اثر افزایشی میدان الکترومغناطیسی بر بیان فاکتور رونویسی الیگودندروسیت 1 و 2 (Olig1/2) در کورتکس مغز موش

نویسندگان

گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران

چکیده
Olig1 و Olig2 دو فاکتور کپی برداری است که نقش تنظیم کنندگی در تمایز و اختصاصی شدن سلولهای اجدادی اولیگودندروسیت بازی میکنند. در این مطالعه اثر میدان الکترومغناطیسی بر غلظت کل پروتئین و بیان Olig1 و Olig2 در کورتکس مغز موش بررسی شد. بیست و یک موش نژاد Balb/c به سه گروه کنترل، تیمار و شم (تعداد 7 موش برای هر گروه) تقسیم شدند. موشهای گروه تیمار درون بوبین (سولنوئید) بطور روزانه در معرض میدان 50 هرتز /1 میلی‌تسلا برای 6 ساعت در هر روز و به مدت 10 روز قرار داده شد. گروه شم درون همان بوبین ولی بدون میدان الکترومغناطیس قرار داده شد. بعد از اتمام این مدت موشها بیهوش شده و کورتکس مغز آنها برای آنالیز های بعدی جدا گردید. غلظت کل پروتئین و بیان Olig1 و Olig2 به ترتیب توسط اندازه گیری پروتئین Bio-Rad و وسترن بلات بررسی شد. تفاوت معنی داری در غلظت کل پروتئین در کورتکس مغز گروه تیمار در مقایسه با گروههای شم و کنترل دیده نشد. همچنین نشان داده شد که بیان Olig1 و Olig2 در کورتکس مغز موش گروه تیمار در مقایسه با گروههای شم و کنترل بیشتر است. نتیجه گیری میشود که میدان الکترومغناطیسی باعث افزایش بیان Olig1 و Olig2 در کورتکس مغز موش میشود. به علاوه چون Olig1 و Olig2 نقش مهمی در تکوین سلولهای اجدادی اولیگودندروسیت بازی می کند، نتیجه گیری می شود که EMF ممکن است با افزایش بیان Olig1 و Olig2 در تمایز سلولهای اجدادی اولیگودندروسیت نقش داشته باشد. مطالعه بیشتر برای تایید اثر میدان الکترومغناطیسی بر تمایز اولیگودندروسیت ضروری است.



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کلیدواژه‌ها


عنوان مقاله English

The enhancing effect of electromagnetic field on the expression of Oligodendrocyte transcription factor 1 and 2 (Olig1/2) in the mice cerebral cortex

نویسندگان English

Farhad Mashayekhi
Somaye Shabani
Soheila Talesh Sasani
Zivar Salehi
Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran
چکیده English

Olig1 and Olig2, two transcription factors, play regulatory function in the differentiation and specification of oligodendrocyte progenitor cells (OPCs). In this study the effects of electromagnetic fields (EMF) on total protein concentration ( TPC ) and Olig1 and Olig2 expression in the cerebral cortex of mouse was examined. Twenty-one Balb/c mice were separated into three groups: control, EMF and Sham groups (n=7 for each group). The mice were placed inside the solenoid for a daily EMF exposure of 50 Hz, 1 mT for 6 h/day, 7 days/week for 10 days. The Sham group was also located in the same coil with no exposure. Mice were anesthetized after the final exposure session and their cerebral cortex were collected. TPC and the expression of Olig 1 and Olig2 were studied by Bio-Rad protein assay and western blot, respectively. The cerebral cortex samples were removed for further analysis. There was no significant difference in TPC in the EMF treated cortical samples as compared with those from the SHAM and control groups. It was also shown that the expression of Olig1 and Olig2 was increased in the EMF treated cortical extracts as compared with those in controls and SHAM groups. Therefore, it could be concluded that EMF enhances Olig1 and Olig2 expression in the mice cerebral cortex. Moreover, as Olig1 and Olig2 plays important role in the development of oligodendrocyte progenitor cells, it can be deduced that EMF may affect OPC differentiation by increasing the expression of Olig1 and Olig2. Further studies are needed to clarify the extent of EMF impact on oligodendrocyte differentiation.


کلیدواژه‌ها English

cortex
Gene expression
gliogenesis
magnetic radiation
Western blot
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  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405