Nova Biologica Reperta

The optimization of Naringenin biosynthesis pathway using Yarrowia lipolitica cell culture

Authors

Monireh Marsafari 1
Habibollah Samizadeh Lahiji 1
Babak Rabiei 1
Ali Ashraf Mehrabi 2
Yongkun Lv 3
Peng Xu 4

1 Faculty of Agricultural Science, University of Guilan, Rasht, Iran

2 Faculty of Agriculture, University of Ilam, Ilam, Iran

3 Faculty of Biotechnology, Jiangnan University, Jiangsu, China

4 Department of Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County, Baltimore, USA

https://doi.org/10.52547/nbr.7.2.133
Abstract
Yarrowia lipolytica, as a good cell factory to speed up the production of plant pharmaceutical components, has been considered to be one of the most important and attractive micro-organisms in recent years, due to its high secretion capacity, limited glycosylation, large range of genetic markers and molecular tools. Naringenin, as a central core of flavonoids production, plays important roles both in plants and in the treatment of different types of human diseases. For this purpose, specific naringenin biosynthesis genes from different origins were selected and introduced after comparative expression profiling in Y. lipolytica. This research indicated that chs plays the main role in the production of naringenin, so the increase copy number of this gene in each construct was investigated. The HPLC results confirmed that the construct with 5 copy numbers of chs resulted in 7.14 fold increase of naringenin extracellular titer to 90.16 mg/L in shake flask cultures. The results reported in this study demonstrated that sufficient knowledge of genes involved in the specific biosynthesis pathway, synthetic gene pathway and using Y. lipolytica as a capable and cheap host could help bioengineers to produce significant amounts of pharmaceutical components.


Keywords

gene copy number
HPLC
metabolic engineering
metabolic flux
yeast host
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Nova Biologica Reperta
Volume 7, Issue 2 - Serial Number 24
Summer 2020
Pages 133-144

  • Receive Date 08 June 2026
  • Publish Date 08 June 2026

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