یافته های نوین در علوم زیستی

بهینه سازی انتقال ژن به گیاه گوجه فرنگی و بررسی امکان بیان ژن مقاومت به شوری (SOS3)

نویسندگان

الهام محجل کاظمی 1
مقصود پژوهنده 2
پریسا جنوبی 3
مینا کاظمیان 1

1 گروه علوم گیاهی دانشکده علوم طبیعی، دانشگاه تبریز، تبریز، ایران

2 گروه بیوتکنولوژی دانشکده کشاورزی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران

3 گروه زیست شناسی گیاهی دانشکده علوم زیستی، دانشگاه خوارزمی، تهران، ایران

https://doi.org/10.29252/nbr.7.1.76
چکیده
یکی از روش‌هایی که از طریق آن می­توان مقاومت به شوری در گیاه را بهبود بخشید بیان بیشینه ژن‌هایی است که بوسیله تنش شوری القا می‌شوند و در برقراری تعادل یونی و اسمزی در گیاه نقش ایفا می‌کنند. ژن SOS3 یکی از مهم‌ترین ژن‌های مسیر SOS است، بنابراین افزایش بیان این ژن می­تواند قدم مهمی در تولید گیاهان مقاوم به شوری باشد. در این پژوهش رقم­های متفاوت گوجه‌فرنگی (Solanum lycopersicum) بواسطه آگروباکتریوم دارای پلاسمید حاوی ژن SOS3 تراریخت شد که بیشترین درصد تراریختی مربوط به برگ لپه‌ای رقم Falat CH بود. استفاده همزمان لپه­ها و هیپوکوتیل در محیط کشت در هنگام آغشتگی بهترین نتیجه را حاصل کرد. علاوه بر این بهترین زمان برای همکشتی، یک روز بعد از تلقیح مشخص گردید و همچنین بهترین سویه آگروباکتریوم جهت تراریختی، سویه GV3101 تشخیص داده شد که می­ توان این امر را به دلیل اثر متقابل نژاد آگروباکتریوم و رقم گوجه ­فرنگی دانست. استفاده از آهن سکسترون در محیط کشت گیاهان تراریخت به طور مشخص باعث سبزی گیاهان و تسریع در رشد آن­ها گردید. در نهایت با توجه به نتایج مولکولی، حضور ژن SOS3 در گیاهان تراریخت به وسیله PCR و RT-PCR تایید شد که نشانگر حضور و بیان ژن مذکور در این گیاهان است.

کلیدواژه‌ها

آگروباکتریوم
باززایی
تراریخت
لپه
هیپوکوتیل

عنوان مقاله English

The optimization of gene transfer to tomato and the study of expression possibility of salt-tolerance gene (SOS3)

نویسندگان English

Elham Mohajel Kazemi 1
Maghsoud Pazhohandeh 2
Parisa Jonoubi 3
Mina Kazemian 1
1 Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Iran
2 Department of Biotechnology, Faculty of Agriculture, Azarbijan Shahid Madani University, Tabriz, Iran
3 Department of Plant Biology, Faculty of Biology Sciences, University of Kharazmi, Iran
چکیده English

One of the main strategies to improve plant tolerance is the expression of stress-induced genes, which play a significant role in the ionic balance of plants. SOS3 is one of the important components of SOS-regulated ionic homeostasis pathway. Therefore, the expression of this gene could be an important step towards producing salt-resistant plants. In this work, we have transformed tomato (Solanum lycopersicum) by Agrobacterium (GV3101 and LBA4404) containing plasmids with SOS3 genes. The maximum regeneration rate was determined in cotyledons of CH genotype. The simultaneous use of cotyledons and hypocotyls in the culture medium had the best outcome. In addition, the best time was found to be one day after inoculation. Also, the best transgenic variety was detected for Agrobacterium GV3101, which can be attributed to the interaction between the genus Agrobacterium and the tomato variety. Transgenic plants were transferred to a culture medium containing sequestrene, which caused the acceleration of the seedling growth in particular. The presence of the SOS3 in the transgenic plants was verified by PCR and RT-PCR methods.






کلیدواژه‌ها English

agrobacterium
cotyledon
hypocotyl
Regeneration
transgene
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