یافته های نوین در علوم زیستی

بررسی اثرات آپوپتوزی نانو ذرات نقره با پوشش عصاره گیاه بوماردان بر سلول های سرطانی تخمدان A2780

نویسندگان

جواد بهارارا 1
طیبه رمضانی 2
نگار صغیری 1
فرزانه سالک 1

1 دانشگاه آزاد اسلامی واحد مشهد

2 دانشگاه خوارزمی

https://doi.org/10.29252/nbr.6.2.140
چکیده
استفاده از نانو ذرات نقرهبه علت ویژگی های منحصر به فرد آن در حال افزایش است. گیاه دارویی بومادران غنی از ترکیبات فعال زیستی می باشد. هدف از این پژوهش بررسی اثر ضد سرطانی نانو ذرات نقره سنتز شده با استفاده از عصاره بومادران بر رده سلولی سرطان تخمدان A2780 بود. اثر سمیت نانو ذرات نقره با آزمون MTT در زمان 48 ساعت و با غلظت های 2،4، 6، 8، 16 و 32 µg/ml بررسی گردید. برای مطالعه مسیر القاء مرگ سلولی از رنگ امیزی DAPI، اکریدین اورنج/ پروپودیوم یدید و آزمون انکسین 5/ پروپودیوم یدید و ارزیابی فعالیت کاسپاز 3 و 9 استفاده شد. نتایج : یافته ها نشان داد که نانو ذره نقره سنتز شده از گیاه بومادران در غلظت 4 میکروگرم بر میلی لیتر منجر به کاهش پنجاه درصدی زیست پذیری سلولی می گردد. همچنین نتایج حاصل از رنگ امیزیDAPI، اکریدین اورنج/ پروپودیوم ایداید، انکسین/ پروپودیوم یدید نشان دادکه درصد سلول های اپوپتوتیک در گروههای تیمار درمقایسه با کنترل افزایش یافته است. بعلاوه در سلول های تیمار شده فعالیت کاسپاز 3/9 افزایش یافت که نشان دهنده القای مرگ سلولی توسط نانو ذرات نقره از طریق مسیر وابست به کاسپاز می باشد. با توجه به نتایج حاصله می توان گفت که نانو ذرات سنتز شده با عصاره گیاه بومادران می تواند از طریق القاء آپوپتوز اثر سیتوتوکسیک خود را بر سلول های A2780 اعمال کند.

کلیدواژه‌ها

نانو ذرات
تولید زیستی
گیاه دارویی

عنوان مقاله English

Investigation apoptotic effects of silver nanoparticles coated with Achillea biebersteinii extract on A2780 ovarian cancer cells

نویسندگان English

J-avad Baharara 1
Tayebe Ramezani 2
Negar Saghiri 1
Farzane Salek 1
1 Mashhad Branch, Islamic Azad University,
2 Kharazmi University
چکیده English

In recent years using of silver nanoparticles due to its unique properties was increased. Medical plant, the Achillea biebersteinii is rich in anti-cancers compounds. The aim of this study was to investigate the cytotoxicity effects of AgNPs synthesized using Achillea biebersteinii extract on human ovarian cancer A2780 cells. Cytotoxic effects of AgNPs with MTT test was performed at 48 hours in concentration of 2, 4, 6, 8, 16 and 32 µg/ml. To study the cell death induced by AgNPs DAPI, acridine orange (AO)/ Propidium iodide (PI) staining and Annexin v/ Propidium iodide assay and activation of caspase 3 and 9 were assessed. Results: The results showed that the synthesized AgNPs decreased cell viability dose dependently, calculated inhibitory concentration of 50 % (IC50) was 4 µg/ml. The results from AO / PI, DAPI staining, Annexin V / PI showed that in treated cells the percentage of apoptotic cells compared with control increased. In addition, the treated cells showed increased activation of caspase 3/9. Hence silver nanoparticles induced cell death through the caspase dependent pathway. The results suggest that AgNPs synthesized using Achillea biebersteinii extract exert their cytotoxic effect by inducing apoptosis.

کلیدواژه‌ها English

Nanoparticles
Green Synthesis
Medical plant
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یافته های نوین در علوم زیستی
دوره 6، شماره 2 - شماره پیاپی 20
تابستان 1398
صفحه 140-147

  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405

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