مطالعه تاثیرعوامل محیطی و منابع کربن مختلف بر روی گوگردزدایی دی بنزوتیوفن توسط قارچ Exophiala spinifera

نویسندگان

دانشگاه اصفهان

چکیده
از آن جایی که کیفیت سوخت‌های فسیلی اثر مستقیمی بر سلامت محیط زیست دارد ضروری است که مقدار گوگرد موجود در این سوخت­ ها کاهش یابد. در این تحقیق سویه جدیدی از قارچ Exophiala spinifera به نام سویه FM استفاده شد که قادر به کاهش دی‌بنزوتیوفن (DBT) به عنوان یک مدل از ترکیب‌های گوگردی حلقوی موجود در سوخت‌های فسیلی بود. با انجام تحلیل HPLC مشاهده شد که قارچ مورد نظر طی 7 روز قادر به کاهش %99 از غلظت DBT در محیط کشت پایۀ نمکی است. این قارچ DBT را به­ مثابۀ منبع گوگرد و بصورت کومتابولیسم با سایر منابع کربنی مانند گلوکز مصرف می‌کند. قارچ E. spinifera در منابع کربنی مختلف شامل گلوکز، سوکسینات، گلوکز و اتانول به همراه DBT به عنوان منبع گوگرد کشت داده شد و بیش ترین رشد و فعالیت گوگردزدایی پس از 96 ساعت در حضور گلوکز به عنوان منبع کربن به دست آمد. در غلظت‌های مختلف DBT بهترین رشد و فعالیت گوگردزدایی در غلظت 3/0 میلی ­مولار مشاهده شد. بیشترین میزان گوگرددزایی DBT و رشد قارچ فوق در 26 تا 30 درجه سلسیوس مشاهده گردید. pH مناسب برای رشد ماکزیمم و بهترین فعالیت گوگردزدایی حدود 4 تا 5 به ­دست آمد.

کلیدواژه‌ها


عنوان مقاله English

Study the effect of environmental factors and different carbon sources on dibenzothiophene desulfurization by Exophiala spinifera

نویسندگان English

Fatemeh Elmi
Zahra Etemadifar
Giti Emtiazi
University of Isfahan
چکیده English

It is necessary to reduce the amount of sulfur in fossil fuels due to direct impact of the quality of these fuels on the environment. In this research, a novel fungus strain of Exophiala spinifera, namely FM, was used to desulfurize dibenzothiophene (DBT) as a model cyclic sulfur compounds in oil and fossil fuels. HPLC analysis indicated that the fungus was capable of reducing 99% of DBT concentration in BSM medium after seven days. This fungus utilized DBT as a sulfur source by co-metabolism reaction with other carbon sources such as glucose. Exophiala spinifera was inoculated in BSM medium containing DBT with various carbon sources including ethanol, glucose, succinate, and glycerol. This fungus had the highest growth and desulfurization capability on glucose as a carbon source after 96 h. E. spinifera had best growth and desulfurization rates in 0.3mM DBT. Optimum DBT desulfurization and growth rate of this fungus was observed at 26-30 oC. Suitable pH for the optimum growth and desulfurization activity of E. spinifera strain FM ranged 4-5.

کلیدواژه‌ها English

biodesulfurization
co-metabolism
Fungus
Optimization
organic sulfur
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  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405