مطالعۀ اثر میدان مغناطیسی و سالیسیلیک اسید بر گیاه بادرنجبویه (نعناعیان) تحت تنش فرابنفش B

نویسندگان

دانشگاه ارومیه،

چکیده
اثرات ناشی از کاهش لایۀ اوزون و افزایش اشعۀ فرابنفش را امروزه بسیاری از محققان بررسی کرده­اند. هدف این مطالعه، بررسی تأثیر پرتو UV-B بر گیاه بادرنجبویه (ملیسا اوفیسینالیس) و تأثیر سالیسیلیک اسید و میدان مغناطیسی در کاهش اثر زیان­بار این پرتو بر این گیاه بود. گیاهان بادرنجبویه به مدت 60 روز در وضعیت گلخانه­ای کشت یافتند. تیمار فرابنفش بعد از مرحلۀ شش­برگی اعمال شد و پرتو UV-B به مدت 15 روز و هر­بار به مدت 20 دقیقه اعمال گردید. اسید سالیسیلیک نیز بعد از مرحلۀ شش­برگی با غلظت 1 میلی­مولار به مدت یک­هفته روی گیاهان پاشیده شد. تیمار میدان مغناطیسی به مدت یک ساعت و با شدت­های صفر، 40 و 85 میلی­تسلا اعمال شد. نتایج نشان داد که پرتو UV-B باعث کاهش وزن تر و خشک، رشد طولی ریشه و اندام هوایی شد. این پرتو همچنین میزان رنگیزه­های فتوسنتزی (کلروفیل a، کلروفیل b و کاروتنوئید)، میزان قندها و پروتئین­های محلول را کاهش داد. میزان فعالیت فنیل­آلانین آمونیا­لیاز و ترکیبات جاذب UV نیز تحت تأثیر پرتوهای UV-B افزایش نشان داد. بررسی نتایج نشان داد تیمار با سالیسیلیک اسید و میدان مغناطیسی باعث تخفیف آسیب­های ناشی از اعمال پرتوهای UV-B در گیاه شد و توانست فاکتورهایی را که تحت تابش­های فرابنفش تغییر یافته بودند جبران کند.

کلیدواژه‌ها


عنوان مقاله English

Investigating the effects of magnetic field and salicylic acid on Melissa officinalis (Lamiaceae) under UV-B stress

نویسندگان English

Latifeh Pourakbar
Mehdi Abedzadeh
Urmia University
چکیده English

Effects of ozone depletion and increase of UV radiation have been studied by many researchers. The aim of this study was to investigate the effects of UV-B radiation on Melissa officinalis L. as well as the impact of salicylic acid and magnetic fields in the reduction of harmful effects of radiation on plants. The Melissa officinalis L. plants were treated with 28 ± 2 Cº for approximately 60 days. The light intensity was 150 µ E mˉ² Sˉ¹ and the light/dark regime was 16/8 h. UV-B treatment was applied after the six-leaf stage for a period of 15 days and 20 min every day. Salicylic acid (1mM) was daily sprayed on the plants after the six-leaf stage for one week. Magnetic field treatment was applied for an hour with intensities of 0, 40 and 85 mT. The results indicated that the UV-B radiation led to reduction of root and shoot dry and fresh weights, and root and shoot elongation. The radiations also reduced the content of photosynthetic pigments (chlorophyll a, chlorophyll b and carotenoid), sugar and soluble proteins. The phenylalanine ammonia-lyase activity and UV-absorbing content compounds increased under the effect of UV-B radiation. The results showed that treatment with salicylic acid and magnetic fields reduced damages to the plant due to UV-B radiation.

کلیدواژه‌ها English

UV radiation
Melissa officinalis L
magnetic field
salicylic acid
phenylalanine ammonia-lyase
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  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405