اثر حمایتی گیاه باباآدم بر استرس اکسیداتیو و مسمومیت القاشده با جنتامایسین

نویسندگان

دانشگاه بوعلی سینا، همدان

چکیده
هدف این مطالعه بررسی اثر حمایتی عصارۀ هیدرواتانولی بخش هوایی (برگ و ساقه) گیاه باباآدم بر مسمومیت کلیوی القاشده رَت­ها با جنتامایسین بود. 24 سر رَت­ نژاد ویستار به چهار گروه تقسیم شدند. که شامل: گروه کنترل، گروه جنتامایسین (تزریق درون­ صفاقی 100 میلی­گرم به­ ازای هر کیلوگرم وزن بدن)، گروه جنتامایسین+عصاره (تزریق درون­ صفاقی جنتامایسین و دریافت دهانی 500 میلی­گرم به ­ازای هر کیلوگرم وزن بدن) و گروه عصاره (دریافت دهانی 500 میلی­گرم عصاره به­ ازای هر کیلوگرم وزن بدن). طول دورۀ تیمار ده روز بود. مسمومیت کلیوی به ­روش بیوشیمیایی و هیستولوژیکی بررسی شد. غلظت کراتینین، اوره، مالون دی­آلدهید، سوپراکسید­دیسمیوتاز و پراکسید­هیدروژن خون تعیین شد. به­ علاوه، بررسی ­های هیستولوژیکی نیز انجام شد. غلظت اوره، کراتینین، مالون دی­آلدهید و پراکسید هیدروژن در سرم خون حیوانات تیمارشده با جنتامایسین به­ طور معنی­ داری بالا و فعالیت سوپراکسید­دیسمیوتاز به­ طور معنی ­داری پایین بود. درحالی­ که تیمار هم­زمان عصاره شاخص­ های بیوشیمیایی مسمومیت کلیوی را کاهش داد. بررسی هیستولوژیکی نکروز و ریزش سلول ­های اپی­تلیالی لوله­ های پیچیدۀ نزدیک واقع در بخش قشری کلیۀ حیوانات تیمارشده با جنتامایسین را نشان داد. درحالی­ که تیمار هم­زمان عصاره با جنتامایسین، تخریب بافتی کمتری را نشان داد. نتایج حاکی است که تیمار با باباآدم می­تواند مسمومیت القاشده با جنتامایسین را کاهش دهد.

کلیدواژه‌ها


عنوان مقاله English

Protective effect of Arctium lappa on oxidative stress and nephrotoxicity induced by gentamicin

نویسندگان English

Siamak Yari
Roya Karamian
Mostafa Asabbeigi
Ali Namdari
Bu-Ali Sina University, Hamedan
چکیده English

This study aimed to investigate the protective effect of Arctium lappa (AL) on gentamicin (GM)-induced nephrotoxicity in rats. Twenty-four Wistar rats were divided into four groups including: control group; GM group (intrapritoneal injection, IP, of 100 mg/kg GM B.W.); GM+AL group (received IP injection of 100 mg/kg GM and 500 mg/kg AL orally) and AL group (received 500 mg/kg AL orally). The experimental period lasted for 10 days. Nephrotoxicity was biochemically and histologically evaluated. The concentrations of creatinine, urea, malondialdehyde (MDA), superoxide dismutase (SOD) and peroxide hydrogen (H2O2) in the serum samples were determined. Moreover, histological examinations were performed. The animals treated with gentamicin showed significantly higher serum urea, creatinine, MDA and H2O2 levels and lower SOD activity. However, co-administration of AL produced amelioration in biochemical indices of nephrotoxicity in serum. Histomorphological examination showed necrosis and desquamation of tubular epithelial cells in the renal cortex in animals treated with gentamicin whereas simultaneous administration of AL and GM reduced histological damages. The data obtained suggest that treatment with AL extract can help to reduce gentamicin-induced nephrotoxicity.




کلیدواژه‌ها English

creatinine
hydrogen peroxide
Kidney tissue
malondialdehyde
rat
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  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405