مقایسه خواص ضد سرطانی عصاره های Nepeta binaludensis و Nepeta glomerulosa، بر رده سلولی AGS سرطان معده با تأکید بر تغییرات بیان ژن BAX

نویسندگان

واحد یادگار امام خمینی (ره) شهرری، دانشگاه آزاد اسلامی

چکیده
سرطان معده به عنوان یکی از شایع­ترین انواع سرطان­هاست که منجر به مرگ و میر افراد بسیاری در سر تا سر جهان می­شود. گونه­های Nepeta به دلیل طعم و عطر خاص خود در صنایع غذایی مورد توجه قرار گرفته­اند. با توجه به وجود ترکیبات فنلی در این جنس، هدف از پژوهش حاضر، بررسی اثر سیتوتوکسیک عصاره گونه های Nepeta binaludensis و N. glomerulosa بر روی رده سلولی AGS سرطان معده و نیز بررسی تغییرات بیان ژن BAX بود. هر دو گونه نپتا از کوه­های بینالود خراسان جمع­آوری شد و عصاره هیدروالکلی آنها به روش خیساندن استخراج گردید. مقادیر اسید کافئیک و اسید رزمارینیک با استفاده از دستگاه HPLC اندازه گیری شد. اثر سیتوتوکسیک عصاره­ها با تست MTT و بررسی وقوع آپوپتوز با رنگ آمیزی DAPI مورد ارزیابی قرار گرفت. تغییرات بیان ژنBAX به کمک Real time-PCR، مورد سنجش قرار گرفت. مقدار IC50 پس از 48 ساعت، برای عصاره گونه N. binaludensis 3.70، میلی گرم بر میل لیتر و برای عصاره گونه N.glomerulosa 6.92 میلی گرم بر میلی لیتر بدست آمد. القای آپوپتوز تحت هر دو تیمار مشاهده شد؛ اما افزایش معنی دار بیان ژن BAX نسبت به گروه کنترل تحت تاثیر عصاره N. binaludensis که مقدار بیشتری اسید رزمارینیک داشت، مشاهده گردید. بنابراین، استفاده از عصاره این دو گونه می­تواند در مهار رشد سلول­های سرطانی معده موثر باشد. عصاره N. binaludensis، با افزایش معنی­دار بیان ژن BAX موجب القای آپوپتوز در سلول­های سرطان معده شده است و عملکرد موثرتری نسبت به عصاره N. glomerulosa داشته است.

کلیدواژه‌ها


عنوان مقاله English

Comparison of anticancer properties of Nepeta binaludensis and Nepeta glomerulosa extracts on AGS gastric cancer cell line focusing on changes in BAX gene expression

نویسندگان English

Golaleh Mostafavi
Asal Tofangchi Mahyari
Seyyedeh Mahdokht Maddah
Department of Biology, YI.C., Islamic Azad University, Tehran, Iran
چکیده English

Gastric cancer is one of the most common cancers, causing significant mortality worldwide. Nepeta species, known for their distinct aroma and taste, have garnered attention in the food industry. Given their phenolic compounds, this study aimed to evaluate the cytotoxic effects of Nepeta binaludensis and N. glomerulosa extracts on AGS gastric cancer cell lines and assess changes in BAX gene expression. Both species were collected from the Binalud Mountains in Khorasan, and their hydroalcoholic extracts were prepared via maceration. The amounts of caffeic acid and Rosmarinic acid were measured using HPLC. The extracts' cytotoxic effects were evaluated with the MTT assay, and apoptosis induction was assessed via DAPI staining. Changes in BAX gene expression were measured using real-time PCR. The IC50 values after 48 hours were 3.70 mg/mL for N. binaludensis extract and 6.92 mg/mL for N. glomerulosa extract. Apoptosis induction was observed under both treatments, but N. binaludensis extract, which contained higher Rosmarinic acid, significantly increased BAX expression compared to the control group. Thus, these extracts may inhibit gastric cancer cell growth, with N. binaludensis showing greater efficacy through BAX-mediated apoptosis induction compared to N. glomerulosa.

کلیدواژه‌ها English

Apoptosis
Nepeta binaludensis
Nepeta glomerulosa
cancer cell proliferation
Lamiaceae
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  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405