, B.bakhshandeh@ut.ac.ir
Abstract: (459 Views)
Stem cells have received serious attention from the medical industry based on their unique abilities and characteristics for precise and personalized medical approaches. To optimize and modify the behavioral characteristics of these cells for various clinical and research applications, it is usually necessary to create phenotypic changes resulting from permanent or transient genetic manipulations in these cells. Previously radiation and chemical reagents were used for random mutation induction in their genome but today, with the development of targeted modification tools like nucleases, it is possible to change only the desired gene and trait both inside the genome and episomally. Favorable gene transferring is accomplished with the help of different biological, chemical, or physical mediators and procedures. This literature review addressed the importance and necessity of stem cell manipulations, stem cell genetic modification procedures and advantages and disadvantages of each method, as well as available solutions to improve the efficiency and security of mentioned methods for clinical approaches.
Type of Study: Review |
Subject:
Biotechnology
Received: 2024/06/26 | Revised: 2025/05/21 | Accepted: 2025/05/21
Received: 2024/06/26 | Revised: 2025/05/21 | Accepted: 2025/05/21
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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