یافته های نوین در علوم زیستی

تاثیر ترکیبی بیوپلاستیک و باکتری­ های تجزیه کننده آن از سرده Acidovorax بر فعالیت متابولیکی باکتری­های بی­ هوازی انتهای روده بچه ماهیان تاسماهی سیبری (Acipenser baerii) به روش CLPP

نویسندگان

ابراهیم حسین نجدگرامی 1
پیتر بوسیر 2

1 دانشگاه ارومیه

2 دانشگاه گنت

https://doi.org/10.29252/nbr.6.2.169
چکیده
روش Community Level Physiological Profiles (CLPP) از جمله روش­های نوین در ارزیابی تنوع و فعالیت متابولیکی باکتری­ها در اکوسیستم­ها است. بر اساس نتایج تحقیقات انجام شده، پلی بتا هیدروکسی بوتیرات (PHB) به عنوان یک عامل بیوکنترل، باعث افزایش تنوع باکتریایی در سیستم گوارش برخی از گونه­ های آبزی شده است. در این تحقیق تاثیر استفاده از PHB و ترکیب دو گونه از باکتری تجزیه کننده آن از سرده Acidovorax در 4 تیمار تحقیقاتی (کنترل، ترکیب دو باکتری تجزیه کننده، % 2 PHB و ترکیب دو باکتری + %2 PHB) بر فعالیت متابولیکی باکتری­های قابل کشت بی هوازی ، در بچه ماهیان تاسماهی سیبری با روش CLPP بررسی شد. نتایج نشان داد که پتانسیل فعالیت باکتری­های بی هوازی در تیمار2% PHB نسبت به سایر تیمارها متفاوت است و استفاده از این بیوپلاستیک باعث افزایش فعالیت متابولیکی باکتری­های بی هوازی در انتهای روده می شود. همچنین نتایج نشان داد که باکتری­های بی­ هوازی انتهای روده در بچه ماهیان تغذیه شده با این تیمار، دارای بالاترین فعالیت متابولیکی بر روی منابع کربنی اسید آمینه، کربوهیدرات و کربوکسیلیک اسیدها در میکروپلت ­ها بودند. نتایج این تحقیق نشان داد که استفاده از 2% PHB باعث افزایش فعالیت متابولیکی باکتری­های بی ­هوازی انتهای روده می شود و اضافه کردن باکتری­های تجزیه کننده آن بنا به دلایل نامعلوم تاثیری در افزایش این فعالیت ندارد.

کلیدواژه‌ها

اسیدهای چرب کوتاه زنجیره
بتابوتیریک اسید
بیولوگ اکوپلت
فلور میکروبی

عنوان مقاله English

The effects of the combination of bioplastic and its degrading bacteria (Genus Acidovorax) on the metabolic activity of anaerobic bacteria in Siberian sturgeon (Acipenser baerii) fingerlings hindgut by using CLPP

نویسندگان English

Ebrahim H. Najdegerami 1
Peter Bossier 2
1 Urmia University
2 Gent University
چکیده English

Community Level Physiological Profiles (CLPP) is novel method to evaluate microbial activity and diversity in ecosystems. According to the previous findings, poly-β-hydroxybutyrate (PHB) as a bio-control product, increases bacterial diversity in some aquatic animals. In this study, the effects of four experimental diets (control, combination of two PHB degrading bacteria, 2% PHB, bacteria+ 2% PHB) on the anaerobic activity of cultivable bacteria in Siberian sturgeon fingerlings hindgut was investigated. Community level physiological profile (CLPP) was used to analyze anaerobic metabolism by using Biolog™ Ecoplate microplates. The results indicated that anaerobic metabolic potential of the bacterial community was different in the 2% PHB-treated fishes as compared with other groups and PHB improved anaerobic metabolism of bacteria in fingerlings. Also anaerobic metabolism of bacteria was calculated on the different carbon sources (amino acids, carboxylic acids, carbohydrates and polymers) in Ecoplate microplates and the results showed that fish treated with PHB had the highest metabolic activity in mentioned carbon sources. The results of this research revealed that replacing of the diets with 2% PHB increases anaerobic metabolism of culturable bacteria in Siberian sturgeon hindgut while adding PHB degrading bacteria did not change this parameter in fingerlings.




کلیدواژه‌ها English

Short chain fatty acids
beta- hydroxybutyric acid
Biolog ecoplate
microbial community
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یافته های نوین در علوم زیستی
دوره 6، شماره 2 - شماره پیاپی 20
تابستان 1398
صفحه 169-175

  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405

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