یافته های نوین در علوم زیستی

اثر ترمیم کننده پلی ساکارید استخراج شده از ستاره شکننده بر زخم پوستی موش صحرایی نر نژاد ویستار

نویسندگان

ایلناز ابوالحسنی 1
جواد بهارآرا 1
ناصر مهدوی شهری 2
الهه امینی 3

1 گروه زیست شناسی، دانشکده علوم، دانشگاه آزاد اسلامی، مشهد، ایران

2 گروه زیست شناسی، دانشکده علوم، دانشگاه فردوسی، مشهد، ایران

3 گروه علوم جانوری، دانشکده علوم زیستی، دانشگاه خوارزمی، تهران، ایران

https://doi.org/10.29252/nbr.7.1.9
چکیده
در طب سنتی عصاره­ های گیاهان دربردارنده پلی ­ساکارید به ­طور وسیعی برای درمان زخم پوستی به­ کار می­ رود. ستاره­ شکننده به عنوان یک موجود دریایی دارای ترکیبات فعال زیستی است که به آن قدرت بازسازی بازوهای آسیب دیده را می­ دهد. هدف مطالعه حاضر بررسی اثر پلی­ ساکارید استخراج شده از ستاره ­شکننده خلیج فارس (Ophiocoma erinaceus) در ترمیم زخم پوستی موش صحرایی نر نژاد ویستار است. در این پژوهش تجربی، 60 سر موش صحرایی نر به­ طور تصادفی به 5 گروه شاهد، شاهدمثبت (تیمار با عسل)، گروه تجربی1 تا 3 به ترتیب از راست به چپ تحت تیمار با دوز 12/5، 25 و 37/5 میلی­ گرم/کیلوگرم وزن بدن پلی­ ساکارید ستاره ­شکننده تقسیم شدند. زخم در بخش پشتی بدن ایجاد شد و موش­ ها تحت تیمار موضعی قرار گرفتند. در روزهای 3، 7، 10 و 14 از زخم­ های در حال ترمیم نمونه­ برداری شد و پس از رنگ­ آمیزی توسط میکروسکوپ نوری ارزیابی شد. سپس، داده ­های کمی توسط نرم افزار SPSS ، آزمون آماری آنالیز واریانس یک طرفه تحلیل شدند. نتایج میکروسکوپی نشان داد که در زخم­ های تیمار شده با دوز 37/5 پلی­ ساکارید مشابه با گروه شاهد مثبت بازسازی اپی­تلیوم، رگ­زایی و کاهش تعداد سلول­ های التهابی سریع­ تر ازگروه شاهد بود. بیش­ترین ضخامت اپی­تلیوم در روز 7 و کم­ترین تعداد سلول­های التهابی در روز 14 در گروه تجربی 3 و شاهد مثبت نسبت به گروه شاهد مشاهده شد. یافته­ های این پژوهش نشان داد که پلی ­ساکارید ستاره­ شکننده باعث تسریع روند ترمیم زخم می­ شود که می­ تواند به عنوان یک ترکیب طبیعی در تکوین داروهای ترمیم ­دهنده زخم پوستی پیشنهاد شود.

کلیدواژه‌ها

التیام زخم
ترکیبات فعال زیستی
خلیج فارس
کربوهیدرات
مدل آزمایشگاهی

عنوان مقاله English

The regenerative properties of the extracted polysaccharide from Brittle star (Ophiocoma erinaceus) on cutaneous wound in male Wistar rat

نویسندگان English

Ilnaz Abolhasani 1
Javad Baharara 1
Naser Mahdavi Shahri 2
Elaheh Amini 3
1 Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran
2 Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
3 Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Iran
چکیده English

In traditional medicine, extracts of polysaccharide-containing plants are widely employed for the treatment of cutaneus wounds. The brittle star, as one of marine organisms, encompasses bioactive compounds, which confer the healing potency of damaged arms. The aim of the present study is to investigate the effects of extracted polysaccharide from the Persian Gulf brittle star )Ophiocoma erinaceus( on male Wistar rat skin wound healing. In this experimental study, 60 male Wistar rats were divided randomly into 5 groups including control, positive control (treatment with honey), experimental 1 to 3 (treatment with 12.5, 25, 37.5 mg/kg body weight of extracted polysaccharide, respectively). In all groups, the wound was inflicted in the posterior part of the body of rats. Then, rats were treated locally. On the 3th, 7th, 10th and 14th days, samples were collected from the healing hole and histological changes were investigated by light microscopy. Then, quantitative data were analyzed by SPSS software, one-way ANOVA at the level of p<0.05. Microscopic results showed that, in treated wounds by 37.5 mg/kg of extracted polysaccharide, as well as positive control, the re-epithelialization, the angiogenesis and the attenuation of inflammatory cells were significantly improved in comparison with the control group. Maximum epithelium thickness (on the 7th day) and minimum inflammatory cells (on the 14th day) were observed in the experimental group 3 (treated with 37.5 mg/kg of extracted polysaccharide) and positive control, as compared with the control group (p≤0.05). The findings of this research indicated that the polysaccharide extracted from O. erinaceus accelerated the cutaneus wound healing in male rats, which can be used as a natural component in the development of natural source agents for the treatment of cutaneous wounds.

کلیدواژه‌ها English

animal model
bioactive compounds
carbohydrate
Persian Gulf
wound healing
Alahtavakoli, M., Vazirinejad, R., Ansari, J., Negahban, T., Mashayekhi, H., Nazari, M., Ghoreshi, S. & Nematollahi, F. 2010. Effect of Teucrium polium extract on skin wound healing in rat. Hormozgan Med. J. 16: 17-24.
Alahtavakoli, M., Vazirinejad, R., Ansari, J., Negahban, T., Mashayekhi, H., Nazari, M., Ghoreshi, S. & Nematollahi, F. 2010. Effect of Teucrium polium extract on skin wound healing in rat. Hormozgan Med. J. 16: 17-24.
Agrawal, P., Soni, S., Mittal, G. & Bhatnagar, A. 2014. Role of polymeric biomaterials as wound healing agents. Int. J. Low Extrem. Wounds 13: 180-90. [DOI:10.1177/1534734614544523]
Agrawal, P., Soni, S., Mittal, G. & Bhatnagar, A. 2014. Role of polymeric biomaterials as wound healing agents. Int. J. Low Extrem. Wounds 13: 180-90. [DOI:10.1177/1534734614544523]
Baharara, J. & Amini, E. 2016. The potential of Brittle Star extracted polysaccharide in promoting apoptosis via intrinsic signaling pathway. Avecinna J. Med. Biotechnol. 7: 151-158.
Baharara, J. & Amini, E. 2016. The potential of Brittle Star extracted polysaccharide in promoting apoptosis via intrinsic signaling pathway. Avecinna J. Med. Biotechnol. 7: 151-158.
Baharara, J., Mahdavi Shahri, N. & Shaddel, N. 2014. Local effect of the Persian Gulf Brittle Star (Ophiocoma erinaceus) alcoholic extract on cutaneous wound healing in Balb/C mouse. J. Birjand Uni. Med. Sci. 21: 312-23.
Baharara, J., Mahdavi Shahri, N. & Shaddel, N. 2014. Local effect of the Persian Gulf Brittle Star (Ophiocoma erinaceus) alcoholic extract on cutaneous wound healing in Balb/C mouse. J. Birjand Uni. Med. Sci. 21: 312-23.
Bielefeld, K.A, Amini-Nik, S. & Alman, B.A. 2013. Cutaneous wound healing: recruiting developmental pathways for regeneration. Cell Mol. Life Sci. 70: 2059-2081. [DOI:10.1007/s00018-012-1152-9]
Bielefeld, K.A, Amini-Nik, S. & Alman, B.A. 2013. Cutaneous wound healing: recruiting developmental pathways for regeneration. Cell Mol. Life Sci. 70: 2059-2081. [DOI:10.1007/s00018-012-1152-9]
Cardose, C.R., Souza, M.A., Ferro, E.A., Favoreto, J.R., Pena, J.D. 2004. Influence of topical administration of n-3 and n-6 essential and n-9 nonessential fatty acid on the healing of cataneuos wounds. Wound Repair Regen. 12: 235- 243. [DOI:10.1111/j.1067-1927.2004.012216.x]
Cardose, C.R., Souza, M.A., Ferro, E.A., Favoreto, J.R., Pena, J.D. 2004. Influence of topical administration of n-3 and n-6 essential and n-9 nonessential fatty acid on the healing of cataneuos wounds. Wound Repair Regen. 12: 235- 243. [DOI:10.1111/j.1067-1927.2004.012216.x]
Cragg, G.M. & Newman, D.J. 2013. Natural products : A continuing source of novel drug leads. Biochim. Biophys. Acta. 830: 3670-3695. [DOI:10.1016/j.bbagen.2013.02.008]
Cragg, G.M. & Newman, D.J. 2013. Natural products : A continuing source of novel drug leads. Biochim. Biophys. Acta. 830: 3670-3695. [DOI:10.1016/j.bbagen.2013.02.008]
Czarkwiani, A., Dylus, D.V. & Oliveri, P. 2013. Expression of skeletogenic genes during arm regeneration in the brittle star Amphiura filiformis. Gene Expr. Patterns 13: 464-72. [DOI:10.1016/j.gep.2013.09.002]
Czarkwiani, A., Dylus, D.V. & Oliveri, P. 2013. Expression of skeletogenic genes during arm regeneration in the brittle star Amphiura filiformis. Gene Expr. Patterns 13: 464-72. [DOI:10.1016/j.gep.2013.09.002]
De Zoysa, M. 2012. Medicinal benefits of marine invertebrates: sources for discovering natural drug candidates. Adv. Food Nutr. Res. 153-169. [DOI:10.1016/B978-0-12-416003-3.00009-3]
De Zoysa, M. 2012. Medicinal benefits of marine invertebrates: sources for discovering natural drug candidates. Adv. Food Nutr. Res. 153-169. [DOI:10.1016/B978-0-12-416003-3.00009-3]
Ghasemifard, S. 2011. Wound healing properties of Eucheuma cottonii extracts in Sprague-Dawley rats. J. Med. Plant. Res. 5: 6373-6380. [DOI:10.5897/JMPR10.902]
Ghasemifard, S. 2011. Wound healing properties of Eucheuma cottonii extracts in Sprague-Dawley rats. J. Med. Plant. Res. 5: 6373-6380. [DOI:10.5897/JMPR10.902]
Hameedaldeen, A., Liu, J., Batres, A., Graves, G.S. & Graves, D.T. 2014. FOXO1, TGF-β regulation and wound healing. Int. J. Mol Sci. 15: 16257-69. [DOI:10.3390/ijms150916257]
Hameedaldeen, A., Liu, J., Batres, A., Graves, G.S. & Graves, D.T. 2014. FOXO1, TGF-β regulation and wound healing. Int. J. Mol Sci. 15: 16257-69. [DOI:10.3390/ijms150916257]
Inagaki, M., Shibai, M., Isobe, R. & Higuchi, R. 2001. Constituents of ophiuroidea.1. isolation and structure of three ganglioside molecular species from the brittle star Ophiocoma scolopendrina. Chem. Pharm. Bull. 49: 1521-25. [DOI:10.1248/cpb.49.1521]
Inagaki, M., Shibai, M., Isobe, R. & Higuchi, R. 2001. Constituents of ophiuroidea.1. isolation and structure of three ganglioside molecular species from the brittle star Ophiocoma scolopendrina. Chem. Pharm. Bull. 49: 1521-25. [DOI:10.1248/cpb.49.1521]
Kaboli, H. & Haghighat, S. 2017. Evaluation of borrago topical effects on wound healing of cutting wounds in mice. J. Shahid Sadoughi Univ. Med. Sci. 25: 311-321.
Kaboli, H. & Haghighat, S. 2017. Evaluation of borrago topical effects on wound healing of cutting wounds in mice. J. Shahid Sadoughi Univ. Med. Sci. 25: 311-321.
Lee, K.S., Shin, J.S. & Nam, K.S. 2011. Cancer chemo preventive effects of starfish polysaccharide in human breast cancer cells. Biotech. Bioprocess Eng. 16: 987-991. [DOI:10.1007/s12257-011-0226-0]
Lee, K.S., Shin, J.S. & Nam, K.S. 2011. Cancer chemo preventive effects of starfish polysaccharide in human breast cancer cells. Biotech. Bioprocess Eng. 16: 987-991. [DOI:10.1007/s12257-011-0226-0]
Li, C., Blencke, H.M., Haug, T. & Stensvåg, K. 2015. Anti-microbial peptides in Echinoderm host defense. Dev. Comp. Immunol. 49:190-197. [DOI:10.1016/j.dci.2014.11.002]
Li, C., Blencke, H.M., Haug, T. & Stensvåg, K. 2015. Anti-microbial peptides in Echinoderm host defense. Dev. Comp. Immunol. 49:190-197. [DOI:10.1016/j.dci.2014.11.002]
Miguel, D., António, G., Maria, A. & Nobre, M. 2012. Characterization of the coelomic fluid of the starfish Marthasterias glacialis in a wound-healing phase. IST 2: 1-73.
Miguel, D., António, G., Maria, A. & Nobre, M. 2012. Characterization of the coelomic fluid of the starfish Marthasterias glacialis in a wound-healing phase. IST 2: 1-73.
Mirjalili, S.M., Faramarzi, S., Esmaeilideha,j M., Zare Mehrjardi, F. & Rezvani, M.E. 2017. Effect of aqueous extract of Morus nigra on skin wound healing in type 1 diabetic rats. J. Shahid Sadoughi Univ. Med. Sci. 25: 264-70.
Mirjalili, S.M., Faramarzi, S., Esmaeilideha,j M., Zare Mehrjardi, F. & Rezvani, M.E. 2017. Effect of aqueous extract of Morus nigra on skin wound healing in type 1 diabetic rats. J. Shahid Sadoughi Univ. Med. Sci. 25: 264-70.
Prabhu, K. & Bragadeeswaran, S. 2013. Biological properties of brittle star Ophiocnemis marmorata collected from parangipettai, southeast coast of India. J. Microbiol. Antimicrob. 5: 110-118. [DOI:10.5897/JMA2013.0270]
Prabhu, K. & Bragadeeswaran, S. 2013. Biological properties of brittle star Ophiocnemis marmorata collected from parangipettai, southeast coast of India. J. Microbiol. Antimicrob. 5: 110-118. [DOI:10.5897/JMA2013.0270]
Salama, M., Elgadir, M.A. & Adam, A. 2014. Antioxidant, Antimicrobial and Antiinflammatory effects of selected plants on wound healing. World J. Pharmacy. Pharmaceutical. Sci. 3: 1-11.
Salama, M., Elgadir, M.A. & Adam, A. 2014. Antioxidant, Antimicrobial and Antiinflammatory effects of selected plants on wound healing. World J. Pharmacy. Pharmaceutical. Sci. 3: 1-11.
Silva, T.H., Alves, A., Ferreira, B.M., Oliveira, J.M., Reys, L.L. & Ferreira, R.J.F. 2012. Materials of marine origin: a review on polymers and ceramics of biomedical interest. Int. Materials Rev. 57: 276-306. [DOI:10.1179/1743280412Y.0000000002]
Silva, T.H., Alves, A., Ferreira, B.M., Oliveira, J.M., Reys, L.L. & Ferreira, R.J.F. 2012. Materials of marine origin: a review on polymers and ceramics of biomedical interest. Int. Materials Rev. 57: 276-306. [DOI:10.1179/1743280412Y.0000000002]
Smith, L.C., Ghosh, J., Buckley, K.M., Clow, L.A., Dheilly, N.M. & Haug, T. 2010. Echinoderm immunity. Adv. Exp. Med. Biol. 708: 260-271. [DOI:10.1007/978-1-4419-8059-5_14]
Smith, L.C., Ghosh, J., Buckley, K.M., Clow, L.A., Dheilly, N.M. & Haug, T. 2010. Echinoderm immunity. Adv. Exp. Med. Biol. 708: 260-271. [DOI:10.1007/978-1-4419-8059-5_14]
Soheili, A., Baharara, J., Mahdavi Shahri, N., Zafar Balanejad, S. & Amini, E. 2016. Protective Effect of the Persian Gulf brittle star Ophiocoma erinaceus extract on carbon tetrachloride (CCl4) induced liver damage in adult male Wistar rat. J. Birjand Uni. Med. Sci. 22: 316-326.
Soheili, A., Baharara, J., Mahdavi Shahri, N., Zafar Balanejad, S. & Amini, E. 2016. Protective Effect of the Persian Gulf brittle star Ophiocoma erinaceus extract on carbon tetrachloride (CCl4) induced liver damage in adult male Wistar rat. J. Birjand Uni. Med. Sci. 22: 316-326.
Sohrabifar, N., Baharara, J., Mahdavi Shahri, N., Zafar Balanejad, S. & Amini, E. 2016. The impact of sheep testis extract on hair follicle growth and skin healing of Wistar rats. Nova Biol. Reperta 3: 24-38. [DOI:10.21859/acadpub.nbr.3.1.24]
Sohrabifar, N., Baharara, J., Mahdavi Shahri, N., Zafar Balanejad, S. & Amini, E. 2016. The impact of sheep testis extract on hair follicle growth and skin healing of Wistar rats. Nova Biol. Reperta 3: 24-38. [DOI:10.21859/acadpub.nbr.3.1.24]
Suntar, I., Tatli, I., Kupeli Akkol, E. & Keles, H. 2010. An ethnopharmaclogical study on Verbascum species: from conventional wound healing use to scientific verification. J. Ethnopharmacol. 132: 8-13. [DOI:10.1016/j.jep.2010.08.004]
Suntar, I., Tatli, I., Kupeli Akkol, E. & Keles, H. 2010. An ethnopharmaclogical study on Verbascum species: from conventional wound healing use to scientific verification. J. Ethnopharmacol. 132: 8-13. [DOI:10.1016/j.jep.2010.08.004]
Zueva, O., Khoury, M., Heinzeller, T., Mashanova, D. & Mashano, V. 2018. The complex simplicity of the brittle star nervous system. Front. Zool. 15:1-26. [DOI:10.1186/s12983-017-0247-4]
Zueva, O., Khoury, M., Heinzeller, T., Mashanova, D. & Mashano, V. 2018. The complex simplicity of the brittle star nervous system. Front. Zool. 15:1-26. [DOI:10.1186/s12983-017-0247-4]

  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405

صفحه اصلی

راهنمای نویسندگان

ارسال مقاله

داوران

تماس با ما