اثر ضدافسردگی عصاره هیدروالکلی چویل در مدل ایسکمی/هایپوپرفیوژن در موش صحرایی نر ویستار بالغ

نویسندگان

1 دانشگاه آزاد اسلامی واحد ایذه

2 دانشگاه آزاد اسلامی دامغان

3 گروه زیست شناسی، دانشگاه آزاد اسلامی واحد گمیشان، مرکز گرگان ، گرگان، ایران

چکیده
در بیماران مبتلا به ایسکمی مغزی، چه در زمان بستری و چه در جامعه، شیوع افسردگی ماژور مشهود است از آنجا که افسردگی بر روی بهبود فرد تأثیر منفی دارد، تشخیص و درمان به­موقع آن ضروری است. این مطالعه به تأثیر عصاره­ی هیدروالکلی چویل بر شاخص­ های استرس­اکسیداتیو مغز و افسردگی پس از انسداد دوطرفه دائم شریان کاروتید مشترک یا همان ایسکمی/هایپوپرفیوژن در موش صحرایی های نر بالغ می پردازد. تعداد 35 موش صحرایی نر نژاد ویستار به گروه های کنترل، ایسکمی و گروه ­های ایسکمی دریافت­ کننده 14 روزه دوزهای (100، 200 و 400 mg/kg) عصاره چویل به روش گاواژ تقسیم شدند. برای ایجاد مدل حیوانی از ایسکمی/هایپوپرفیوژن مغزی دائم، شریان های کاروتید به فاصله یک هفته از بالا و پایین بسته شده و برش یافتند. برای سنجش افسردگی زمان بی­حرکتی در شنای اجباری اندازه ­گیری شد و سپس بافت مغز برای تفکیک هیپوکامپ و اندازه­ گیری مالون­دی­الدئید خارج شد. در گروه ایسکمی/هایپوپرفیوژن، زمان بی­حرکتی و مالون­دی­الدئید افزایش معنی­ داری نشان داد و گاواژ هر سه دوز عصاره چویل به موش­های ایسکمی باعث کاهش قابل­ توجه غلظت مالون دی الدئید در هیپوکامپ و نیز کاهش زمان بی­حرکتی به­ ترتیب در دوز 100 و دوزهای mg/kg 200 و 400 گردید و افزایش زمان شنا در هرسه گروه دریافت کننده­ی عصاره مشاهده شد. عصاره چویل با اثر آنتی اکسیدانی می تواند از عوارض ناشی از ایسکمی از جمله افسردگی را بکاهد.

کلیدواژه‌ها


عنوان مقاله English

Anti-depressive effects of hydro-alcoholic extract of Chevilan (Ferulago angulate Boiss.) in adult male wistar rat model of ischemia/ hypoperfusion

نویسندگان English

Maryam Rafieirad 1
Zeinab Eydipour 2
Shahrbanoo Alami Rostami 3
1 Department of Biology, Faculty of Sciences, Izeh Branch, Islamic Azad University, Izeh, Iran
2 Damghan I A U
3 Department of Biology,Islamic Azad Univercity,Gomishan Branch, Gorgan Center,Gorgan Iran
چکیده English

In patients with cerebral ischemia, both during hospitalization and in the community, the prevalence of major depression is evident. Since the depression has a negative impact on recovery, its timely diagnosis and treatment is essential. This study aimed to evaluate the effect of the oral administration of Chevilan extract (Ferulago angulata hydroalcoholic extract; 100, 200 and 400 mg/kg) on brain oxidative stress indices and depression after permanent bilateral common carotid artery occlusion or ischemia/hypoperfusion in male adult rats. A number of 35 rats were divided into a control group, an ischemic group and ischemic groups receiving doses of 100, 200 and 400 mg/kg of Chevilan extracts for 14 days by gavage. To make animal models of permanent cerebral hypo perfusion/ischemia, right common carotid artery was ligatured first and the left one ligatured with an interval of one week. To evaluate depression, immobility in the forced swimming time of each rat was measured and then the rat’s brain tissues were extracted to separate hippocampus and measure malondialdehyde. The results showed that ischemia/hypoperfusion increased brain oxidants such as lipid per oxidation (LPO) and immobility. Also, ischemic rats treated with all three doses of the Chevilan show significant reduction in the concentration of MDA hippocampus and in immobility time at a dose of 100 mg/kg and 200 / 400mg, respectively. Increased swimming time was observed in all three groups of extract recipients. It was found that Chevilan extract with antioxidant effect can reduce the side effects of ischemia such as depression.

کلیدواژه‌ها English

carotid artery occlusion
forced swimming
herbal extract
malondialdehyde
oxidative stress
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دوره 5، شماره 2 - شماره پیاپی 16
تابستان 1397
صفحه 137-143

  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405