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Khodavandi P, Alizadeh F, Khodavandi A. The multidrug resistance and production of extended-spectrum beta-lactamases ctx-m, per and ver in Escherichia coli isolates derived from raw dairy samples. nbr 2020; 7 (1) :46-54
URL: http://nbr.khu.ac.ir/article-1-2931-en.html
The multidrug resistance and production of extended-spectrum beta-lactamases ctx-m, per and ver in Escherichia coli isolates derived from raw dairy samples
Pouria Khodavandi , Fahimeh Alizadeh , Alireza Khodavandi *
Department of Biology, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran , alireza_khodavandi@yahoo.com
Abstract:   (4392 Views)
The occurrence of extended-spectrum beta-lactamases-producing bacteria is an important public health issue. The aim of this study was to investigate phenotypic and genotypic characteristics regarding the presence of extended spectrum β-lactamase ctx-m, per and ver producing Escherichia coli isolated from raw dairy samples. For this purpose, E. coli were isolated from 247 raw dairy samples (milk and cheese) in Yasooj in 2015-2017, and the isolates were screened for antibiotic resistance, extended spectrum β-lactamase and the presence of ctx-m, per and ver. In total, 200 isolates were selected. The highest frequency of resistance in isolates was against tetracycline (96.5%) and ampicillin (95.5%) antibiotics and the lowest against imipenem (12.5%), In addition, multidrug resistance against four or more antibiotics was observed in some isolates. Extended spectrum β-lactamase resistance was detected in 86 isolates (43%) and ctx-m, per and ver genes were detected in 82, 0 and 7 E. coli isolates, respectively. These findings demonstrated that raw dairy products may be reservoirs for the dissemination of β-lactam antibiotics and that resistance genes could be transmitted to humans through the food chain.

 
Keywords: antibiotic, bacteria, gene, raw milk, unprocessed cheese
Full-Text [PDF 871 kb]   (1378 Downloads)    
Type of Study: Original Article | Subject: Microbiology
Received: 2017/08/12 | Revised: 2021/06/1 | Accepted: 2019/11/4 | Published: 2020/03/31 | ePublished: 2020/03/31
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