بررسی تاثیر فولات بر خواص فیزیکوشیمیایی و ضد سرطانی نانوکپسول‌های بتالاکتوگلوبولینی بر روی رده سلول‌های سرطانی کولون

نویسندگان

1 گروه زیست شناسی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد کرج، کرج، ایران

2 گروه علوم سلولی و مولکولی، دانشکده علوم زیستی، دانشگاه خوارزمی، تهران، ایران

چکیده
سیستم‌های دارورسانی مبتنی بر نانوتکنولوژی دارای پتانسیل بسیار مناسبی در کاربرد‌های پزشکی و درمانی هستند. لذا در این پژوهش طراحی و مشخصه‌یابی نانودارویی با پوشش پروتئین بتالاکتوگلوبولین حاوی اگزالی پالادیوم در حضور و غیاب فولات بررسی شده است .. همچنین خواص ضدسرطانی این نانوداروی هدفمند با فولات علیه سلول‌های سرطان کولون مطالعه شدند. تکنیک‌های پراکندگی دینامیکی نور، میکروسکوپ الکترونی روبشی و جذب اتمی به منظور تعیین ویژگی‌های نانوکپسول سنتز شده به کار گرفته شدند. سپس جهت بررسی سمیت سلولی و مکانیسم القای مرگ نانو دارو بر روی رده سلول‌های سرطانی کولونHCT116 از تستهای MTT و فلوسایتومتری استفاده شد. نتایج آزمایش­های پراکندگی نور دینامیک، میکروسکوپ الکترونی پویشی و میکروسکوپ نیروی اتمی نشان داد نانوکپسول‌های حاوی دارو و هدفمند با فولات اندازه‌ای حدود40 نانومتر و ساختار کروی شکل دارند. نتایج سنجش MTT و فلوسایتومتری بیانگر آن بود که نانو دارو هدفمند شده با فولات در الگوی وابسته به زمان و دوز، بقا و تکثیر را در سلول‌های HCT116 القا می‌کند. نتایج نشان داد که فولیک اسید قادر است اثر بخشی دارو نسبت به داروی بدون فولات بر سلول‌های سرطانی را افزایش دهد که می‌تواند به‌عنوان گزینه‌ای جدید در روش‌های تولید داروهای جدید در درمان سرطان مطرح گردد.





کلیدواژه‌ها


عنوان مقاله English

Investigation of presence of folate on physicochemical and anticancer properties of beta-lactoglobulin nanocapsules against human colon cancer cell line of HTC116

نویسندگان English

Mahdieh Garshasbi 1
Adeleh Divsalar 2
1 Isalmic Azad University
2 Kharazmi University
چکیده English

Nanotechnology-based targeting drug delivery systems have a considerable potential in medicine. Therefore, the present study aimed to designe, synthesise and characterize a nanodrug with beta lactoglobulin coating including oxali-palladium with and without folate and to compare their anti-cancer effects. The physicochemical properties of nanocapsules were studied by Dynamic light scattering (DLS), atomic force microscopy (AFM) and scanning electron microscopy (SEM). Finally, the anticancer activities of nanodrugs were investigated against human colorectal cancer cell line of HCT116 by MTT and flowcytometry methods. The results of Dynamic light scattering (DLS), scanning electron microscopy (SEM) and atomic force microscopy (AFM) showed that the average size of nanocapsules with folate were less than 40 nm. Cytotoxicity results proved the dose- and time-dependent antiproliferation and anicancer activities of nanocapsules (with folate) against HCT116. Finally, it could be concluded that folate increase anticancer activity of nanodrugs and it might be considered as a new candidate in the design and synthesis of new drugs in cancer treatment.




کلیدواژه‌ها English

beta lactoglobulin protein
Hct116
folate
Pectin
nanodrug
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  • تاریخ دریافت 20 تیر 1405
  • تاریخ انتشار 20 تیر 1405