بررسی اثر رویشگاه بر میزان خواص ضدمیکربی گیاه سگ دندان خاردار (Pycnocycla spinosa Decne.) در ایران

نویسندگان

1 دانشگاه دامغان

2 دانشگاه قم

چکیده
در زندگی امروزه شناسایی و معرفی گیاهان دارویی از اهمیت ویژه ای برخوردار است. جنس Pycnocycla متعلق به خانواده چتریان است که در ایران دارای 8 گونه بومی است. گونه Pycnocycla spinosa (سگ دندان خاردار) به دلیل داشتن اثرات دارویی همواره قابل توجه بوده است. هدف از این پژوهش بررسی اثر سه رویشگاه (دامغان، سمنان و کرمان) بر روی خواص ضدمیکروبی عصاره‌های استخراج شده از گیاه سگ دندان خاردار با استفاده از حلال‌های مختلف می‌باشد. برای بررسی خواص ضدمیکروبی از غلظت mg/ml 2 عصاره‌های متانولی، اتیل استاتی و هگزانی و روش انتشار دیسک در آگار بر علیه دو باکتری بیماری‌زا گرم منفی یرسینیا انتروکولیتیکا و سالمونلا انتریکا استفاده شد. نتایج نشان داد که عصاره‌های تهیه شده اثرات متفاوتی بر روی باکتری‌ها داشتند. همچنین مناطق مختلف جغرافیایی ایران، به دلیل شرایط اقلیمی متفاوت، دارای اثرات متفاوتی بر باکتری‌ها بودند. عصاره‌های تهیه شده از کرمان، به‌ویژه با حلال متانول و اتیل استات، بیشترین فعالیت ضد میکروبی را علیه باکتری سالمونلا انتریکا نشان دادند. بنابراین نوع حلال و نوع رویشگاه می‌تواند تاثیر بسزایی بر روی خواص ضد میکروبی گیاهان داشته باشد.

کلیدواژه‌ها


عنوان مقاله English

Investigation of the habitat effect on the antimicrobial properties of Pycnocycla spinosa (Decne.) in Iran

نویسندگان English

Afsaneh Teymouri 1
Atefe Amirahmadi 1
Reza Naderi 1
Fatemeh Salimi 1
Reza Sheikhakbari-Mehr 2
1 Damghan University
2 University of Qom
چکیده English

The identification and introduction of medicinal plants hold significant importance in modern life. The genus Pycnocycla, belonging to the Apiaceae family, includes eight endemic species in Iran. P. spinosa has attracted attention due to its medicinal properties. This study aims to investigate the effect of three habitats (the Damghan, Semnan, and Kerman city) on the antimicrobial properties of extracts obtained from Pycnocycla spinosa using different solvents. To examine the antimicrobial properties, methanolic, ethyl acetate, and hexane extracts at a concentration of 2 mg/ml were tested against two pathogenic Gram-negative bacteria, Yersinia enterocolitica and Salmonella enterica, using the agar disk diffusion method. The results indicated that the extracts had varying effects on the bacteria. Additionally, the different geographical regions of Iran, due to their distinct climatic conditions, exhibited varying effects on the bacteria. Extracts from Kerman, especially those using methanol and ethyl acetate as solvents, showed the highest antimicrobial activity against Salmonella enterica. Therefore, the type of solvent and habitat can significantly influence the antimicrobial properties of plants.

کلیدواژه‌ها English

Iran
Pycnocycla spinosa
pathogenic bacteria
traditional medicine
فهرست منابع
Adhikari, P., Joshi, K., Singh, M., Pandey, A. 2022. Influence of altitude on secondary metabolites, antioxidants, and antimicrobial activities of Himalayan yew (Taxus wallichiana). Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology. 156(1): 187-195.
Ahmadi, L., & Mirza, M. 1998. Volatile Constituents of the Essential Oil of Pycnocycla spinosa Decne. & Boiss. from Iran. Journal of Essential Oil Research, 10(2), 197–198. https://doi.org/10.1080/10412905.1998.9700878
Arzi, A., Azemi, E., Sistani Karampour, N., Fakhri Behbahani, A. 2017. Evaluation of Analgesic Effect of the Hydro-alcoholic Extract of Pycnocycla caespitosa in Comparison with Morphine and Aspirin by Formalin Test. Jundishapur Scientific Medical Journal,16(2): 143-151. doi: 10.22118/jsmj.2017.49239
Asgarpanah, J., Darabi-Mahboub, E., Mahboubi, A., Mehrab, R., Hakemivala, M. 2013. In-Vitro evaluation of Crocus sativus L. petals and stamens as natural antibacterial agents against food-borne bacterial strains. Iranian Journal of Pharmaceutical Sciences. 9(4):69-82.
Askari, F., Sefidkon, F., Soltanipour, M., Mirza, M., Fekri Qomi, S. 2022. Chemical compounds of essential oil in Pycnocycla flabellifolia Boiss. and P. nodiflora Decne ex. Boiss. In Iran. Iranian Journal of Medicinal and Aromatic Plants Research, 38(2): 191-199. doi: 10.22092/ijmapr.2021.352261.2884
Asghari, G., Houshfar, G., Mahmoudi, Z. 2001. Composition of the essential oil of Pycnocycla spinosa Decne. ex Boiss. Journal of pharmaceutical sciences. 9:28-29.
Asghari, Gh. R., Lajvardi, S.M. 2013. Determination of sucrose content in Pycnocycla spinosa Decne. Ex Bioss. root using polarimetry. Journal of Sugar Beet. 29:13-15.
Chrysargyris, A. Mikallou, M., Petropoulos, S., Tzortzakis, N. 2020. Profiling of essential oils components and polyphenols for their antioxidant activity of medicinal and aromatic plants grown in different environmental conditions. Agronomy. 10(5): 727. https://doi.org/10.3390/agronomy10050727
Clarkson, J.J., Zuntini, A.R., Maurin, O., Downie, S.R., Plunkett, G.M., Nicolas, A.N., Smith, J.F., Feist, M.A.E., Gutierrez, K., Malakasi, P. and Bailey, P. 2021. A higher‐level nuclear phylogenomic study of the carrot family (Apiaceae). American Journal of Botany, 108(7): 1252-1269.
Elkady, W.M.; Gonaid, M.H.; Yousif, M.F.; El-Sayed, M.; Omar, H.A.N. 2021. Impact of altitudinal variation on the phytochemical profile, anthelmintic and antimicrobial activity of two Pinus species. Molecules 26: 3170. https://doi.org/10.3390/molecules26113170
Hosseaini, R. S., Asgarpanah, J., Bakhtiarian, A., Mousavi, Z. 2022. 'Phytochemical Analysis, Antinociceptive and Anti-Inflammatory Effects of Pycnocycla Bashgardiana Aerial Parts Essential Oil in Experimental Animals. Journal of Medicinal plants and By-Products, 11(2): 231-238. doi: 10.22092/jmpb.2021.343038.1213.
Jalali, M., Abedi, D., Asghari, G.R., Rezaie, Z. 2007. A study of anti-microbial effect of Pycnocycla spinosa's fruit extracts. Journal of Mazandaran University Medical Sciences. 17(59): 76-86. URL: http://jmums.mazums.ac.ir/article-1-235-en.html
Khalil, N., El-Jalel, L., Yousif, M. 2020. Altitude impact on the chemical profile and biological activities of Satureja thymbra L. essential oil. BMC Complementary Medicine and Therapies 20: 186. https://doi.org/10.1186/s12906-020-02982-9
Mahboubi, M., Mahdizadeh, E., Heidary Tabar R. 2016. The chemical composition, antimicrobial, and antioxidant activities of Pycnocycla spinosa and Pycnocycla flabellifolia essential oils. Zeitschrift fur
Mahboubi, M., Taghizadeh, M., & Kazempour, N. 2014. Antimicrobial and antioxidant activities of Pycnocycla spinosa extracts. Jundishapur Journal of Natural Pharmaceutical Products, 9(3): e13859. doi: https://doi.org/10.17795/jjnpp-13859
Mirzaei, A., Akbartabar, M., Sadeghi, H., Sharifi, B. 2010. The Antioxidant activities and total phenolic of Artemisia martima, Achillea millefolium and Matricaria recutica. Journal of Armaghan-e Danesh.15(1):243-252.
Mohebbizadeh, S.H., Naderi, Gh. 2024. The effects of changes in altitude on quantity of essential oil, carvacrol and thymol of Thymus kotschyanus Boiss in Central Province (Arak). Journal of Medicinal Plants and Herbs. 3:203.
Mozaffarian, V. 2007: Umbelliferae. In: Flora of Iran (eds. Assadi, M., Khatamsaz, M., Maassoumi, A. A.) No. 54. -Tehran (in Persian).
Naturforschung - Section C Journal of Biosciences. 71(11-12): 403-408. doi:10.1515/znc-2016-0038.
Okmen, G., Kardas, S., Bayrak, D., Arslan, A., Cakar, H. 2016. The antibacterial activities of Crocus sativus against mastitis pathogens and its antioxidant activities. World Journal of Pharmacy and Pharmaceutical Sciences.5(3)146-156.
Sadraei, H., Asghari, G., Arabzadeh, A. 2004. Effect of hydroalcoholic extract of Pycnocycla spinosa on rat isolated bladder contraction. Iranian Journal of Pharmaceutical Research. 4:237–41.
Sadraei, H., Asghari, G., Hajhashemi, V., Nezami, M. 2006. Evaluation of cardiovascular effect of Pycnocycla spinosa Decne. Ex Boiss. var. spinosa extract in anaesthetized rat. Journal of in Pharmaceutical Sciences. 14:11–4.
Sadraei, H., Asghari, G., Hekmatti, A.A. 2003a. Antispasmodic effect of three fractions of hydroalcoholic extract of Pycnocycla spinosa. Journal of Ethnopharmacology. 86(2-3):187-90. doi: 10.1016/s0378-8741(03)00077-1.
Sadraei, H., Asghari, G., Khazael, M. 2008. Relaxant activities of four frac‌tions separated from alkaloid extract of Pycnocycla spinosa on rat isolated ileum. Research in Pharmaceutical Sciences. 3(2):9-14.
Sadraei, H., Asghari, G., Naddafi, A. 2003b. Relaxant effect of essential oil and hydro-alcoholic extract of Pycnocycla spinosa Decne. Ex Boiss. on ileum contractions. Phytotherapy Research.17(6):645-9. doi: 10.1002/ptr.1217.
Wang, X.J., Luo, Q., Li, T., Meng, P.H., Pu, Y.T., Liu, J.X., Zhang, J., Liu, H., Tan, G.F., Xiong, A.S. 2022. Origin, evolution, breeding, and omics of Apiaceae: a family of vegetables and medicinal plants. Horticulture Research. 9: uhac076. doi: 10.1093/hr/uhac076.
Wen, J., Xie, D.F., Price, M., Ren, T., Deng, Y.Q., Gui, L.J., Guo, X.L. and He, X.J. 2021. Backbone phylogeny and evolution of Apioideae (Apiaceae): New insights from phylogenomic analyses of plastome data. Molecular Phylogenetics and Evolution, 161, p.107183. doi: https://doi.org/10.1016/j.ympev.2021.107183.

  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405