یافته های نوین در علوم زیستی

بررسی ارتباط ماهیان کور غار لرستان (Garra thyphlops و Garra lorestanensis) و جمعیت های ماهی گلچراغ (Garra gymnothorax) در حوضه های دز و کرخه

نویسندگان

ایرج هاشم زاده 1
سیده نرجس طباطبائی 2
نبی الله قائدرحمتی 3
محسن امیری 3
لوئیس برناتچز 4

1 1 گروه شیلات و محیط زیست دانشکده منابع طبیعی و علوم زمین دانشگاه شهرکرد، شهرکرد، ایران

2 گروه تنوع زیستی و مدیریت اکوسیستم، پژوهشکده علوم محیطی، دانشگاه شهید بهشتی، تهران، ایران

3 اداره کل حفاظت محیط زیست استان لرستان، لرستان، ایران

4 گروه زیست شناسی، دانشگاه لاوال، کبک، کانادا

https://doi.org/10.29252/nbr.7.1.1
چکیده
غار محل زیست ماهیان کور لرستان در حوضه رود دز در مجاورت رودخانه سزار در منطقه ­ای با ساختار کارستی قرار گرفته است. در مجاورت رودخانه سزار در دیواره­ های دره رودخانه چشمه­ هایی وجود دارد که ممکن است امکان ورود ماهیان رودخانه ­ای را به داخل منابع آب زیرزمینی فراهم کنند. این مشاهدات احتمال رابطه مهاجرتی در بین ماهیان gymnothorax Garra رودخانه سزار و ماهیان کور typhlops Garra و Garra lorestanensis را پیش می ­آورد. علاوه بر این در بین ماهیان کور در مورد هر گونه، تنوع شکل بدن قابل مشاهده است. تنوع فنوتیپی یادشده ممکن است گویای تنوع ژنتیکی ناشناخته ­ای در بین این ماهیان باشد و یا ناشی از شرایط محیطی حاکم بر بخش­ های مختلف غار ماهی کور باشد. در این مطالعه برای پاسخ به سوالات موجود از روش­ های تعیین توالی ژن سیتوکروم اکسیداز زیرواحد یک (COI) و تعیین توالی نسل جدید استفاده شده است. نتایج نشان می­ دهد اشکال دوکی شکل و اشکال دارای بدن کشیده و غیر دوکی گونه ­های typhlops G. و lorestanensis G. از نظر ترکیب ژنوم و ژن میتوکندریایی تفاوتی ندارند. همچنین نتایج مقایسه ترکیب ژنومی ماهیان کور و ماهی gymnothorax G. رودخانه­ های دز و کرخه نشان می­ دهد احتمالا نفوذ ژنتیکی بسیار کمی (کمتر از 3 درصد) از گونه gymnothorax G. به ماهی کور typhlops G. وجود دارد. شاید بتوان این نفوذ ژنتیکی کم ماهیان سطح­ زی را به سازگاری پایین­ تر آن­ها برای ورود و انطباق با شرایط زیستگاه زیرزمینی نسبت داد.

کلیدواژه‌ها

ترکیب ژنوم
تنوع شکل
توالی یابی نسل جدید
ژنوم میتوکندریایی
غار ماهی کور

عنوان مقاله English

The analysis of the relationship between Lorestan cave barbs (Garra typhlops and Garra lorestanensis) and Garra gymnothorax populations in Dez and Karkheh River drainages

نویسندگان English

Iraj Hashemzadeh 1
Seyedeh Narjes Tabatabaei 2
Nabiallah Ghaed Rahmati 3
Mohsen Amiri 3
Louis Bernatchez 4
1 Department of Fisheries and Environmental Sciences, Faculty of Natural Resources and Earth Sciences, Shahr-e-Kord University, Shahr-e-Kord, Iranد
2 Department of Biodiversity and Ecosystem Management, Environmental sciences Research Institute, Shahid Beheshti University, Tehran, Iran
3 Lorestan Province Department of Environment, Khoramabad, Iran
4 Department of Biology, Laval University, Quebec, Canada
چکیده English

The cave barb habitat is located in a Karst formation along the Sezar River. The springs on the walls of the Sezar River valley may provide a means for fish in surface waters to penetrate into the underground waters. These observations propose the probability for a migratory relationship between Garra gymnothorax in the Sezar River and the cave barbs (Garra typhlops and Garra lorestanensis). In addition, a variety of different body shapes including fusiform and slender body forms are observed among the cave fish. This phenotypical variation may be a sign of an unknown genetic diversity or could be attributed to the variable environmental conditions in different parts of the subterranean habitat. To clarify the situation, we used the sequences of mtDNA cytochrome oxidase subunit I and next generation sequencing method. The results showed that the fusiform and slender body shapes of G. typhlops and G. lorestanensis were not different with regard to their mtDNA and genomic compositions. Moreover, the analysis of the genomic their mtDNA and genomic compositions. Moreover, the analysis of the genomic showed that a limited level of gene flow (less than 3%) from G. gymnothorax probably existed in G. thyphlops. The low level of gene flow may be related to the lower fitness and adaptability of the surface dwelling fish to the subterranean life conditions.


کلیدواژه‌ها English

Cave barb locality
genome composition
mitochondrial genome
morphological diversity
next generation sequencing
Alexander, D.H. & Lange, K. 2011. Enhancements to the ADMIXTURE algorithm for individual ancestry estimation. BMC Bioinform. 12: 246. [DOI:10.1186/1471-2105-12-246]
Alexander, D.H. & Lange, K. 2011. Enhancements to the ADMIXTURE algorithm for individual ancestry estimation. BMC Bioinform. 12: 246. [DOI:10.1186/1471-2105-12-246]
Aljanabi, S.M. & Martinez, I. 1997. Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Res. 25: 4692-4693. [DOI:10.1093/nar/25.22.4692]
Aljanabi, S.M. & Martinez, I. 1997. Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Res. 25: 4692-4693. [DOI:10.1093/nar/25.22.4692]
Benestan, L., Gosselin, T., Perrier, C., Sainte‐Marie, B., Rochette, R. & Bernatchez, L. 2015. RAD genotyping reveals fine‐scale genetic structuring and provides powerful population assignment in a widely distributed marine species, the American lobster (Homarus americanus). Mol. Ecol. 24: 3299-3315. [DOI:10.1111/mec.13245]
Benestan, L., Gosselin, T., Perrier, C., Sainte‐Marie, B., Rochette, R. & Bernatchez, L. 2015. RAD genotyping reveals fine‐scale genetic structuring and provides powerful population assignment in a widely distributed marine species, the American lobster (Homarus americanus). Mol. Ecol. 24: 3299-3315. [DOI:10.1111/mec.13245]
Catchen, J., Hohenlohe, P.A., Bassham, S., Amores, A. & Cresko, W.A. 2013. Stacks: an analysis tool set for population genomics. Mol. Ecol. 22: 3124-3140. [DOI:10.1111/mec.12354]
Catchen, J., Hohenlohe, P.A., Bassham, S., Amores, A. & Cresko, W.A. 2013. Stacks: an analysis tool set for population genomics. Mol. Ecol. 22: 3124-3140. [DOI:10.1111/mec.12354]
Coad, B. 2019. Freshwater Fishes of Iran. Available at: www.briancoad.com. Accessed on 14-12-2019.
Coad, B. 2019. Freshwater Fishes of Iran. Available at: www.briancoad.com. Accessed on 14-12-2019.
Decker, J.E., McKay, S.D., Rolf, M.M., Kim, J., Alcalá, A.M., Sonstegard, T.S., Hanotte, O., Götherström, A., Seabury, C.M., Praharani, L. & Babar, M.E. 2014. Worldwide patterns of ancestry, divergence, and admixture in domesticated cattle. PLOS Genet. 10: p.e1004254. [DOI:10.1371/journal.pgen.1004254]
Decker, J.E., McKay, S.D., Rolf, M.M., Kim, J., Alcalá, A.M., Sonstegard, T.S., Hanotte, O., Götherström, A., Seabury, C.M., Praharani, L. & Babar, M.E. 2014. Worldwide patterns of ancestry, divergence, and admixture in domesticated cattle. PLOS Genet. 10: p.e1004254. [DOI:10.1371/journal.pgen.1004254]
Dunham, R.A. 2011. Aquaculture and fisheries biotechnology: genetic approaches. Cabi. Oxfordshire, UK, 494 pp. [DOI:10.1079/9781845936518.0000]
Dunham, R.A. 2011. Aquaculture and fisheries biotechnology: genetic approaches. Cabi. Oxfordshire, UK, 494 pp. [DOI:10.1079/9781845936518.0000]
Farashi, A., Kaboli, M., Rezaei, H.R., Naghavi, M.R., Rahimian, H. & Coad, B.W. 2014. Reassessment of the taxonomic position of Iranocypris typhlops Bruun & Kaiser, 1944 (Actinopterygii, Cyprinidae). ZooKeys 374: 69-77. [DOI:10.3897/zookeys.374.6617]
Farashi, A., Kaboli, M., Rezaei, H.R., Naghavi, M.R., Rahimian, H. & Coad, B.W. 2014. Reassessment of the taxonomic position of Iranocypris typhlops Bruun & Kaiser, 1944 (Actinopterygii, Cyprinidae). ZooKeys 374: 69-77. [DOI:10.3897/zookeys.374.6617]
Hallerman, E.M. 2003. Population genetics: principles and practices for fisheries scientists. American Fisheries Society, Bethesda, MD, 458 pp.
Hallerman, E.M. 2003. Population genetics: principles and practices for fisheries scientists. American Fisheries Society, Bethesda, MD, 458 pp.
Hashemzadeh Segherloo, I., Abdoli, A., Eagderi, S., Esmaeili, H.R., Sayyadzadeh, G., Bernatchez, L., Hallerman, E., Geiger, M.F., Özulug, M., Laroche, J. & Freyhof, J. 2017. Dressing down: convergent reduction of the mental disc in Garra (Teleostei: Cyprinidae) in the Middle East. Hydrobiol. 785: 47-59. [DOI:10.1007/s10750-016-2902-8]
Hashemzadeh Segherloo, I., Abdoli, A., Eagderi, S., Esmaeili, H.R., Sayyadzadeh, G., Bernatchez, L., Hallerman, E., Geiger, M.F., Özulug, M., Laroche, J. & Freyhof, J. 2017. Dressing down: convergent reduction of the mental disc in Garra (Teleostei: Cyprinidae) in the Middle East. Hydrobiol. 785: 47-59. [DOI:10.1007/s10750-016-2902-8]
Hashemzadeh Segherloo, I., Bernatchez, L., Golzarianpour, K., Abdoli, A., Primmer, C.R. & Bakhtiary, M. 2012a. Genetic differentiation between two sympatric morphs of the blind Iran cave barb Iranocypris typhlops. J. Fish Biol. 81: 1747-1753. [DOI:10.1111/j.1095-8649.2012.03389.x]
Hashemzadeh Segherloo, I., Bernatchez, L., Golzarianpour, K., Abdoli, A., Primmer, C.R. & Bakhtiary, M. 2012a. Genetic differentiation between two sympatric morphs of the blind Iran cave barb Iranocypris typhlops. J. Fish Biol. 81: 1747-1753. [DOI:10.1111/j.1095-8649.2012.03389.x]
Hashemzadeh Segherloo, I., Farahmand, H., Abdoli, A., Bernatchez, L., Primmer, C.R., Swatdipong, A., Karami, M. & Khalili, B. 2012b. Phylogenetic status of brown trout Salmo trutta populations in five rivers from the southern Caspian Sea and two inland lake basins, Iran: a morphogenetic approach. J. Fish Biol. 81: 1479-1500. [DOI:10.1111/j.1095-8649.2012.03428.x]
Hashemzadeh Segherloo, I., Farahmand, H., Abdoli, A., Bernatchez, L., Primmer, C.R., Swatdipong, A., Karami, M. & Khalili, B. 2012b. Phylogenetic status of brown trout Salmo trutta populations in five rivers from the southern Caspian Sea and two inland lake basins, Iran: a morphogenetic approach. J. Fish Biol. 81: 1479-1500. [DOI:10.1111/j.1095-8649.2012.03428.x]
Hashemzadeh Segherloo, I., Ghaedrahmati, N. & Freyhof, J. 2016. Eidinemacheilus, a new generic name for Noemacheilus smithi Greenwood (Teleostei; Nemacheilidae). Zootaxa 4147: 466-476. [DOI:10.11646/zootaxa.4147.4.7]
Hashemzadeh Segherloo, I., Ghaedrahmati, N. & Freyhof, J. 2016. Eidinemacheilus, a new generic name for Noemacheilus smithi Greenwood (Teleostei; Nemacheilidae). Zootaxa 4147: 466-476. [DOI:10.11646/zootaxa.4147.4.7]
Hashemzadeh Segherloo, I., Normandeau, E., Benestan, L., Rougeux, C., Coté, G., Moore, J.S., Ghaedrahmati, N., Abdoli, A. & Bernatchez, L. 2018. Genetic and morphological support for possible sympatric origin of fish from subterranean habitats. Sci. Rep. 8: 2909. [DOI:10.1038/s41598-018-20666-w]
Hashemzadeh Segherloo, I., Normandeau, E., Benestan, L., Rougeux, C., Coté, G., Moore, J.S., Ghaedrahmati, N., Abdoli, A. & Bernatchez, L. 2018. Genetic and morphological support for possible sympatric origin of fish from subterranean habitats. Sci. Rep. 8: 2909. [DOI:10.1038/s41598-018-20666-w]
Hubert, N., Hanner, R., Holm, E., Mandrak, N.E., Taylor, E., Burridge, M., Watkinson, D., Dumont, P., Curry, A., Bentzen, P. & Zhang, J. 2008. Identifying Canadian freshwater fishes through DNA barcodes. PLoS One 3: p.e2490. [DOI:10.1371/journal.pone.0002490]
Hubert, N., Hanner, R., Holm, E., Mandrak, N.E., Taylor, E., Burridge, M., Watkinson, D., Dumont, P., Curry, A., Bentzen, P. & Zhang, J. 2008. Identifying Canadian freshwater fishes through DNA barcodes. PLoS One 3: p.e2490. [DOI:10.1371/journal.pone.0002490]
Mousavi-Sabet, H. & Eagderi, S. 2016. Garra lorestanensis, a new cave fish from the Tigris River drainage with remarks on the subterranean fishes in Iran (Teleostei: Cyprinidae). Fish Taxa 1: 45-54.
Mousavi-Sabet, H. & Eagderi, S. 2016. Garra lorestanensis, a new cave fish from the Tigris River drainage with remarks on the subterranean fishes in Iran (Teleostei: Cyprinidae). Fish Taxa 1: 45-54.
Proudlove, G.S. 2006. Subterranean fishes of the world: an account of the subterranean (hypogean) fishes described up to 2003 with a bibliography 1541-2004. International Society for Subterranean Biology, 300 pp.
Proudlove, G.S. 2006. Subterranean fishes of the world: an account of the subterranean (hypogean) fishes described up to 2003 with a bibliography 1541-2004. International Society for Subterranean Biology, 300 pp.
Sargeran, P., Bakhtiyari, M., Abdoli, A., Coad, B.W., Sarvi, K., Lishi, M.R. & Hajimoradloo, A. 2008. The endemic Iranian Cave-fish, Iranocypris typhlops: two taxa or two forms based on the mental disc? Zool. Middle East 44: 67-74. [DOI:10.1080/09397140.2008.10638290]
Sargeran, P., Bakhtiyari, M., Abdoli, A., Coad, B.W., Sarvi, K., Lishi, M.R. & Hajimoradloo, A. 2008. The endemic Iranian Cave-fish, Iranocypris typhlops: two taxa or two forms based on the mental disc? Zool. Middle East 44: 67-74. [DOI:10.1080/09397140.2008.10638290]
Wilkens, H. & Hüppop, K. 1986. Sympatric speciation in cave fishes? J. Zool. Syst. Evol. Res. 24: 223-230. [DOI:10.1111/j.1439-0469.1986.tb00630.x]
Wilkens, H. & Hüppop, K. 1986. Sympatric speciation in cave fishes? J. Zool. Syst. Evol. Res. 24: 223-230. [DOI:10.1111/j.1439-0469.1986.tb00630.x]

  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405

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