اثر تزریق داخل پوستۀ هستۀ اکومبنسی اسیدآسکوربیک بر یادگیری و حافظۀ فضایی موش های صحرایی نر بالغ

نویسندگان

دانشگاه شهید باهنر کرمان

چکیده
اسید­آسکوربیک به ­منزلۀ یک ویتامین و تعدیل­ کنندۀ عصبی در بیشتر نواحی مغز پستانداران از­جمله پوستۀ هستۀ اکومبنس یافت می­شود. در تحقیق حاضر به بررسی نقش اسید­آسکوربیک در پوستۀ هستۀ اکومبنس بر حافظۀ فضایی و یادگیری موش ­های صحرایی پرداختیم. به این منظور از 35 سر موش صحرایی نر از نژاد ویستار در محدودۀ وزنی 270-220 گرم در 5 گروه استفاده شد: گروه کنترل، شاهد و سه گروه دریافت­کنندۀ دوزهای (12، 24 و 48 μg/rat/side ) اسید­اسکوربیک. یک­ هفته پس از بهبودی تزریق به داخل پوستۀ هستۀ اکومبنس صورت گرفت. سی­ دقیقه پس ­از تزریق، حیوان در ماز آبی موریس (MWM) قرار گرفت و شاخص ­های یادگیری و حافظۀ فضایی ثبت و تحلیل شد. نتایج نشان داد اسید­ اسکوربیک به طور معنی­ داری در دوز μg/rat/side 48 مسافت طی­شده و زمان رسیدن به سکو پنهان را در مقایسه با کنترل شاهد افزایش می­ دهد (001/0p<). تزریق داخل پوستۀ هستۀ اکومبنس اسید­اسکوربیک به نقص یادگیری فضایی انجامید.

کلیدواژه‌ها


عنوان مقاله English

The effect of intra-nucleus accumbens shell injection of ascorbic acid on spatial learning and memory in adult male rats

نویسندگان English

Fateme Shahsavari
Mahdi Abbasnejhad
Reihane Naderi
Saeed Esmaeeli Mahani
Shahid Bahonar University
چکیده English

Ascorbic acid (AA) as a vitamin and neuromodulator is present in most parts of CNS such as nucleus accumbens shell (Acbsh). This study evaluates the effect of intra-nucleus accumbens shell injection of AA on spatial learning and memory. 35 adult male Wistar rats (220-270 g) were divided into 5 groups: control (intact), sham AA (injected normal saline as AA vehicle) as well as 3 groups of AA (12, 24 and 48 μg/rat/side). After one week of recovery injections were performed. Thirty minutes after each injection the rats were trained in the Morris Water Maze (MWM) and spatial learning and memory parameters were recorded and then analyzed. The results showed that AA at the dose of 48 μg/rat/side significantly increased the travelled distance and latency time to reach the hidden platform in comparison with either control or sham groups (p<0.001). Intra-Acbsh injection of AA led to deficiency of spatial learning.

کلیدواژه‌ها English

spatial learning and memory
ascorbic acid
nucleus accumbens shell
morris water maze
Abbasnejad, M., Nasri, S., Nazem, H. and Bahaaddini, M. 2008. The effect of ascorbic acid injection into the lateral ventricle on spatial learning and memory in adult male rats. – Physiology and Pharmacology 12: 227-237.
Adriani, W., Sargolini, F., Coccurello, R., Oliverio, A. and Mele, A. 2000. Role of dopaminergic system in reactivity to spatial and non-spatial changes in mice. – Psychopharmacology 150: 67-76.
Badreh, F., Abbasnejad, M., Derakhshani, A. and Jonaidi, H. 2009. Interaction between ascorbic acid and dopamine D2 receptor in the nucleus accumbens shell in response to feeding. – Int. J. Biol. Chem. 3: 132-141.
Castellano, C., Cestari, V. and Ciamei, A. 2001. NMDA receptors and learning and memory processes. – Curr. Drug Targets 2: 273-283.
Cools, A., Miwa, Y. and Koshikawa, N. 1995. Role of dopamine D1 and D2 receptors in the nucleus accumbens in jaw movements of rats: a critical role of the shell. – Eur. J. Pharmacol. 286: 41-47.
D’Hooge R. and De Deyn P. 2001. Applications of the Morris water maze in the study of learning and memory. – Brain Research Reviews 36: 60- 90.
Esmaili, M.H., Doodangeh, E. and Sharif, M. 2003. The effect of ascorbic acid on spatial learning. – The Journal of Qazvin Univ. of Med. Sci. 24: 3-8.
Essman, W.D., McGonigle, P. and Lucki I. 1993. Anatomical differentiation within the nucleus accumbens of the locomotor stimulatory actions of selective dopamine agonists and damphetamine. – Psychopharmacology 112: 233- 241.
Ferretti, V., Florian, C., Costantini, V.J.A., Roullet, P., Rinaldi, A., Leonibus, E.D., Oliverio, A. and Mele, A. 2005. Co-activation of glutamate and dopamine receptors within the nucleus accumbens is required for spatial memory consolidation in mice. – Psychopharmacology 179: 108-16.
Gu, P.F., Wu, C.F., Yang, J.Y., Shang, Y., Hou, Y., Bi, X.L. and Dai, F. 2006. Differential effects of drug induced ascorbic acid release in the striatum and the nucleus accumbans of freely moving rats. – Neurosci. Let. 33: 79-81.
Harrison, F.E., Hosseini, A.H., Dawes, S.M., Weaver, S. and May, J.M. 2009. Ascorbic acid attenuates scopolamine-induced spatial learning deficits in the water maze. – Behavioral Brain Res. 28: 550-558.
Ito, R., and Hayen, A. 2011. Opposing roles ofnucleus accumbens core and shell dopamine in the modulation of limbic information processing. – J. Neurosci. 31: 6001-6007.
Kumar, R.S., Narayanan, N. and Nayak, S.2009. Ascorbic acid protects against restraint stress-induced memory deficits in wistar rats. – Clinics 64: 1211-1217.
Liu, W., Wu, C.F., Huang, M. and Xiao, K. 2000. Opposite effects of sulpiride and SCH 23390 on ethanol-induced striatal ascorbic acid release in intact and 6-hydroxydopamine lesioned rats. – Brain Res. 869: 31-38.
Mele, A., Avena, M., Roullet, P., Leonibus, E.D., Mandillo, S., Sargolini, F., Coccurello, A. and Oliverio, A. 2004. Nucleus accumbens dopamine receptors in the consolidation of spatial memory. – Behav. Pharmacol. 15: 423-431.
Miquel, M., Aguilar, M.A. and Aragon, M.G.1999. Ascorbic acid antagonizes ethanol-induced locomotor activity in the open-field. – Pharmacol. Biochem. Behav. 62: 361-366.
Mogenson, G.J., Jones, D.L., Yim, C.Y. 1980. Frome motivation to action: functional interface between the limbic system and the motor system. – Prog. Neurobiol. 23: 69-97.
Morris, B.J. 1989. Neuronal localization of neuropeptide Y gene expression in rat brain. – J. Comp. Neurol. 290: 358-368.
Naderi, R. and Abbasnejad, M. 2013. Comparison of the effect of co-injection of ascorbic acid and D2 agonist (bromocriptine) with ascorbic acid in nucleus accumbens shell on male rats' anxiety. – J. Babol. Univ. Med. Sci. 15: 100- 108.
Nelson, A.J.D., Thur, K.E., Marsden, C.A. and Cassaday, H.J. 2010. Dissociable roles of dopamine within the core and medial shell of the nucleus accumbens in memory for objects and places. – Behavioral Neuroscienc 124: 789-799.

Prinssen, E.P.M., Balestra, W., Bemelmans, F.F. and Cools, A.R. 1994. Evidence for a role of the shell of the nucleus accumbens in oral behavior of freely moving rats. – J. Neurosci. 14: 1555-1562.
Ramírez-Lugo, L., Zavala-Vega, S. and Bermúdez-Rattoni, F. 2006. NMDA and muscarinic receptors of the nucleus accumbens
have differential effects on taste memory formation. – Learn. Mem. 13: 45-51.
Rebec, G.V. and Pierce, R.C. 1994. A vitamin as neuromodulator: ascorbate release into the extracellular fluid of the brain regulates dopaminergic and glutamatergic transmission. – Prog. Neurobiol. 43: 537-565.
Rice, M.E. 2009. Ascorbat regulation and its neuroprotective role in the brain. – Trends Neurosci. 23: 209-216.
Sahraei, H., Aliabadi, A.A., Zarrindast, M.R., Ghoshooni, H. and Barzegari-Sorkheh, A.B. 2007. Ascorbic acid antagonizes nicotine-induced place preference and behavioral sensitization in mice. – Eur. J. Pharmacol. 56: 42-48. Setlow, B. 1997. The nucleus accumbens and learning and memory. – J. Neurosci. Res. 49: 515- 521.
Shahsavary, F. and Abbasnejad, M. 2013. Effect of co-administration of ascorbic acid and bromocriptine in nucleus accumbens shell on locomotor activity in male rats by open field test. – Physiology and Pharmacology 17: 125-136.
Tolbert, L.C., Morris, P.E.J., Spollen, J.J. and Ashe, S.C. 1992. Stereo specific effects of ascorbic acid and analogues on D1 and D2 agonist binding. – Life Sci. 51: 921-930.
Tou, J.C. and Wade, C.E. 2002. Determinations affecting physical activity levels in animal models. – Exp. Biol. Med. 227: 587-600.

  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405