بررسی ا‌ثر سالیسیلیک اسید بر فعالیت آنتی اکسیدانی نوشاخه های تکثیر شده گیاه بادرنجبویه (Melissa officinalis) درکشت درون شیشه ای

نویسندگان

1 دانشگاه الزهرا

2 دادانشگاه الزهرا

چکیده
بادرنجبویه گیاهی است دارویی از تیره نعناعیان که به علت خواص درمانی خود در بسیاری از نقاط دنیا کشت می شود. این پژوهش به منظور بهینه سازی فعالیت آنتی اکسیدانی در نوشاخه های تکثیر یافته در کشت بافت انجام شد ،بذرهای این گیاه در محیطMS بدون هورمون برای تولید گیاهجه کشت شدند. سپس نوشاخه های حاصل به محیط کشت MS حاوی mg/l2 BAPمنتقل شدند. پس از 45 روز سالیسیلیک اسید با غلظت های0، 50، 100 و 200 میکرومولار به محیط کشت اضافه شد. میزان فعالیت آنتی اکسیدانی و مقادیر ترکیبات فنلی، فلاونوئیدی و رزمارینیک اسید نوشاخه هادر فواصل زمانی 4, 7, 10و 14 روز پس از تیمار اندازه گیری شد. نتایج نشان داد کمترین میزان IC50 ، بیشترین فعالیت آنتی اکسیدانی با قدرت کاهشی و بیشترین میزان ترکیبات فنلی و رزمارینیک اسید در نوشاخه های تیمار شده با غلظت 100 میکرومولارسالیسیلیک اسید به مدت 14 روز مشاهده شد. همجنین بیشترین میزان ترکیبات فلاونوئیدی و بیشترین میزان فعالیت آنیون سوپراکسید در غلظت 50 میکرومولارسالیسیلیک اسید در روز 14 بدست آمد. به طورکلی غلظت 100 میکرومولارسالیسیلیک اسید به مدت 14 روز برای افزایش فعالیت آنتی اکسیدانی و ترکیبات دکر شده مناسب تر تشخیص داده شد.

کلیدواژه‌ها


عنوان مقاله English

Effect of salicylic acid on antioxidant properties of In vitro proliferated shoots of Melissa officinalis L.

نویسندگان English

Maryam ٍEbrahimi 1
Khadijeh Kiarostami 1
Zahra Nazem Bokaee 2
1 Alzahra University,
2 Alzahra University,
چکیده English

Melissa officinalis is a medicinal plant belong to lamiacea family. This plant has been cultivated in many parts of the world due to its therapeutic effects. This study was conducted to improve antioxidant activities of proliferated shoots from tissue culture. The seeds of plant were cultured in MS hormone free medium in order to obtain seedlings. Then the shoots were transfer to MS medium supplemented with 2 mg/ L BAP.After 45 days salicylic acid (SA) was added to the medium at concentrations of 0, 50, 100 and 200 µM. The antioxidant activity and phenolics, flavonoids and rosmarinic acid content in proliferated shoots were evaluated 4,7,10 and 14 days after treatment. As a result the highest free radical scavenging and reducing power activity, as well as the highest value of total phenolic and rosmarinic acid were observed in shoots after 14 days of treatment with 100 µM salicylic acid. The elevated level of superoxide anion scavenging activity and total flavonoids were obtained from the shoots treated with 50 µM salicylic acid for 14 days. Over all treatment with100 µM salicylic acid for 14 days was better in order to antioxidant activity and compounds with recognized antioxidant activity.

کلیدواژه‌ها English

antioxidants
phenolics
rosmarinic acid
flavonoids
salicylic acid
Melissa Officinalis
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  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405