Volume 5, Issue 4 (3-2019)
nbr 2019, 5(4): 420-427 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
ٍEbrahimi M, Kiarostami K, Nazem Bokaee Z. Effect of salicylic acid on antioxidant properties of In vitro proliferated shoots of Melissa officinalis L.. nbr 2019; 5 (4) :420-427
URL: http://nbr.khu.ac.ir/article-1-2705-en.html
URL: http://nbr.khu.ac.ir/article-1-2705-en.html
Alzahra University, , su_kiarostami@yahoo.com
Abstract: (5959 Views)
Melissa officinalis is a medicinal plant belong to lamiacea family. This plant has been cultivated in many parts of the world due to its therapeutic effects. This study was conducted to improve antioxidant activities of proliferated shoots from tissue culture. The seeds of plant were cultured in MS hormone free medium in order to obtain seedlings. Then the shoots were transfer to MS medium supplemented with 2 mg/ L BAP.After 45 days salicylic acid (SA) was added to the medium at concentrations of 0, 50, 100 and 200 µM. The antioxidant activity and phenolics, flavonoids and rosmarinic acid content in proliferated shoots were evaluated 4,7,10 and 14 days after treatment. As a result the highest free radical scavenging and reducing power activity, as well as the highest value of total phenolic and rosmarinic acid were observed in shoots after 14 days of treatment with 100 µM salicylic acid. The elevated level of superoxide anion scavenging activity and total flavonoids were obtained from the shoots treated with 50 µM salicylic acid for 14 days. Over all treatment with100 µM salicylic acid for 14 days was better in order to antioxidant activity and compounds with recognized antioxidant activity.
Type of Study: Original Article |
Subject:
Plant Biology
Received: 2016/12/24 | Revised: 2021/06/1 | Accepted: 2018/03/11 | Published: 2019/03/18 | ePublished: 2019/03/18
Received: 2016/12/24 | Revised: 2021/06/1 | Accepted: 2018/03/11 | Published: 2019/03/18 | ePublished: 2019/03/18
References
1. Brandão, I.R., Kleinowski, A.M., Einhardt, A.M., Lima, M.C., Amarante, L.D., Peters, J.A. and Braga, E.J.B. 2014. Salicylic acid on antioxidant activity and betacyanin production from leaves of Alternanthera tenella. – Ciênc. Rural. 44: 1893-1898 [DOI:10.1590/0103-8478cr20130873]
2. Buitelaar, R.M., Casário, M.T. and Tramper, J. 1992. Elicitation of thiophene production by hairy roots of Tagetespatula. – Enzy. Microbiol. Technol. 14: 2-7. [DOI:10.1016/0141-0229(92)90017-I]
3. Chen, Z., Silva, S. and Klessig, D.F. 1993. Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid. – Science 262: 1883-1886. [DOI:10.1126/science.8266079]
4. Conde, E., Cadahia, E. and Garcia-Vallejo, M.C. 1995. HPLC analysis of flavonoids and phenolic acids and aldehydes in Eucalyptus spp. – Chromatographia 41:657-660. [DOI:10.1007/BF02267800]
5. Dogbo, D.O., Gogbeu, S.J., N'zue., B., Yao, K.A., Zohouri, G.P., Mamyrbekova-Bekro, J.A. and Bekro Y.A. 2012. Comparative activities of phenylalanine ammonia-lyase and tyrosine ammonia-lyase and phenolic compounds accumulated in Cassava elicited cell. – Afri. Crop. Sci. J. 20: 85-94.
6. Ebrahimi, M., Kiarostami, Kh.and Nazem Bokaee, Z. 2015. The effect of different concentrations of kinetin on Melissa officinalis micropropagation and rosmarinic acid content in tissue culture. 18th National and 6th International Congress of Biology, Tehran. pp: 26-29.
7. Fletcher, R.S., Slimmon, T., McAuley. C.Y and Kott, L. 2005. Heat stress reduces the accumulation of rosmarinic acid and the total antioxidant capacity in spearmint (Mentha spicata L.). – Sci. Food . Agri. 85: 2429-2436.
8. Gorbani, N., Moradi, H., Akbarpour, A. and Ghasem-nezhad, A. 2013. The phytochemical changes of violet flowers (Viola cornuta) response to exogenous salicylic acid hormone. – JCHR. 3:1-8.
9. Hayat, S., Hasan, S.A., Fariduddin, Q. and Ahmad, A. 2008. Growth of tomato (Lycopersicon esculentum) in response to salicylic acid under water stress. – Int. J. Plant. Sci. 3: 297-304.
10. Jiao. Z, Liu, J. and Wang, S. 2005. Antioxidant activity of total pigment extract from Blackberries – Food. Technol. Biotech. 43 : 97-102.
11. Kiarostami, Kh., abdolmaleki, N. and Heydari, M. 2013. Effect of salicylic acid on salinity stress reduction in canola. – Plant Biol. 4: 69- 82.
12. Kulisic-Bilusic, T., Katalinic,V., Dragovic-Uzelac,V., Ljubenkov, I., Krisco, A., Dejannovic, B. and Jukic, M. 2008. Antioxidant and acetylcholinesterase inhibiting activity of several aqueous tea infusions in vitro – Food. Technol. Biotech. 46: 368-375.
13. Khaki, A., Imani, S.A.M. and Golzar, S. 2012. Effects of rosmarinic acid on male sex hormones (testosterone-FSH-LH) and testis tissue apoptosis after exposure to electromagnetic field (EMF) in rats. – Afr. J. Pharm. Pharmaco. 6: 248-252.
14. Lee, S.E., Hwang, H.J., Ha, J.S., Jeong, H.S. and Kim, J.H. 2003. Screening of medicinal plant extracts for antioxidant activity. – Life. Sci. 73: 167-179. [DOI:10.1016/S0024-3205(03)00259-5]
15. Lopez-Arnaldos, T., Lopez-Serrano, M., Ros., Barcelo, A., Calderon, A.A. and Zapata, J.M. 1995. Spectroph-otometric determination of rosmarinic acid in plant cell cultures by complexation with Fe2+ ions. – Fresen. J. Anal. Chem.- 351: 311-314. [DOI:10.1007/BF00321655]
16. López Arnaldos, T., Mu-oz, R., Ferrer, M.A and Calderón, A.A. 2001 .Change in phenol content during straberry callus. – Plant. Physiol. 113:315-322. [DOI:10.1034/j.1399-3054.2001.1130303.x]
17. Matkowski, A. 2008. Plant in vitro culture for the production of antioxidants. – Biotechnol. Adv. 26: 548-560. [DOI:10.1016/j.biotechadv.2008.07.001]
18. Meftahizade, H., Moradkhani, H., Naseri, B., Lotfi, M. and Naseri, A. 2010. Improved in vitro culture and micropropagation of different Melissa officinalis L. genotypes. – J. Med. Plants. Res. 4: 240-246.
19. Meftahizade, M., Lotfi, M. and Moradkhani, H. 2012. Optimization of micropropagation and establishment of cell suspension culture in Melissa officinalis L. – Afr. J. Biotechnol. 9: 4314-4321.
20. Mohebalipour, N., Aharizad, S., Mohammadi, S., Motallebiazar, A. and Arefi, H.M. 2012 . Effect of. plant growth regulators BAP and IAA on micropropagation of Iranian lemon balm (Melissa officinalis L.) landraces. – J. Food. Agric. Environ. 10: 280-286.
21. Mohamadi, S., Kiarostami, Kh.and Nazem Bokaii, Z. 2014. The Study of antioxidant property of metanolic extracts of Melissa officinalis L. and Salvia officinalis L. on stability of soybean oil. – J. Agroaliment. Proc. Technol. 20: 293-297.
22. Molyneux, P. 2004. The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating anti-oxidant activity. – J. Sci. Technol. 26: 211-219.
23. Namdeo, A.G. 2007. Plant cell elicitation for production of secondary metabolites-A review. – Pharm. Rev. 1: 69-79.
24. Niciforovic, N., Mihailovic, V., Maskovic, P., Solujic,S., Stojkovic, A. and PavlovicMuratspahic, D. 2010. Antioxidant activity of selected plant species; potential new sources of natural antioxidants. – Food Chem. Toxicol. 48: 3125-3130. [DOI:10.1016/j.fct.2010.08.007]
25. Nicholson, R.L. and Hammerschmidt, R. 1992. Phenolic compounds and their role in disease resistance. – Annu. Rev. Phytopathol. 30: 369-389. [DOI:10.1146/annurev.py.30.090192.002101]
26. Nurgroho, L.H., Verberne, M.C and Verpoorte, R. 2002 .Activities of enzymes involved in the phenylpropanoid pathway in constitutively salicylic acid-producing tobacco plants. – Plant Physiol. Bioch. 40: 755-760. [DOI:10.1016/S0981-9428(02)01437-7]
27. Ozturk, M.E., Duru, M., Burco, I., Harmandar, M. and Topcu, G. 2010. A new rapid spectrophotometric method to determine the rosmarinic acid level in plant extracts. – Food. Chem. 123. 1352-1356. [DOI:10.1016/j.foodchem.2010.06.021]
28. Ondrejovic. M., Kraic. F., Benkovicova, H. and Silhar, S. 2012. Optimization of antioxidant extraction from Lemon Balm (Melissa officinalis). – Czech. J. Food. Sci. 30: 385-393. [DOI:10.17221/436/2010-CJFS]
29. Pancheva, T.V., Popova, L.P. and Uzunova, A.N. 1996. Effect of salicylic acid on growth and photosynthesis in barley plants. – J. Plant. Physiol. 149: 57-63. [DOI:10.1016/S0176-1617(96)80173-8]
30. Peeva, G., Penchevb, P., Pesheva, D. and Angelovb, G. 2011. Solvent extraction of rosmarinic acid from lemon balm and concentration of extracts by nanofiltration: Effect of plant pre-treatment by supercritical carbon dioxide. – Chem. Eng. Res. Des. 89: 2236-2243. [DOI:10.1016/j.cherd.2011.04.014]
31. Raskin, I. 1992. Role of salicylic acid in plants. – Annu. Rev. Plant Biol. 43: 439-463. [DOI:10.1146/annurev.pp.43.060192.002255]
32. Raghavendra, H., Vijayananda, B., Madhumathi,G. and Hiremath, A. 2010.In vitro antioxidant activity of Vitexnegundo L. Leaf extracts. – Chiang Mai. J. Sci. 37: 489-497.
33. Robak, J. and Gryglewski, R.J. 1988. Flavonoids are scavengers of superoxide anions. – Biochem. Pharmacol. 37: 837-841. [DOI:10.1016/0006-2952(88)90169-4]
34. Rowshan, V., Khosh Khoi, M. and Javidnia, K. 2010. Effects of salicylic acid on quality and quantity of essential oil components in Salvia macrosiphon. – J. Biol. Environ. Sci. 4: 77-82.
35. Sakhanokho, H.F. and Kelley, R.Y. 2009. Influence of salicylic acid on in vitro propagation and salt tolerance in Hibiscus acetosella and Hibiscus moscheutos (cv 'Luna Red'). – Afr. J. Biotechnol. 8: 1474-1481.
36. Shabrangi, A. and BeigiJazi, E.A. 2014. Effect of salicylic acid on the amount of essential oil, phenolic compo-unds, flavonoids and antioxidant activity of Mentha piperita L. – Int. J. Agric. Crop. Sci. 7-8: 499-502.
37. Shahadat Hossan, M.D., Shiblur Rahman, A.B.M., Anwarul Bashar, A.B.M. and Rahmatollah, M. 2014. Rosmarinic acid :A review of its anticancer. – W. J. Pharm. Pharmace. Sci. 3: 57-70
38. Weitzel, C. and Petersen, M. 2011. Cloning and characterisation of rosmarinic acid synthase from Melissa officinalis L. – Phytochem. 72 : 572-578. [DOI:10.1016/j.phytochem.2011.01.039]
39. Wang, Z., Ma, L., Zhang, X., Xu, L., Cao, J. and Jiang, W. 2015. The effect of exogenous salicylic acid on antioxidant activity, bioactive compounds and antioxidant system in apricot fruit. – Scientia. Hort. 181: 113-120. [DOI:10.1016/j.scienta.2014.10.055]
40. Wen, P.F., Chen, J.Y., Wan, S.B., Kong, W.F., Zhang, P., Wang, W., Zhan, J.C., Pan, Q.H. and Huang, W.D. 2008. Salicylic acid activates phenylalanine ammonia lyase in grape berry in response to high temperature stress. – Plant. G. Regu 55: 1-10. [DOI:10.1007/s10725-007-9250-7]
41. Yosofi, M., Hojjati, M.R., Moshtaghi, E., Rahimiyan, R., Dawodiyan Dehkordi, A. and Rafieian, M. 2011. The effect of hydro-alcoholic extract of Melissa officinalis on learning and spatial memory in Balb/c mice. – J. Shahrekord. Uni. Med. Sci. 13: 51-59.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
© 2024 CC BY-NC 4.0 | Nova Biologica Reperta
Designed & Developed by : Yektaweb