بررسی تأثیر دیابت نوع دو بر ساختار پیاز بویایی رت نژاد ویستار

نویسندگان

1 گروه زیست شناسی، دانشکده علوم، دانشگاه گلستان، گرگان، ایران

2 گروه فیزیولوژی، مرکز تحقیقات علوم اعصاب، دانشکده پزشکی، دانشگاه علوم پزشکی گلستان، گرگان، ایران

چکیده
حس بویایی نقش مهمی در ایمنی، تغذیه، تعاملات اجتماعی، عملکرد جنسی و کیفیت زندگی فرد دارد. در حال حاضر بیش از 400 میلیون نفر در سراسر جهان به دیابت شیرین مبتلا هستند که یافته ها نشان می­‌دهد این بیماری با اختلال عملکرد بویایی مرتبط است. در پژوهش حاضر ساختار پیاز بویایی رت­های دیابتی نوع 2 مورد ارزیابی قرار گرفت. القاء دیابت با تجویز 20 میلی‌گرم بر کیلوگرم استرپتوزوتوسین به موش های صحرایی بالغ نژاد ویستار به مدت 5 روز انجام شد. پس از 35 روز از گذشت تزریق، به منظور ارزیابی ساختار بویایی، حیوانات بی‌هوش شده و بافت پیاز بویایی برای پردازش بافت و رنگ‌آمیزی نیسل استخراج گردید. بر اساس یافته­ها، تعداد گلومرول‌ها در رت‌های دیابتی نسبت به گروه کنترل کاهش معنی‌داری را نشان داد (05/0P<). علاوه بر این، کاهش قابل توجهی در لایه پلکسی فرم داخلی در پیاز بویایی رت‌های دیابتی در مقایسه با گروه کنترل مشاهده شد (05/0P<). نتایج حاضر نشان داد که اختلال بویایی در رت­های دیابتی نوع 2 با تغییرات ساختاری پیاز بویایی مرتبط است، که نشان دهنده تخریب و آسیب ناشی از دیابت بر بافت پیاز بویایی است.










کلیدواژه‌ها


عنوان مقاله English

The effect of type 2 diabetes on the olfactory bulb structure of Wistar rats

نویسندگان English

Hamidreza Yahyaeipour 1
Farzaneh Ganji 1
Hamid Sepehri 2
Zahra Nazari 1
1 Department of Biology, School of Sciences, Golestan University, Gorgan, Iran
2 Department of Physiology, Neuroscience Research Center, Golestan University of Medical Science, Gorgan, Iran
چکیده English

Olfaction, the sense of smell, plays an important role in the individual's safety, nutrition, social interactions, sexual function, and quality of life. Diabetes mellitus, which affects more than 400 million people worldwide, is associated with olfactory dysfunction. This work evaluated the olfactory bulb structure of Type 2 diabetes rats. Type 2 diabetes induction was performed by administering 20mg/Kg Streptozotocin to adult Wistar rats for five days. 35 days after injection, animals were sacrificed, and olfactory bulb tissue was harvested for tissue processing and Nissl staining to evaluate the olfactory bulb structure. Based on the results, diabetic animals showed a significant decrease in the Glomeruli number compared to controls (P<0.05). Furthermore, we observed a significant decrease in the internal plexiform layer thickness in the olfactory bulb of diabetic rats when compared to controls (P<0.05). The present results showed that olfactory dysfunction in Type 2 diabetes rats is associated with olfactory bulb structural change, which indicates the olfactory bulb tissue damage caused by diabetes.


کلیدواژه‌ها English

Diabetes mellitus
Glomeruli
Nissl staining
Sense of smell
Streptozotocin
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  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405