 | Accepted: 2017/12/31 | Published: 2018/03/19 | ePublished: 2018/03/19

1. Abbas Vali, M., Esmaeili Kutmehr, M., Moshtaghi, H. and Escanderi, M.H. 2016. Evaluation of antibacterial extracts of acetone, ethanol and methanol leave four varieties of oleo in Iranian on the E. coli. – J. Food Microbio. 2: 67-77.
2. Ahmadi, F., Kadivar, M. and Shahedi, M. 2007. Antioxidant activity of Kelussia odoratissima Moza. in model and food systems. – J. Food Chem. 105: 57-64. [DOI:10.1016/j.foodchem.2007.03.056]
3. Alam Hollo, M. and Nazeri, S. 2015. Assessments of antioxidant and antibacterial activity of extracts alcoholic of flower and root Dendrostellera lesserti on some human pathogenic bacteria. – J. Med Sci. 21: 277-285.
4. Alamhulu, M. and Nazeri, S. 2015. Investigation antibacterial and antioxidant activities of alcoholic extracts of flower and root Dendrostellera lesserti on some human pathogenic bacteria. – Sci. J. Hamadan Univ. Med. Sci. 21: 277-285.
5. Arabshahi, D.S. and Urooj, A. 2006. Antioxidant prop-erties of various solvent extracts of mulberry (Morus indica L.) leaves. – J. Food Chem. 102: 1233-1240. [DOI:10.1016/j.foodchem.2006.07.013]
6. Bahrami-Karkevandi, M., Moshtaghian, S.J., Mahzoni, P., Adibi, S. and Kazemi, S. 2011. The effects of hydroalcoholic extract of Artemisia aucheri on bleo-mycin induced pulmonary fibrosis in rats. – J. Med. Sci. 12: 33-40.
7. Betancourt, A.O. 2008. Analysis, extraction and recovery of poly-3- hydroxybutyrate in the biomass. – Univ-ersity of Quebec at Montreal Thesis, pp 45-55.
8. Burits, M. and Bucar, F. 2000. Antioxidant activity of Nigella sativa essential oil. – Phyto. Res.14: 323-328. https://doi.org/10.1002/1099-1573(200008)14:5<323::AID-PTR621>3.0.CO;2-Q [DOI:10.1002/1099-1573(200008)14:53.0.CO;2-Q]
9. Chang, Y.L., Kim, D.O., Lee, K.W., Lee, H.J., Lee, C.Y. 2002. Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals. – J. of Agri. and Food Chem. 50: 3713-3717. [DOI:10.1021/jf020071c]
10. Choe, E. and Min, B.D. 2009. Mechanisms of antioxidants in the oxidation of food. – Rev. in Food Sci. and Food Safety 8: 345-358. [DOI:10.1111/j.1541-4337.2009.00085.x]
11. Ebrahimzadeh, M.A., Hosseinimehr, S J., Hamidinia, A. and Jafari, M. 2008. Antioxidant, and free radical scavenging activity of Feijoa sallowiana fruits peel and leaves. – Pharmacology 1: 7-14.
12. Fallah, H., Ksouri, R., Lucchessi, M.E., Abdelly, C.H. and Magné, C.H. 2012. Ultrasound-assisted extraction: effect of exraction time and solvent power onvthe levels of polyphenols and antioxidant activity of Mesembryanthemum edule L. Aizoaceae shoots. – Tro. J. of Pharm. Res. 11: 243-249. [DOI:10.4314/tjpr.v11i2.10]
13. Gharehkhani, M., Ghorbani, M., Ebrahimzadeh, M.A., Gafari, S.M. and Sadeghimahonak, A.R. 2012. Comparison of different methods of extraction of phenolic compounds and flavonoids of Urtica dioica L. – Quarterly Sci. Res. of Med. and Aromatic Plants. 26: 389-405.
14. Gliszczynska-Swigło, A. 2006. Antioxidant activity of water soluble vitamins in the TEAC (trolox equivalent antioxidant capacity) and the FRAP (ferric reducing antioxidant power) assays. – J. Food Chem. 96: 131-136. [DOI:10.1016/j.foodchem.2005.02.018]
15. Hakkinen, S.H. and Torronen A.R. 2000. Content of flavonols and selected phenolic acids in Strawberries and Vaccinium species: influence of cultivar, culti-vation site and technique. – Food Res. Int. 33: 517-524. [DOI:10.1016/S0963-9969(00)00086-7]
16. Hayouni, A., Abedrabba, M., Bouix, M. and Hamdi, M. 2007. The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera and Juniperus phoenicea fruit extracts. – J. Food Chem. 105: 1126-1134. [DOI:10.1016/j.foodchem.2007.02.010]
17. Hwang, J.Y., Shue, Y.S. and Chang, H.M. 2001. Antioxidative activity of roasted and defatted peanut kernels. – Food Res. Int. 34: 639-647 [DOI:10.1016/S0963-9969(01)00083-7]
18. Kamkar, A., Jebelli Javan, A., Asadi, F. and Kamalinejad, M. 2010. The antioxidative effect of Iranian Mentha pulegium extracts and essential oil in sunflower oil. – Food and Chem. Toxicol. 48: 1796-1800. [DOI:10.1016/j.fct.2010.04.003]
19. Karaman, I., Sahin, F. and Gullule, M. 2003. Antimicrobial activity of aqueous and methanol extraets of Juniperus oxycedrus. – J. Ethnophar-macol. 85: 231-235. [DOI:10.1016/S0378-8741(03)00006-0]
20. Khalili, M. and Ebrahimzadeh, M.A.A. 2015. Review on antioxidants and some of their common evaluation methods. – J. Mazandaran Univ. Med. Sci. 24: 188-208.
21. Lee, K.W., Kim, Y.J., Kim, D.O., Lee, H.J. and Lee, C.Y. 2003. Major phenolics in apple and their contribution to the total antioxidant capacity. – J. Agric. Food Chem. 51: 651-20. [DOI:10.1021/jf034475w]
22. Lee, S.E., Hwang, H.J., Ha, J.S., Jeong, H.S. and Kim, J. H. 2003. Screening of medicinal plant extracts for antioxidant activity. – Life Sci. 73: 167-179. [DOI:10.1016/S0024-3205(03)00259-5]
23. Lutza, M., Henrı´quez, C. and Escobar, M. 2011. Chemical composition and antioxidant properties of mature and baby artichokes (Cynara scolymus), raw and cooked. – J. Food Compos. Anal. 24: 49-54. [DOI:10.1016/j.jfca.2010.06.001]
24. Mahdavi, D.L., Deshpande, S.S. and Salunkhe, D.K. 1995. Food antioxidant. – Marcel Dekker Inc., New York, USA, 746 pp.
25. Mariken, J.T.J., Sebastiaan Dallinga, J., Hans-Peter, V., Guido, R.M.M. and Aalt, B. 2003. A critical appraisal of the use of the antioxidant capacity (TEAC) assay in defining optimal antioxidant structures. – Food Chem. 80: 409-414. [DOI:10.1016/S0308-8146(02)00468-5]
26. Mazandarani, M., Makri, S. and Bajian, G.R. 2011. Evaluation of phytochemical and antioxidant activity in different parts of Heracleum gorganicum Rech. f. in Golestan Province, north of Iran. – J. Plant Physio. 2: 381-388.
27. Mirzaei A, Akbartabartori M, Sadeghi H, Sharifi B. 2010. The evaluation of total phenol and antioxidant activity yarrow, wormwood and chamomile. – Journ-al of Armaghane Danesh. 15: 243-252.
28. Morello, J.R, Romero, M.P., Ramo, T. and Motilva, M. J. 2005. Evaluation of 1-phenylalanine ammonia-lyase activity and phenolic profile in olive drupe (Olea europaea) from fruit setting period to harvesting time. – Plant Sci. 168: 65-72. [DOI:10.1016/j.plantsci.2004.07.013]
29. Mour, A., Sruz, G.M., Franco, D. and Dominguez, J. 2001. Natural antioxidant from residual sources – Food Chem. 72: 145-171. [DOI:10.1016/S0308-8146(00)00223-5]
30. Ordone, A.A.L., Gomez, J.D. and Vattuone, M.A. 2008. Antioxidant activities of Sechium edule Swartz extracts. – Food Chem. 97: 452-458. [DOI:10.1016/j.foodchem.2005.05.024]
31. Piluzza, G. and Bullitta, S. 2011. Correlations between phenolic content and antioxidant properties in twenty-four plant species of traditional ethnoveteri-nary use in the Mediterranean area. – Pharm. Biol. 49: 240-247. [DOI:10.3109/13880209.2010.501083]
32. Rahimi, M. and Ramezani, M. 2017. The effects of temperature on antioxidant activity, total phenolics and agronomic traits of two thyme species. – Nova Biologica Reperta. 4: 264-270.
33. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. 1998. Antioxidant activity applying an improved antiradical action decolonization assay. – PII S0891-5849, 00315-3
34. Sadeghi, A.R., Salmaneian, Sh., Jamsun, M. and Tabatabai Amid, B. 2014. Identify and measure the phenolic acids, radical scavenging activity and reducing power iron methanol extracts and ethanol Eryngium caucasicum. – J. Res. and Innovation in Food Sci. and Tech. 2: 193-204
35. Samsam Shariat, Sh. 1993. Extraction of effective components of herbal medicine, determination and evaluation methods. – Mani Press. Esfahan. Iran. pp: 12 -13.
36. Serrano, J., Go-i, I. and Saura-Calixto, F. 2006. Food antioxidant capacity determined by chemical methods may underestimate the physiological antioxidant capacity. – Food Rev. Int. 40: 15-21. [DOI:10.1016/j.foodres.2006.07.010]
37. Shi, J., Nawaz, H., Pohorly, J. and Mittal, G. 2005. Extraction of polyphenolics from plant material for functional foods engineering and technology. – Food Rev. Int. 21: 1-12. [DOI:10.1081/FRI-200040606]
38. Siriamompus, S. and Suttajit, M. 2010. Microchemicals compounds and antioxidant activity of different morphological part of thai wildpurslane (Portulaca oleracea). – Weed Sci. 58: 182-188. [DOI:10.1614/WS-D-09-00073.1]
39. Thi, N.D. and Hwang, E.S. 2014. Bioactive compound contents and antioxidant activity in Aronia (Aronia melanocarpa) leaves collected at different growth stages. – Prev. Nutr. Food Sci. 19: 204-212. [DOI:10.3746/pnf.2014.19.3.204]
40. Vilkha, K., Mawson, R., Simons, L. and Bates, D. 2008. Application and opportunities for untrasound assisted extraction in food industry; a review. – Innov. Food Sci. and Emer. Tech. 9: 161-169. [DOI:10.1016/j.ifset.2007.04.014]
41. Zgoda, L.R. and Porter, J.R.A. 2001. Convenient microdultion method for screening natural products against bacteria and fungi – Pharmaceut. Biol. 39: 54-221. [DOI:10.1076/phbi.]