fa اثرات آستاگزانتین بر اختلالات یادگیری و حافظه و آسیب اکسیداتیو القا شده با اتانول در ناحیه هیپوکامپ موش کوچک آزمایشگاهی علوم جانوری Animal Biology مقاله پژوهشی Original Article <span style="font-size:11pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">مصرف طولانی مدت اتانول با القای استرس اکسیداتیو، نورون‌های سیستم عصبی مرکزی و سلول‌های هیپوکامپ را از بین می‌برد. آستاگزانتین (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">ATX</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">) کاروتنوئیدی آنتی‌اکسیدان و ضدالتهاب است. این مطالعه با هدف بررسی اثرات آستاگزانتین بر اختلالات یادگیری و حافظه و آسیب اکسیداتیو ناشی از اتانول در هیپوکامپ <span style="background:yellow">موش کوچک آزمایشگاهی</span> انجام شد. 35 سر <span style="background:yellow">موش کوچک آزمایشگاهی</span> به پنج گروه تقسیم شدند (7=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">n</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">): گروه کنترل هیچ درمانی دریافت نکردند. گروه کنترل مثبت، </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">ATX</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> با دوز </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">&nbsp;mg/kg</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">20 دریافت کرد. گروه اتانول</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">،</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> اتانول 20% دریافت کرد. دو گروه تیمار </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">ATX</span></span></span><span style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> با دوزهای </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">&nbsp;mg/kg</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">20 و 10، که ابتدا اتانول 20% و سپس </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">ATX</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> دریافت کردند. تمام درمان‌ها به مدت 14 روز متوالی و به صورت خوراکی انجام شد. در این پژوهش، تست تشخیص شئ جدید (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">NORT</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">)، سطوح مالون‌دی‌آلدهید (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">MDA</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">) و دوپامین (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">DA</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">)، فعالیت کاتالاز (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">CAT</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">) و سوپراکسید دیسموتاز (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">SOD</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">) در هیپوکامپ موش‌ها <span style="background:yellow">ارزیابی شد</span>. مصرف اتانول باعث کاهش <span style="background:yellow">شاخص تمایز (شناسایی شی جدید) در </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:yellow"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">NORT</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">، فعالیت </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">CAT</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">SOD</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> و افزایش سطوح </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">DA</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">MDA</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> نسبت به گروه کنترل شد. درحالیکه، درمان </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">ATX</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> منجر به افزایش <span style="background:yellow">شاخص تمایز در </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:yellow"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">NORT</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">، فعالیت </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">CAT</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">SOD</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> و کاهش سطح </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">DA</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">MDA</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> در مقایسه با گروه اتانول شد. نتایج نشان داد که <span style="background:yellow">آستاگزانتین احتمالا بواسطه خواص آنتی‌اکسیدانی</span> می‌تواند نقایص شناختی و آسیب اکسیداتیو ناشی از اتانول را بهبود بخشد. بنابراین، آستاگزانتین می‌تواند به عنوان یک مکمل غذایی و دارویی بالقوه برای کاهش اختلالات ناشی از اتانول استفاده شود.</span></span></span></span></span></span></span></span> <span style="font-size:11pt"><span style="line-height:115%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Long-term ethanol consumption leads to the destruction of neurons in the central nervous system and cells in the hippocampus by causing oxidative stress. Astaxanthin (ATX) is a carotenoid that serves as an antioxidant and anti-inflammatory agent. This study sought to examine the impacts of astaxanthin on learning and memory impairments and oxidative damage within the hippocampus of mice induced by ethanol. 35 <span style="background:yellow">mice</span> were divided into five groups (n=7): the control group didn&#39;t receive any treatment. The positive control group received 20 mg/kg ATX. The ethanol group received 20% ethanol, and two ATX treatment groups received doses of 10 and 20 mg/kg, which first received 20% ethanol and then ATX. All treatments were done orally for 14 consecutive days. In this research, the novel object recognition test (NORT), malondialdehyde (MDA) and dopamine (DA) levels, and activities of catalase (CAT) and superoxide dismutase (SOD) were analyzed in the hippocampus of mice. Ethanol consumption decreased the <span style="background:yellow">discrimination index in NORT</span>, activities of CAT and SOD, and increased the levels of DA and MDA, compared to the control group. ATX treatment led to an increase in the discrimination index <span style="background:yellow">in NORT</span>, activities of CAT and SOD, and a decrease in the levels of DA and MDA, compared to the ethanol group. Results showed astaxanthin&#39;s antioxidant properties can improve ethanol-induced cognitive defects and oxidative damage. Therefore, astaxanthin can be used as a potential food and drug supplement to reduce ethanol-induced disorders.</span></span></span></span></span></span></span> آستاگزانتین, اتانول, هیپوکامپ, اختلال شناختی Astaxanthin, Ethanol, Hippocampus, Cognitive impairment 19 30 http://nbr.khu.ac.ir/browse.php?a_code=A-10-1591-1&slc_lang=fa&sid=1 Akbar Hajizadeh Moghaddam اکبر حاجی زاده مقدم a.hajizadeh@umz.ac.ir 100319475328460011025 100319475328460011025 Yes University of Mazandaran دانشگاه مازندران Farhad Samei فرهاد سامعی f.samei570@gmail.com 100319475328460011026 100319475328460011026 No University of Mazandaran دانشگاه مازندران Sedigheh Khanjani Jelodar صدیقه خانجانی جلودار s.khanjani66@yahoo.com 100319475328460011027 100319475328460011027 No University of Mazandaran دانشگاه مازندران Fatemeh Malekzadeh Estalkhi فاطمه ملک‌زاده اسطلخی fateme.malek94@gmail.com 100319475328460011028 100319475328460011028 No University of Mazandaran دانشگاه مازندران