بررسی تنوع ژنتیکی دو گونه براق Ganoderma lucidum و Ganoderma resinaceum به وسیله نشانگر RAPD

نویسندگان

1 دانشگاه فرهنگیان، تهران،

2 دانشگاه شهید بهشتی،

3 دانشگاه شهید مدنی آذربایجان

4 مرکز تحقیقات کشاورزی و منابع طبیعی مازندران

5 موسسه تحقیقات گیاه­پزشکی کشور

6 دانشگاه تربیت مدرس

چکیده
سرده .Ganoderma Karst متعلق به راسته Polyporales بوده و از قارچ های مهم دارویی و بیماری زای گیاهی است. هدف از انجام این تحقیق بررسی تنوع ژنتیکی جمعیت های طبیعی دو گونه براق Ganoderma با استفاده از نشانگر مولکولی RAPD است. بدین منظور چهار جمعیت از گونه .Ganoderma resinaceum Boud و هشت جمعیت از گونه .Ganoderma lucidum Karst جمع آوری شده از جنگل های شمال کشور طی سالهای 91 - 90 برای اولین بار در ایران مورد بررسی قرار گرفت. برای تکنیک RAPD، از 10 آغازگر تصادفی استفاده شد. نتایج RAPD – PCR موید وجود تنوع ژنتیکی وسیعی بین جمعیت های مورد مطالعه بود به طوری که تنوع درون گونه ای G. lucidum و G. resinaceum به ترتیب 48 / 61 و 16 / 40 برآورد شد. نتایج حاصل از تجزیه خوشه ای نشان داد که جدایه ها در سه خوشه قرار گرفتند و این گروه بندی جدایی جمعیت های دو گونه مورد مطالعه را تأیید کرود. در ارزیابی تجزیه ساختار ژنتیکی، دو گروه احتمالی (K=2) در ژرم پلاسم مورد مطالعه شناسایی شد به طوری که نتایج حاصل از بارپلات نیز آن را تأیید کرد.

کلیدواژه‌ها


عنوان مقاله English

The Study of the genetic diversity of two laccate species of Ganoderma lucidum and Ganoderma resinaceum using RAPD marker

نویسندگان English

Somayeh Keypour 1
Hossein Riahi 2
Mostafa Ebadi 3
Ali Borhani 4
Mohammad Reza Asef Shayan 5
Naser Safaie 6
چکیده English

The genus Ganoderma belongs to Polyporales, well-known for its medicinal properties and as a plant pathogen. The aim of this study was to identify the genetic variation between populations of two Ganoderma lucidum Karst. complex isolates using RAPD. For this purpose, four populations of G. resinaceum Boud. and eight populations of G. lucidum collected and purified during 2011-2012, were studied for the first time in Iran. Ten random primers were used for RAPD reaction. The result for RAPD-PCR studies of G. lucidum complex showed a wide range of diversity, as scores of 61.48 and 40.16 were estimated for the inter-specific diversity of G. lucidum and G. resinaceum, respectively. Cluster analysis confirmed the separation of two species populations in different clusters. In the analysis of genetic structure, two probable groups (K = 2) were identified in the studied germplasm which was also confirmed by the results of bar plot.

کلیدواژه‌ها English

cluster analysis
genetic structure analysis
genetic variation
germplasm
random primers
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  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405