مطالعۀ باکتریایی ریشۀ ذرت (.Zea mays L) در خاک آلوده به نفت

نویسندگان

دانشگاه الزهرا

چکیده
های این ناحیه در افزایش تجزیۀ آلاینده­های متنوع آلی خاک نقش مهمی دارند. در این تحقیق به بررسی میکروبی ریشۀ گیاه ذرت پس­از طی یک دورۀ رشد 60 روزه در سه نوع خاک شاهد، آلوده به نفت و آلوده به نفت همراه با کود نیتروژنی پرداخته شد و باکتری­های ناحیۀ ریشه شناسایی شد. پس از تعیین مقاوم­ترین باکتریبه نفت خام، توانایی تولید بیوسورفکتانت در باکتری­های جدا­شده از خاک­های آلوده بررسی شد. نتایج شمارش تعداد باکتری­ها نشان داد که تعداد کل کلنی­های میکروبی خاک شاهد از دو تیمار دیگر بیشتر بود و در میزان تنوع میکروارگانیسم­ها نیز تفاوت مشاهده شد. مشخص شد که تمام باکتری­های جدا­شده از خاک­های آلوده توانایی تولید مقادیر مختلف بیوسورفکتانت را دارند.

کلیدواژه‌ها


عنوان مقاله English

The bacterial evaluation of maize root (Zea mays L.) in oil-polluted soil

نویسندگان English

Parisa Mohammadi
Sepide Ameri Toorzani
Khadije Kiarostami
Sara Gheravi
Zahra Felahti
Alzahra University
چکیده English

Total petroleum hydrocarbons (TPHs) is one of the most important environmental challenges. Phytoremediation of oil-polluted soil depends on microorganisms of the rhizosphere. This study attempts to evaluate the microbial impact of Zea mays rhizosphere during a 60 day period using three different treatments: control soil, oil-polluted soil and oil-polluted soil including fertilizer (NH4No3, K2HPO4). Then, the rhizosphere bacteria were identified. The results showed that CFU per gram of control soil was higher than other treated soils, an indication of oil stress on microbial population. In addition, more bacterial diversity was observed in soils and different bacteria were isolated from the soils. Finally, the most resistant bacteria to the oil pollution were assayed. The assay of isolates indicated that all of those separated from the oil-polluted soil have the ability to produce biosurfactant in different quantities. Further investigation must be carried out to optimize the degradation of oil by these isolates.

کلیدواژه‌ها English

phytoremediation
microorganism
biosurfactant
oil pollution
Poaceae
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  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405