اثر متقابل تنش خشکی و نیترات پتاسیم بر برخی پاسخ های فیزیولوژیک گیاه توتون (.Nicotiana tabacum L)

نویسندگان

گروه زیست شناسی، دانشکدۀ علوم پایه، دانشگاه گیلان، رشت

چکیده
در این تحقیق تنش خشکی ازطریق پلی­اتیلن گلیکول با غلظت 20 درصد اعمال شد. 48 ساعت پس ­از اعمال تنش و به­منظور ارتقای مقاومت دربرابر تنش، تیمار نمونه­ ها به­ وسیلۀ نیترات­ پتاسیم در سه غلظت 5، 10، 15 میلی­ مولار طی 9 روز انجام گرفت. تغییرات مقدار پرولین، پروتئین کل، رنگیزه­ های فتوسنتزی، بتاکاروتن، آنتوسیانین، مالون­ دی­ آلدهید، فنل، فلاونول، فلاونوئید، قندهای محلول و پتاسیم برگ اندازه­ گیری شد. نتایج نشان داد که گیاه توتون در مواجه با تنش خشکی به­ منظور حفظ فشار اسمزی داخلی، از تجمع اسمولیت ­هایی مانند پرولین، قندهای محلول و پتاسیم کمک می ­گیرد. همچنین تنش خشکی باعث ایجاد تنش اکسایشی در گیاه توتون می­ شود و تولید فرم­های فعال اکسیژن را افزایش می ­دهد. درنتیجه سیستم دفاع آنتی ­اکسیدانی غیرآنزیمی گیاه توتون شامل آنتوسیانین، فلاونوئید، فلاونول و بتاکاروتن برای مقاومت دربرابر خشکی افزایش می ­یابد. نتایج همچنین نشان داد که کاربرد نیترات پتاسیم به ­ویژه غلظت 15 میلی­ مولار به طور محسوسی توانست برخی از پیامدهای مضر تنش، همچون کاهش رنگیزه ­های فتوسنتزی و پروتئین را بهبود ببخشد. همچنین نیترات پتاسیم توانسته سطوح MDA و بتاکاروتن را به سطح معادل آن در گیاهان کنترل برساند. درنتیجه به­ نظر می ­رسد کاربرد پتاسیم می­ تواند در مقاومت گیاه به خشکی مؤثر باشد و در کاهش برخی اثرهای مضر تنش خشکی نقش مهمی را ایفا ­کند.

کلیدواژه‌ها


عنوان مقاله English

The effect of the interaction between water stress and potassium nitrate on some of the physiological responses of Nicotiana tabacum L.

نویسندگان English

Akbar Norastehnia
Maliheh Farjadi
Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran
چکیده English

In this study, water stress was applied by polyethylene glycol at a concentration of 20 perecentage. To improve the resistance of the plants, the samples were treated by potassium nitrate at concentrations 5, 10, and 15 mM within 9 days. Changes in proline, total protein, photosynthetic pigments, carotene, anthocyanin, malondialdehyde, phenols, flavonols, flavonoids, soluble sugars and potassium ion were examined. The results showed that tobacco plants which had been exposed to drought used the accumulation of osmolytes such as proline, soluble sugars and potassium in order to balance their osmotic pressure. Drought stress also caused oxidative stress and increased the production of active forms of oxygen. As a result, non-enzymatic antioxidant defense system of tobacco plants including anthocyanins, flavonoids, flavonols and beta-carotene increased, which could be considered to be a major step for resistance to drought. The results also showed that the concentration of 15 mM potassium nitrate in particular, could significantly improve some of the harmful effects of stress and reduced photosynthetic pigments and proteins. Potassium nitrate could also bring down the MDA and beta-carotene levels to equivalent levels in control plants. As a result, it seems that using potassium can affect plant resistance to drought and plays an important role to reduce some harmful effects of stress.

کلیدواژه‌ها English

nonenzymatic antioxidants
polyethylene glycol
drought stress
malondialdehyde
potassium nitrate
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  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405