یافته های نوین در علوم زیستی

Investigation of Phytochemical and Antioxidant Content of Arctium lappa Extract and its Antimicrobial Properties

نویسندگان

Parichehr Hanachi
Zahra Adibi
Zahra Gharari
Bahareh Attaran
Roshanak Zarrin Ghalami

Department of Biotechnology, Faculty of Biological Science, Alzahra University, Tehran, Iran.

10.22034/NBR.12.2.4
چکیده
Plant secondary metabolites like phenols and flavonoids neutralize free radicals and are linked to antioxidant and cytotoxic activities. Due to rising drug resistance in pathogens such as Salmonella paratyphi, Bacillus cereus, Escherichia coli, and Staphylococcus aureus, medicinal herbs are increasingly explored for antimicrobial properties. This study evaluated the total phenolic content (TPC), total flavonoid content (TFC), antioxidant capacity, and antibacterial effects of Arctium lappa. TPC, TFC, and antioxidant levels were measured using Folin-Ciocalteu, Aluminum chloride, FRAP, and DPPH assays, respectively. Antibacterial activities were assessed by microdilution broth and disc diffusion methods to determine MIC50 and MBC values. Results showed that A. lappa ethanolic extract, prepared with diluted water and the boiling method, had the highest flavonoid content (7.9 ± 0.40 mg/g DW). The leaves and flowers extract, using methanol and the boiling method, had the highest phenolic content (62.13 ± 0.73 mg/g DW) and antioxidant capacity. The ethanolic extract of A. lappa leaves also demonstrated the strongest antibacterial activity, with an MBC of 0.140 mg/mL and an 8 ± 0.4 mm inhibition zone against all tested bacteria. These findings suggest A. lappa bioactive compounds, particularly phenolics and flavonoids, offer promising potential for treating bacterial infections in pharmacognostic applications.

کلیدواژه‌ها

Antibacterial
antioxidant
Arctium lappa
Phenolic compounds
flavonoids
موضوعات

  1. 1.     Tanaka N, Kashiwada Y. Phytochemical studies on traditional herbal medicines based on the ethnopharmacological information obtained by field studies. J Nat Med. 2021;75(4):762-783. doi:10.1007/s11418-021-01545-7

    2.     Zarringhalami R, Hanachi P, Kaya E, AĞAN A, Agan K, Donmez M. Investigation of total phenolic content of Tilia dasystyla and Polygonatum orientale desf extracts and their cytotoxic effect on the osteogenic sarcoma (Saos-2) cancer cell line. International Journal of Cancer Management. 2020;13(2).

    3.     Zargaran A, Zarshenas MM, Karimi A, Yarmohammadi H. A review on the traditional Persian medicine in the management of diseases related to male infertility. Andrologia. 2016;48(8):850-9.

    4.     Azwanida N. A review on the extraction methods use in medicinal plants, principle, strength, and limitation. Med Aromat Plants. 2015;4(3):196.

    5.     Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S, Ju YH. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J Food Drug Anal. 2014;22(3):296-302.

    6.     Chemat F, Abert-Vian M, Fabiano-Tixier AS, Strube J, Uhlenbrock L. Green extraction of natural products. Origins, current status, and future challenges. Trends Analyt Chem. 2019;118:248-63.

    7.     Vinatoru M, Mason TJ, Calinescu I. Ultrasonically assisted extraction (UAE) and microwave assisted extraction (MAE) of functional compounds from plant materials. TrAC Trends Anal Chem. 2017;97:159-78.

    1. Dastmalchi K, Mozaffarian V, Naghizadeh F, Azizi M. A review on the genus Arctium (Asteraceae): A valuable resource for new bioactive compounds. Eur J Med Plants. 2016;12(1):1-12.
    2. Tzen JTC, Huang CC, Lin SM, Lee M. Properties and uses of Arctium lappa L. (greater burdock): A review. Nat Prod Commun. 2012;7(11):1515-20.
    3. Nascimento AP, Lima R, Almeida JF, Oliveira K, Almeida J, Ribeiro A, et al. Antioxidant activity and phytochemical composition of Arctium lappa L. and Arctium tomentosum Mill. Food Chem. 2018;265:162-8.
    4. Yosri N, Alsharif SM, Xiao J, Musharraf SG, Zhao C, Saeed A, Gao R, Said NS, Di Minno A, Daglia M, Guo Z. Arctium lappa (Burdock): Insights from ethnopharmacology potential, chemical constituents, clinical studies, pharmacological utility and nanomedicine. Biomedicine & Pharmacotherapy. 1 February 2023;158:114104..
    5. Hanachi, Parichehr & Zarrin Ghalami, Roshanak & Kaya, Ertugrul. (2020). Total Phenolic and Flavonoid Content and Antibacterial Properties of Polygonatum orientale Desf and Tilia dasystyla. Hormozgan Medical Journal. In Press. 10.5812/hmj.102953.
    6. 13.  Zarringhalami R, Hanachi P, Tamijani RR. Cytotoxic effect of tilia dasystyla and polygonatum orientale desf extracts on AGS and SKOV-3 cancer cell lines: effect of tilia dasystyla and polygonatum orientale desf on AGS and SKOV-3 cancer. Iranian Journal of Pharmaceutical Sciences. 2020;16(4):9-16.
    1. Eleftherios Halevas, Barbara Mavroidi, Maria Pelecanou, Antonios G. Hatzidimitriou,Structurally characterized zinc complexes of flavonoids chrysin and quercetin with antioxidant potential, Inorganica Chimica Acta, Volume 523, 2021, 120407,ISSN 0020-1693,
    2. Łukowski, Adrian & Jagiełło, Radosław & Robakowski, Piotr & Adamczyk, Dawid & Karolewski, Piotr. (2021). Adaptation of a simple method to determine the total terpenoid content in needles of coniferous trees. Plant Science. 314. 111090. 10.1016/j.plantsci.2021.111090.
    3. Hanachi P, Sadeghi Ali Abadi H, Ghorbani N, Zarringhalami R, Kiarostami K. Evaluation of antioxidant and anticancer effects of Lavandula angustifolia and Melissa officinalis on HeLa, OVCAR-3 and MCF-7 cancer cell lines. nbr. 2021; 8 (1) :20-30
    4. Al-marzook, F.A., & Omran, R. (2017). CYTOTOXIC ACTIVITY OF ALKALOID EXTRACTS OF DIFFERENT PLANTS AGAINST BREAST CANCER CELL LINE. Asian Journal of Pharmaceutical and Clinical Research, 10, 168-171.

    18.  Pirvu, L., I. Nicorescu, C. Hlevca, B. Albu and V. Nicorescu (2017). "Burdock (Arctium lappa) Leaf Extracts Increase the In Vitro Antimicrobial Efficacy of Common Antibiotics on Gram-positive and Gram-negative Bacteria." Open Chemistry 15(1): 92-102.

    19.  Pereira, J. V., D. C. Bergamo, J. O. Pereira, C. França Sde, R. C. Pietro and Y. T. Silva-Sousa (2005). "Antimicrobial activity of Arctium lappa constituents against microorganisms commonly found in endodontic infections." Braz Dent J 16(3): 192-196.

    20.    Guna G. Therapeutic value of Arctium lappa Linn.-a review. Asian J Pharmaceutical Clin Res. 2019;12:539.

    21.    Aroca S, Manzano A, Rivas E, et al. Bioactive compounds from Arctium lappa: An overview of their health benefits. Antioxidants. 2020;9(5):451.

    22.  Jahanban-Esfahlan A, et al. The impact of different extraction methods on the antioxidant and antibacterial activities of Arctium lappa leaves. Pharm Biol. 2018;56(1):1177-84.

    23.  Khosravi AR, et al. Antimicrobial activity of medicinal plants against multidrug-resistant bacteria. Pharmacogn Mag. 2016;12(46):282-6.

    24.  Petkova N, Hambarlyiska I, Tumbarski Y, Vrancheva R, Raeva M, Ivanov I. Phytochemical composition and antimicrobial properties of burdock (Arctium lappa L.) roots extracts. Biointerface Res. Appl. Chem. 2022;12:2826-42.

    25.  Lou, Z., Li, C., Kou, X., Yu, F., Wang, H., Smith, G. M., & Zhu, S. (2016). Antibacterial, Antibiofilm Effect of Burdock (Arctium lappa L.) Leaf Fraction and Its Efficiency in Meat Preservation. Journal of food protection, 79(8), 1404–1409.

    26.  Sultana S, et al. Phytochemical analysis and antimicrobial activity of ethanolic extract of Arctium lappa leaves against selected pathogens. BMC Complement Altern Med. 2018;18(1):39.

    27.  Mounnissami S, Ayyappan T, Karuppusamy S. A review on antimicrobial activity of herbal plants. World J Pharm Pharm Sci. 2016;5(6):1627-37.

    28.  Ghosh S, Ghosh A. Adverse effects of antibiotics and antibiotic resistance. Int J Res Pharm Sci. 2016;7(4):47-51.

    29.  Bouhaddou M, et al. Antimicrobial activity of selected medicinal plants against Staphylococcus aureus, Escherichia coli, and Salmonella spp. J Herbal Med. 2017;10:48-55.

    30.  Li Y, Wang Q, Wei HC, Liang YY, Niu FJ, Li KW, Zhou SJ, Zhou CZ. Fructus arctii: an overview on its traditional uses, pharmacology and phytochemistry. Journal of Pharmacy and Pharmacology. 2022 Mar 1;74(3):321-36.

  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 01 شهریور 1404

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