بررسی اثر نانوذرات اکسید روی سنتز شده به روش شیمی سبز با دنا

نویسندگان

1 گروه زیست شناسی، دانشکده علوم پایه، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران

2 گروه علوم سلولی و مولکولی، دانشکده علوم زیستی، دانشگاه خوارزمی، تهران، ایران

چکیده
در این مطالعه از نانوذرات اکسید روی سنتزشده با عصاره پودر قهوه که دارای سازگاری با محیط زیست بوده و در انجام فرایند واکنش، هیچگونه مواد سمی و مضری تولید نمی­کند، استفاده شده است. تعامل نانوذرات اکسید روی با DNA تیموس گوساله با استفاده از روش­های مختلف طیف سنجی مرئی فرابنفش، فلورسانس و تکنیک دورنگ نمایی حلقوی مورد بررسی قرار گرفت. داده­های طیف سنجی مرئی فرابنفش نشان داد که با افزایش غلظت نانوذرات اکسید روی، شدت جذب DNA نیز افزایش می­یابد و این افزایش در نانوذرات اکسید روی سنتزسبز در دمای اتاق و فیزیولوژیک انجام شد. نشر فلورسانس اتیدیوم بروماید نشان داد که با افزایش نانوذره اکسید روی شدت نشر کاهش می­یابد که نشان­دهنده ورود نانوذره به ساختار DNA است. همچنین داده­های دورنگ نمایی حلقوی نشان داد که اکسید روی سنتزشده باعث تغییر ساختاری در DNA می­شود. نانوذره اکسید روی سنتزسبز می­تواند به DNA متصل شده و سبب ایجاد تغییر در ساختار DNA شود که می­تواند جایگزین نانوذره اکسید روی شیمیایی شود.

کلیدواژه‌ها


عنوان مقاله English

The investigation of the effects of synthesized Zinc oxide nanoparticles on the DNA using green chemistry

نویسندگان English

Mahnaz Jangi 1
Azadeh Mohammadgholi 1
Adele Divsalr 2
1 Departement of Biology, Faculty of Basic Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2 Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
چکیده English

In this study, the extract of coffee powder for green synthesis of zinc oxide nanoparticles has been used because it is compatibility with the environment and it does not produce any toxic substances in the reaction. Then, the interaction of zinc oxide nanoparticles with calf thymus DNA with various spectroscopic methods such as UV-Visible, fluorescence and circular dichroism (CD) techniques was investigated. UV-Visible data showed that zinc oxide nanoparticles induced denaturation in DNA in a dose-dependent manner at both the room and physiologic temperatures. Extrinsic fluorescence emission of ethidium bromide (EB) results also showed that the increase of zinc oxide nanoparticles concentrations, decreases the emission intensity of EB. This may be the result of the intercalation of nanoparticles. Also, CD data showed that the synthesized zinc oxide caused structural changes in DNA. Finally, according to our results, it can be concluded that zinc oxide nanoparticles can bind with DNA and induce some structural changes in DNA structure.


کلیدواژه‌ها English

coffee powder extract
calf thymus
deoxyribonucleic acid
fluorescence
Uv-visible
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دوره 7، شماره 2 - شماره پیاپی 24
تابستان 1399
صفحه 145-152

  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405